Mouse Protocols

Protocols and information related to mice. Including: Mouse Surgical Procedures; Mouse Reproductive System Surgery; Mouse Organ Removal Surgery; Assembling Chimeras; Mouse Embryology; Mouse Genetics; Mouse Cell Culture.

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Mouse Protocols Categories

Assembling Chimeras Mouse Protocols (7)

Mouse Cell Culture Protocols (11)

Mouse Embryology Protocols (18)

Mouse Genetics Protocols (8)

Mouse Organ Removal Surgery Protocols (3)

Mouse Reproductive System Surgery Protocols (9)

Mouse Surgical Procedures Protocols (5)

Mouse Protocols Protocol Links Sort by: Hits | Alphabetical

BDA Mouse Lineage Tracing on Sections Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/Pdfs/BDA%20staining%20on%20section.pdf

Protocol describes the strongest way for lineage tracing on sections by using biotin dextran amine. - [Read BDA Mouse Lineage Tracing on Sections Protocol]

Calcium Dependent by Pentraxins to Ligands, and Effect of C-reactive Protein on Leptin Action - http://www.natureprotocols.com/2007/01/24/calcium_dependent_by_pentraxin.php

Protocol for calcium dependent by pentraxins to ligands immobilized on agarose, and effect of C-reactive protein on leptin action in mice in vivo. Includes: Testing for binding of CRP to immobilized leptin; Testing for binding of radiolabelled leptin by immobilized CRP, SAP and leptin receptor; Testing for effect of human CRP on human leptin action in mice. - [Read Calcium Dependent by Pentraxins to Ligands, and Effect of C-reactive Protein on Leptin Action]

Collection of Mouse Peritoneal Cells Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/mousepathology/CollectPerC.html

Protocol for the collection of mouse peritoneal cells. - [Read Collection of Mouse Peritoneal Cells Protocol]

Dissociation of Mouse Spleen and Hemopoietic Tissue Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/mousepathology/DissociationSpleen.html

Protocol for the dissociation of mouse spleen and hemopoietic tissue. - [Read Dissociation of Mouse Spleen and Hemopoietic Tissue Protocol]

Effects of Cyclopamine on Mouse Vibrissa Follicle Explants - http://www.swarthmore.edu/NatSci/sgilber1/DB_lab/Mouse/Vibrissa02.html

Protocol to test the effects of cyclopamine on mouse vibrissa follicle explants. - [Read Effects of Cyclopamine on Mouse Vibrissa Follicle Explants]

Extraction of the SecinH3 from Mouse Liver Protocol - http://www.natureprotocols.com/2006/12/15/extraction_of_the_secinh3_from.php

Mice fed with the cytohesin inhibitor SecinH3 for two days develop hepatic insulin resistance that can be identified by reduced liver glycogen levels, increased serum insulin and ketone body levels and decreased serum non-esterified fatty acid. To confirm the presence and identity of SecinH3 in mouse liver, we extracted the compound from liver homogenates with chloroform and identified it by LC/MS. - [Read Extraction of the SecinH3 from Mouse Liver Protocol]

Immunohistology of Mouse Spleens Protocol - http://www.natureprotocols.com/2007/01/22/immunohistology_of_mouse_splee.php

Protocol for the immunohistology of mouse spleens. - [Read Immunohistology of Mouse Spleens Protocol]

Isolation of Mouse Intrahepatic Lymphocytes Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E66370706B026EE3B53EB4B32AD4A09&objectid=6674D486BDDA02EC25183A2F0D9BED25

Provides two protocols that may be used to isolate IHL. One can be used to isolate IHL from multiple livers in parallel, whereas the more elaborate alternate protocol yields more cells per liver but is more appropriately used to recover the IHL from a single liver. - [Read Isolation of Mouse Intrahepatic Lymphocytes Protocol]

Isolation of Murine Macrophages Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6632549C959D0FFF7FD119F10D62D9&objectid=667572BADF718B4599BE6AE4ADD48CB3

Protocol that investigates macrophage activation using a more homogeneous population of cells, macrophages derived from immature progenitor cells in bone marrow can be studied. A second protocol describes the isolation of bone marrow-derived progenitor cells and propagation of these immature macrophages by CSFs or IL-3. - [Read Isolation of Murine Macrophages Protocol]

Natural Pathogens of Laboratory Mice and Rats - http://members.cox.net/microinjectionworkshop/technical/pathogen.html

For all the transgenic or other mouse researchers out there who have had to explain why colony infections are not just bad for the welfare of the animals concerned, but may invalidate the ongoing studies as well. - [Read Natural Pathogens of Laboratory Mice and Rats]