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Yeast Chromatin Immunoprecipitation (ChIP) - http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/hahnlab_ChIP_method.html

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Chromatin IP Method, ChIP buffers, Notes on ChIP Method, Quantative PCR using 32P dCTP. Hahn Lab. - [Read Yeast Chromatin Immunoprecipitation (ChIP)]

Yeast Chromatin Immunoprecipitation (ChIP) Assay Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4642

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Immunoprecipitation Protocols

Protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of this assay. - [Read Yeast Chromatin Immunoprecipitation (ChIP) Assay Protocol]

Yeast Chromosomal DNA Prep Protocol - http://www.fhcrc.org/science/labs/breeden/Methods/chromosomal-DNA.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast chromosomal DNA prep. - [Read Yeast Chromosomal DNA Prep Protocol]

Yeast Colony PCR - http://www.fhcrc.org/science/labs/breeden/Methods/colonyPCR.html

Category: PCR Protocols: Colony PCR

Yeast colony PCR (analytic). Breeden Lab Fred Hutchinson Cancer Research Center - [Read Yeast Colony PCR]

Yeast Colony PCR - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4170

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

This method for yeast colony PCR is used to test for the presence of target sequences in cells. - [Read Yeast Colony PCR]

Yeast Colony PCR Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/ypcr.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for Yeast colony PCR. Includes: Quick SDS extraction protocol. - [Read Yeast Colony PCR Protocol]

Yeast Colony PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4170

Category: PCR Protocols: Colony PCR

Protocol for yeast colony PCR. This method for yeast colony PCR is used to test for the presence of target sequences in cells. - [Read Yeast Colony PCR Protocol]

Yeast DNA Isolation Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/yacs.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Yeast DNA Isolation Protocol for FISH. Sanger Institute. - [Read Yeast DNA Isolation Protocol for FISH]

Yeast DNA Isolation: Midiprep Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4147

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast DNA isolation. Protocol describes the preparation of DNA from a 40-ml liquid culture. - [Read Yeast DNA Isolation: Midiprep Protocol]

Yeast DNA Isolation: Miniprep Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4148

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the preparation of DNA from a 5-ml liquid culture of yeast. - [Read Yeast DNA Isolation: Miniprep Protocol]

Yeast Genomic DNA Isolation Procedure - http://www.upstate.edu/biochem/amberg/protocols/yeast_genomic_DNA.html

Category: DNA Protocols: DNA Extraction Protocols

Yeast Genomic DNA Isolation David Amberg, Upstate Medical University. - [Read Yeast Genomic DNA Isolation Procedure]

Yeast Growth Rate Cytotoxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/33.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

The proliferation rate of the yeast, Saccharomyces cerevisiae, may be regarded as an overall indicator of the physiological status of the cell. Therefore, the effect of various toxic substances on different cell functions will be reflected by changes in the rate of proliferation. It is possible to determine the toxicity of a test substance simply by measuring cell density. - [Read Yeast Growth Rate Cytotoxicity Test]

Yeast IF without Dehydration - http://genomics.princeton.edu/botstein/protocols/non_MeOH_IF.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast IF without Dehydration. Botstein Lab. Protocol is a modified version of the protocol from Mark Rose in the CSH Yeast Genetics Course Manual. - [Read Yeast IF without Dehydration]

Yeast Immunofluorescence protocol - http://clone.concordia.ca/yeast2/protocols/Immunofluorescence_protocol.pdf

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast Immunofluorescence protocol, includes recipes for Immunofluorescence Buffers. Concordia. - [Read Yeast Immunofluorescence protocol]

Yeast Immunofluorescence Protocol - http://www.biochem.emory.edu/labs/acorbe2/immuno.html

Category: Yeast Protocols: Yeast Staining Protocols

Protocol for Yeast immunofluorescence. - [Read Yeast Immunofluorescence Protocol]

Yeast Immunofluorescence with methanol - http://www.upstate.edu/biochem/amberg/protocols/MeOH_IF.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast IF with Methanol/Acetone Dehydration. Amberg Lab, Upstate Medical University. - [Read Yeast Immunofluorescence with methanol]

Yeast Immunofluoresence Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4167

Category: Yeast Protocols: Yeast Staining Protocols

This protocol describes a method based on that of Pringle for immunofluorescent staining of yeast, as would be done in gene replacement experiments. - [Read Yeast Immunofluoresence Protocol]

Yeast Media Protocols - http://genomics.princeton.edu/botstein/protocols.shtml

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Protocols for yeast media including: YNB1 1.1x agar, YNB2 1.1x liquid, YNB3 agar, YNB4 liquid, YNB5 Agarose, YP1 1.1x agar pH6, YP2 1.1x agar, YP3 1.1x liquid pH6, YP4 1.1x liquid, YPD1 agar pH6, YPD2 agar, YPD3 liquid pH6, YPD4 liquid. - [Read Yeast Media Protocols]

Yeast Media, Solutions and Stocks Protocols - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast11.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast Media, Solutions and Stocks Protocols. Includes: Yeast Media; AHC Medium; AHC plates; SD Medium; SD plates; Sorbitol-Agar Medium; Sorbitol-Agar Medium Top Agar; Sporulation Medium Plates; YPD Medium; YPD plates; Synthetic Media Supplements. - [Read Yeast Media, Solutions and Stocks Protocols]

Yeast Miniprep (Plasmid Rescue) Protocol - http://www.biochem.emory.edu/labs/acorbe2/yeastrescue.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for Yeast miniprep (plasmid rescue). - [Read Yeast Miniprep (Plasmid Rescue) Protocol]

Yeast Nuclear Extract (Small Scale) - http://www.fhcrc.org/science/labs/hahn/methods/biochem_meth/polii_nuc_ext.html

Category: Protein Protocols: Nuclear Extraction Protocols

Nuclear Extract preparation protocols, Buffers and solutions for nuclear extracts. Hahn Lab - [Read Yeast Nuclear Extract (Small Scale)]

Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Category: Yeast Protocols

Plasma membranes are isolated from the yeast Saccharomyces cerevisiae. The cell wall is initially digested by helicase, followed by hypoosmotic lysis and homogenization. Membranes are prepared by subsequent differential centrifugation. The activity of the H+-ATPase is then determined by measuring the amount of inorganic phosphate released from ATP. - [Read Yeast Plasma Membrane H+ -ATPASE Toxcity Test Protocol]

Yeast Plasma Membrane H+ -ATPASE Toxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

Accumulation of lipophilic substances, many of which may be environmental chemicals, affects the membrane lipid order and consequently affects the functions of these proteins. Since, the function of important cellular proteins, such as the H+-ATPase strongly depends upon the integrity of the lipid bilayer, the activity of the H+-ATPase may be used as a sensitive indicator of the effect that a chemical may have on the viability of the cell. - [Read Yeast Plasma Membrane H+ -ATPASE Toxicity Test]

Yeast Plates Protocols - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_plates.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast plates protocols. Includes: Glucose Complete Plates (1 liter); YPD Plates (1 liter); 5-FOA plates (0.5 liter). - [Read Yeast Plates Protocols]

Yeast Protein Extracts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4152

Category: Yeast Protocols: Yeast Protein Protocols

Protocol is a simple, reliable method for the preparation of yeast protein extracts for analysis by polyacrylamide gel electrophoresis (PAGE) and Western blotting. - [Read Yeast Protein Extracts Protocol]

Articles (1-5 of 5)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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