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Sizing of YAC Clones Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast8.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for sizing of YAC clones. To determine the size of a yeast artificial chromosome within the background of the normal yeast chromosomal complement. - [Read Sizing of YAC Clones Protocol]

Small-scale Preparations of Yeast DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3299

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

This method reproducibly yields several micrograms of yeast DNA that can be efficiently cleaved by restriction enzymes and used as a template in PCR. - [Read Small-scale Preparations of Yeast DNA Protocol]

Solutions for Hartwell's Complete (HC) Media - http://www.fhcrc.org/science/labs/gottschling/yeast/hc.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Solutions for hartwell's complete (HC) media. Includes: 10X HC (7 Dropout Amino Acid Liquid); 10X YNB; Amino Acid Solutions. - [Read Solutions for Hartwell's Complete (HC) Media]

Sporulation and Dissection of Yeast a/alpha Diploids Protocol - http://www.fhcrc.org/science/labs/hahn/methods/genetic_meth/yeast_sporulation.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocol for sporulation and dissection of yeast a/alpha diploids. - [Read Sporulation and Dissection of Yeast a/alpha Diploids Protocol]

Sporulation Protocols - http://www.fhcrc.org/science/labs/gottschling/yeast/spo.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Protocols for sporulation. Includes yeast vivisection. - [Read Sporulation Protocols]

Staining Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4520

Category: Yeast Protocols: Yeast Staining Protocols

Protocol for staining Yeast. - [Read Staining Yeast Protocol]

Streaking Yeast Stocks Protocols - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast1.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Some yeast strains are unstable (e. g., small YAC-bearing strains) and need to be repurified by streaking on an agar plate and then verifying the genetic content of the isolated colony before proceeding. In cases where the strain is unstable, plan to streak the cells onto the selective medium to retain the desired stock, (however, most strains can be streaked onto the complete medium, YPD). - [Read Streaking Yeast Stocks Protocols]

Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast9.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for the subcloning of Yeast artificial chromosome into phage lambda. To subclone the large insert fragment of human DNA contained within a YAC into a bacteriophage lambda vector. The subclones are 15 to 23 kb in size, and can be used to identify new polymorphic markers from a known region of the genome, to map a specific locus, and/or to screen other libraries. - [Read Subcloning of Yeast Artificial Chromosomes into Phage Lambda Protocol]

Tandem Affinity Purification (TAP) Protocol - http://depts.washington.edu/~yeastrc/ms_tap1.htm

Category: Mass Spectrometry Protocols

Tandem Affinity Purification (TAP) Protocol. Modified from Rigaut et al. 1999. Univ.Washington Yeast Resource Center. - [Read Tandem Affinity Purification (TAP) Protocol]

TAP Fusion Protein Purification from Yeast Protocol - http://www.med.unc.edu/~hdohlman/TAP.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Immunoprecipitation Protocols

Protocol for TAP fusion protein purification from Yeast. - [Read TAP Fusion Protein Purification from Yeast Protocol]

Tetrad Dissection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4181

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

This protocol describes dissection of yeast tetrads. Tetrad analysis is useful for linkage studies and for constructing strains for genetic and biochemical experiments. - [Read Tetrad Dissection Protocol]

TSY (Trypticase Soy Yeast) Agar Protocol - http://www.thelabrat.com/protocols/TSYagar.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Agar Cell Culture Media Protocols

Protocol for TSY (Trypticase Soy Yeast) agar. - [Read TSY (Trypticase Soy Yeast) Agar Protocol]

TSY (Trypticase Soy Yeast) Broth Protocol - http://www.thelabrat.com/protocols/TSYbroth.shtml

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Broth Culture Media Protocols

Protocol for TSY (Trypticase Soy Yeast). - [Read TSY (Trypticase Soy Yeast) Broth Protocol]

Two Hybrid System Transformation Protocol - http://www.umanitoba.ca/faculties/medicine/biochem/gietz/2HS.html

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

High Efficiency Transformation of a yeast strain requiring maintenance of a plasmid. - [Read Two Hybrid System Transformation Protocol]

Two-hybrid Systems Stage 1: Characterization of a Bait-LexA Fusion Protein Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3895

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

Protocol describes how to generate a plasmid construct (pBAIT) that expresses a target protein fused to the bacterial LexA protein. PBAIT is cotransformed into yeast with a lexAop-lacZ reporter plasmid carrying the bacterial lacZ gene under the control of the lexA operator. The recipient yeast strain contains a chromosomally integrated leu2 reporter gene, also under the control of the lexA operator. - [Read Two-hybrid Systems Stage 1: Characterization of a Bait-LexA Fusion Protein Protocol]

Two-hybrid Systems Stage 2: Selecting an Interactor Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3888

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

In this stage of the protocol, a mammalian cDNA library constructed in a plasmid such as pJG4-5 is transformed into yeast strains containing pBAIT and the lexAop-lacZ reporter plasmid. PJG4-5 expresses the cloned cDNAs from a cassette containing a transcriptional activation domain and other moieties under the control of the yeast GAL1 promoter. - [Read Two-hybrid Systems Stage 2: Selecting an Interactor Protocol]

Ubiquitin Assay Protocol for Yeast - http://tanseylab.cshl.edu/pdf/His_Ub_Yeast.pdf

Category: Protein Protocols: Protein Ubiquitination Protocols

Ubiquitin Assay Protocol for Yeast. Tansey Lab. - [Read Ubiquitin Assay Protocol for Yeast]

Vectorette PCR of Yeast DNA Protocol - http://genomics.princeton.edu/botstein/protocols/vectorette.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for vectorette PCR of Yeast DNA. - [Read Vectorette PCR of Yeast DNA Protocol]

Working with Yeast Artificial Chromosomes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3297

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol outlines a procedure to validate and store yeast strains carrying YACs. - [Read Working with Yeast Artificial Chromosomes Protocol]

YAC Transfer by KAR1Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/YEAST/YACTransfer.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for YAC transfer by KAR1. Includes: YAC transfer by Kar1 mating; Transfer YAC from donor cells into YPH 925; Transfer YAC from YPH925 into recipient cells. - [Read YAC Transfer by KAR1Protocol]

YC Dropout Plate Supplementation Table - http://www.fhcrc.org/science/labs/gottschling/yeast/ycsup.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

YC Dropout Plate Supplementation Table. - [Read YC Dropout Plate Supplementation Table]

Yeast and Bacterial Colony PCR Protocol. - http://www.upstate.edu/biochem/amberg/protocols/colony_pcr.html

Category: PCR Protocols: Colony PCR

Yeast and Bacterial Colony PCR Protocol. David Amberg SUNY Upstate Medical University - [Read Yeast and Bacterial Colony PCR Protocol.]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Cell Biology and Cell Culture: Cell Cycle Protocols

Protocol for yeast cell cycle by flow cytometry. Includes procedure for dyes: Propidium Iodide and Sytox Green. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Cycle Analysis Protocols

Protocol for yeast cell cycle by flow cytometry. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Yeast Cell Cycle by Flow Cytometry Protocol - http://pingu.salk.edu/flow/protocols/ycc.html

Category: Yeast Protocols

Protocol for Yeast cell cycle by flow cytometry. - [Read Yeast Cell Cycle by Flow Cytometry Protocol]

Articles (1-5 of 5)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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