Mouse Embryology Protocols

Protocols and information related to mouse embryology.

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Mouse Embryology Protocols Protocol Links Sort by: Hits | Alphabetical

Blastocyst Embryo Transfer into Pseudopregnant Mouse Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp6.html

Protocol for blastocyst embryo transfer into pseudopregnant recipient female mouse. - [Read Blastocyst Embryo Transfer into Pseudopregnant Mouse Protocol]

Breeding Mice Protocol - http://members.cox.net/microinjectionworkshop/technical/breeding.html

Information and protocol for breeding mice. - [Read Breeding Mice Protocol]

Collecting Blastocysts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4360

Protocol describes a method for collecting blastocysts from pregnant female mice at 3.5 to 4.5 days post coitum (dpc). The blastocysts can then be injected with embryonic stem cells to make chimeras. - [Read Collecting Blastocysts Protocol]

Collecting Zygotes and Removing Cumulus Cells With Hyaluronidase Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4358

Protocol describes a method for collecting zygotes from pregnant female mice. The zygotes can then be used for microinjection. - [Read Collecting Zygotes and Removing Cumulus Cells With Hyaluronidase Protocol]

Detection of Estrus in Mice - http://members.cox.net/microinjectionworkshop/technical/estrus.html

Information on the detection of estrus in mice. - [Read Detection of Estrus in Mice]

Disaggregating Cleavage-Stage Embryos and the Inner Cell Mass of Blastocysts into Individual Cells - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4427

Protocol describes a method for disaggregating embryos and the ICM of blastocysts into individual cells. - [Read Disaggregating Cleavage-Stage Embryos and the Inner Cell Mass of Blastocysts into Individual Cells]

Fixation of Mouse Embryos and Tissues Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4702

Protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution. - [Read Fixation of Mouse Embryos and Tissues Protocol]

Isolating Mice Extraembryonic Membranes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4367

Isolation of extraembryonic tissues allows one to study normal mouse development as well as the molecular basis of defects which cause fetal death. This protocol describes a method for isolating extraembryonic membranes from pregnant mice. - [Read Isolating Mice Extraembryonic Membranes Protocol]

Isolation of Mouse Embryos Video Clip - http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Mouse/mouse_dissection.html

Isolation of mouse embryos video clip. - [Read Isolation of Mouse Embryos Video Clip]

Making Holding Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4394

Protocol describes a method for making holding pipettes, to be used for microinjection. - [Read Making Holding Pipettes Protocol]

Making Injection Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4395

Protocol describes the determination of useful settings with the Sutter Puller P-97 to make injection pipettes for microinjection. - [Read Making Injection Pipettes Protocol]

Making Pipettes From Hard Glass Capillaries Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4354

Protocol describes a method for making pipettes from hard glass capillaries. - [Read Making Pipettes From Hard Glass Capillaries Protocol]

Mouse Embryo Transfer into Pseudopregnant Recipient Protocol - http://members.cox.net/microinjectionworkshop/technical/tnp2.html

1-cell embryo transfer into pseudopregnant recipient female mouse. - [Read Mouse Embryo Transfer into Pseudopregnant Recipient Protocol]

Planning an Effective Mouse Colony - http://members.cox.net/microinjectionworkshop/technical/tgcolny1.html

Considerations in planning an effective mouse colony for transgenic production. Includes: Mouse strain; Colony size; Efficiency of superovulation; Parental Suitability; Pseudo-pregnancy. - [Read Planning an Effective Mouse Colony]

Preparing Siliconized Pipettes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4355

Protocol describes a method for preparing siliconized pipettes. Such pipettes minimize the loss of embryos or embryonic tissues during transfer. - [Read Preparing Siliconized Pipettes Protocol]

Sectioning Mouse Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4703

Protocol provides methods and tips for sectioning mouse embryos and transferring the sections to a microscope slide. - [Read Sectioning Mouse Embryos Protocol]

Transfer of Injected Blastocysts to Pseudo Pregnant Mice Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1118

Blastocyst transfer is usually performed 24 hours after aggregation, when the morulae have become expanded blastocysts, and on the same day as injection. A little time is given between injection and transfer to allow the blastocysts to re-expand. Includes: The Mouse Recipient; The Transfer. - [Read Transfer of Injected Blastocysts to Pseudo Pregnant Mice Protocol]

Whole Mount In Situ Hybridization of Mouse Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/in_situ.html

Protocol for whole mount in situ hybridization of mouse. Incluses: Preparation of the probe; General Comments; Preparation of embryos; In Situ hybridization. - [Read Whole Mount In Situ Hybridization of Mouse Protocol]