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Yeast RNA Isolation: Large-Scale Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4154

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol describes large-scale yeast RNA isolation. It is designed to yield more than 10 mg of total nucleic acid and 300-400 µg of poly(A)-selected mRNA from each 500-ml culture. - [Read Yeast RNA Isolation: Large-Scale Protocol]

Yeast RNA Isolation: Small-Scale Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4155

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol describes rapid, small-scale yeast RNA isolation. It is based on the work of Schmitt et al. (1990). Note that all containers should be washed in Rnase Away (Invitrogen) or dry baked for 24 hours at 160°C. - [Read Yeast RNA Isolation: Small-Scale Protocol]

Yeast Transformation (for LexA Library Screen) Protocol - http://www.natureprotocols.com/2006/12/15/yeast_transformation_for_lexa.php

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for Yeast transformation (for LexA Library Screen). - [Read Yeast Transformation (for LexA Library Screen) Protocol]

Yeast Transformation (high efficiency) Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/dmso_yeast_transform.html

Category: Yeast Protocols: Yeast Transformation Protocols: High Efficiency Yeast Transformation Protocols

Protocol for high efficiency Yeast transformation. - [Read Yeast Transformation (high efficiency) Protocol]

Yeast Transformation Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/yeast-trafo.html

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for Yeast transformation. - [Read Yeast Transformation Protocol]

Yeast Two Hybrid Assay: A Fishing Tale - http://www.bioteach.ubc.ca/Journal/V01I01/8188yeast.pdf

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Theory, Galactose metabolism, The Pros and Cons of the Two hybrid Assay. Troubleshooting and Tips for Hybrid assay. Sobhanifar BioTeach Journal 2003. - [Read Yeast Two Hybrid Assay: A Fishing Tale]

Yeast Two-Hybrid Screen with Library and Bait - http://faculty.virginia.edu/mirmira/resources_files/Protocols/YEAST%20TWO.htm

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

yeast transformed with bait vector. yeast culture. selection plates. Mirmira, Univ. Virginia. - [Read Yeast Two-Hybrid Screen with Library and Bait]

Yeast Two-hybrid Systems Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3889

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

Protocol for Yeast two-hybrid systems. - [Read Yeast Two-hybrid Systems Protocol]

Yeast Two-Hybrid: State of the Art - http://www.biologicalprocedures.com/bpo/arts/1/16/m16.htm

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Yeast Two-Hybrid: State of the Art. Paper on Protein-protein interactions and the two-hybrid protocol. Wim Van Criekinge et al., BPO. - [Read Yeast Two-Hybrid: State of the Art]

Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4166

Category: Yeast Protocols: Yeast Staining Protocols

Various vital stains are available for yeast. This protocol describes the staining of chitin and bud. - [Read Yeast Vital Stains: Calcofluor Staining of Chitin and Bud Scars Protocol]

Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4163

Category: Yeast Protocols: Yeast Staining Protocols

Protocol describes the staining of nuclear and mitochondrial DNA with DAPI (4',6-diamidino-2-phenylindole). - [Read Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA Protocol]

Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4164

Category: Cell Organelle Protocols: Mitochondria Protocols

Protocol describes the visualization of mitochondria with DiOC6 or DiIC5(3). - [Read Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5 Protocol]

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Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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