zygotes Protocols

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Collecting Zygotes and Removing Cumulus Cells With Hyaluronidase Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4358

Category: Mouse Protocols: Mouse Embryology Protocols

Protocol describes a method for collecting zygotes from pregnant female mice. The zygotes can then be used for microinjection. - [Read Collecting Zygotes and Removing Cumulus Cells With Hyaluronidase Protocol]

Making a Tetrad Dissection Needle Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4182

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Microneedles attached to micromanipulators are used in the dissection of tetrads, isolation of zygotes from populations of mating haploid cells, and manipulation of individual cells. - [Read Making a Tetrad Dissection Needle Protocol]

Microinjection of Mouse Zygotes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4397

Category: Microinjection Protocols: Microinjection of DNA Protocols

This protocol describes a method for microinjection of mouse zygotes to produce transgenic mice. - [Read Microinjection of Mouse Zygotes Protocol]

Microinjection Setup Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4396

Category: Microinjection Protocols: Microinjection Setup Protocols

This protocol describes the microinjection setup for injecting mouse zygotes to produce transgenic mice. - [Read Microinjection Setup Protocol]

Picking Zygotes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4183

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Zygotes can be identified by their unique morphology. They can be easily separated away from nonmated cells using a micromanipulator. This method provides an alternative to the selection of diploid cells on a medium that prevents the growth of haploid parent cells. - [Read Picking Zygotes Protocol]