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Deglycosylation of Glycoproteins Using Endoglycosidases - http://www.glycotech.com/protocols/Proto3.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Deglycosylation steps are different with every glycoprotein and must be determined empirically (Methods in Enzymology, 1994, 230: 44-57). A typical test reaction of human transferrin is described. T.H. Plummer, Jr. and A.L. Tarentino, Department of Biochemistry, New York State Department of Health, Wadsworth Center for Laboratories and Research, Albany, New York - [Read Deglycosylation of Glycoproteins Using Endoglycosidases]

Drosophila Larval Chemotaxis Assays - http://vosshall.rockefeller.edu/protocols/LarvalChemotaxisAssay.pdf

Category: Immunology: Chemotaxis

Drosophila Larval Chemotaxis Assays. Larva harvesting. Olfactory tests on control larvae. Measuring response. Leslie Vosshall Laboratory, Rockefeller University, New York. - [Read Drosophila Larval Chemotaxis Assays]

High Efficiency FCC Preparation and Tx Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F4.html

Category: Competent Cells Protocols

This is an easy and straightforward protocol that gives efficiencies of 106 to 107 cfu/mg of plasmid DNA. A growth curve is required for each strain that is prepared.
- [Read High Efficiency FCC Preparation and Tx Protocol]