Assembling Chimeras Mouse Protocols

Protocols and information related to assembling mouse chimeras.

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Assembling Aggregates between Diploid and Tetraploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4425

Protocol describes a method for producing diploid embryo-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for diploid embryo-diploid embryo aggregation. The resulting chimeras are useful for phenotypic analysis when an induced mutation has an extraembryonic phenotype. - [Read Assembling Aggregates between Diploid and Tetraploid Embryos Protocol]

Assembling Aggregates between Diploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4423

Protocol describes a method for assembling aggregates between diploid embryos. If embryos from a heterozygous mutant intercross are aggregated with wild-type embryos, the resulting chimeras can be used for analyzing mutant phenotypes. - [Read Assembling Aggregates between Diploid Embryos Protocol]

Assembling Aggregates between Embryonic Stem (ES) Cells and Diploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4424

Protocol describes a method for assembling aggregates between ES cells and diploid embryos. The resulting chimeras are useful for separating certain extraembryonic phenotypes from phenotypes in the embryo proper, since the diploid embryo contributes to all parts of the conceptus, but the ES cell component does not contribute to the trophoblast or yolk sac endoderm. - [Read Assembling Aggregates between Embryonic Stem (ES) Cells and Diploid Embryos Protocol]

Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4426

Protocol describes a method for producing ES cell-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for ES cell-diploid embryo aggregation in which diploid embryos are replaced with tetraploid embryos. The resulting chimeras can be used to analyze the embryonic versus extraembryonic phenotype of a mutation. - [Read Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos Protocol]

Preparing the Aggregation Plate Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4420

Protocol describes the preparation of aggregation plates, which will then be used for the production of aggregation chimeras. - [Read Preparing the Aggregation Plate Protocol]

Production of Tetraploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4422

Protocol describes the production of tetraploid embryos by electrofusion. - [Read Production of Tetraploid Embryos Protocol]

Removal of Zona Pellucida Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4421

Protocol describes a simple method for removal of the zona pellucida of preimplantation mouse embryos using an acidified Tyrode’s solution. - [Read Removal of Zona Pellucida Protocol]