Microarray Analysis Protocols

Protocols and information related to microarray analysis.

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Anatomy of a Comparative Gene Expression Study - http://www.cs.wustl.edu/~jbuhler//research/array/

Information on anatomy of a comparative gene expression study. Includes: Choosing cell populations; mRNA Extraction and Reverse Transcription; Fluorescent labeling of cDNA's; Hybridization of DNA microarray; Scanning the hybridized array; Interpreting the scanned image. - [Read Anatomy of a Comparative Gene Expression Study]

aRNA Labeling and Hybridization Protocol - http://www.stanford.edu/group/sjeffreylab/PDF%20Files/aRNA_labeling.pdf

Protocol for aRNA labeling and hybridization. - [Read aRNA Labeling and Hybridization Protocol]

Genome-Wide Analysis Protocol - http://www.natureprotocols.com/2006/06/29/genomewide_analysis_of_data_ge.php

Genome-wide analysis of data generated on the Affymetrix 10K Xba 142 arrays for identification of regions with high probability to contain genes responsible for Micronodular (non-pigmented) Adrenocortical Hyperplasia. - [Read Genome-Wide Analysis Protocol]

Isolation of Total RNA From E. Coli for Microarrays Protocol - http://bugarrays.stanford.edu/protocols/rna/Total_RNA_from_Ecoli.pdf

Protocol for isolation of total RNA from E. coli for microarrays. Includes: Harvesting total RNA; Dnase I treatment of RNA samples. - [Read Isolation of Total RNA From E. Coli for Microarrays Protocol]

Microarray Analysis of Highly Purified Human Decidual NK Cells Protocol - http://www.natureprotocols.com/2006/08/07/microarray_analysis_of_highly.php

Description of gene array analysis and normalization - [Read Microarray Analysis of Highly Purified Human Decidual NK Cells Protocol]

Microarray Protocols - http://www.erasmusmc.nl/interne_oncologie/res/endo/Protocol%20for%20RNA%20amplification%2C%20labelling%20and%20hybridization%20to%20%20microarray.pdf

Protocols for microarrays. Includes: RNA amplification by in vitro transcription; cDNA labeling; Hybridization of cDNA microarrays. - [Read Microarray Protocols]

Preparation of B-Lymphocyte RNA for Microarray Analysis Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000009.pdf?pid=PP00000009

Protocol permits the isolation of at least 5 µg of total RNA from a sample of purified mouse splenic B lymphocytes Isolation of Resting B Lymphocytes from Sixteen Mouse Spleens. - [Read Preparation of B-Lymphocyte RNA for Microarray Analysis Protocol]

Preparation of Myocyte RNA for Microarray Analysis Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000133.pdf?pid=PP00000133

Protocol permits the isolation of at least 3 µg of total RNA from approximately 150,000 cultured cardiac myocytes from adult mice. Includes: Treatment of Samples and Termination of Reactions; Isolation of RNA; Use of Environmental Chamber. - [Read Preparation of Myocyte RNA for Microarray Analysis Protocol]