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Plasmid Transformation of Yeast Colonies Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4146

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for plasmid transformation of Yeast colonies. Protocol can be used if high efficiency is not needed. - [Read Plasmid Transformation of Yeast Colonies Protocol]

Plate Assay for Carboxypeptidase Y in Yeast Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4161

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for plate assay for carboxypeptidase Y in Yeast cells. - [Read Plate Assay for Carboxypeptidase Y in Yeast Cells Protocol]

Preparation of cell lysates from yeast by enzymatic digestion of the cell wall - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_yeast_enzyme.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast by enzymatic digestion of the cell wall. - [Read Preparation of cell lysates from yeast by enzymatic digestion of the cell wall]

Preparation of cell lysates from yeast using a French Press - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_yeast_press.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast using a French Press. he Protein Expression and Purification Facility, EMBL - [Read Preparation of cell lysates from yeast using a French Press]

Preparation of cell lysates from yeast using glass beads vortexing - http://www.embl.de/ExternalInfo/protein_unit/draft_frames/protocol_database/Purification/ec_glass_beads.html

Category: Protein Protocols: Protein Extraction From Cells

Preparation of cell lysates from yeast using glass beads vortexing. Includes lysis buffer recipes. The Protein Expression and Purification Facility, EMBL - [Read Preparation of cell lysates from yeast using glass beads vortexing]

Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of Intact DNA from Yeast - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3235

Category: Nucleic Acid Electrophoresis Protocols: Pulsed Field Gel Electrophoresis Protocols

Protocol for preparation of DNA for pulsed-field gel electrophoresis: isolation of intact DNA from yeast. Yeast cells are first treated enzymatically to break down the cell walls and then resuspended in low-melting-temperature agarose plugs. The DNA is liberated by infusing the plugs with lysis buffer and proteases. This method is used to prepare both conventional and artificial yeast chromosomes. - [Read Preparation of DNA for Pulsed-field Gel Electrophoresis: Isolation of Intact DNA from Yeast]

Preparation of Genomic DNA from Yeast Using Glass Beads Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4151

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the preparation of genomic DNA from a 5-ml liquid culture of yeast cells. - [Read Preparation of Genomic DNA from Yeast Using Glass Beads Protocol]

Preparation of Yeast Cells for Flow Cytometry Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4178

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Flow cytometry is used to analyze the quantity of DNA in cells. Since the DNA content of cells varies through the cell cycle, this information can provide an indication of cell cycle progression. This protocol uses SYTOX Green staining. - [Read Preparation of Yeast Cells for Flow Cytometry Protocol]

Preparation of Yeast DNA Embedded in Agarose Plugs Protocol - http://fangman-brewer.genetics.washington.edu/plug.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol for preparation of Yeast DNA embedded in agarose plugs. - [Read Preparation of Yeast DNA Embedded in Agarose Plugs Protocol]

Protcol for In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast - http://www.bio.com/protocolstools/protocol.jhtml?id=p1831

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol includes information on: Preparation of Membranes; One-Stage Reaction; Two-Stage Reaction using Normal Amount of Membranes; Two-Stage Reaction using Low Concentration of Membranes. - [Read Protcol for In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast]

Protein Extraction from Yeast Protocol - http://www.biochem.emory.edu/labs/acorbe2/yeastprotein.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Protocol for protein extraction from Yeast. - [Read Protein Extraction from Yeast Protocol]

Protein Immunoprecipitation from a Yeast Cell Lysate Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/yeastip.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Immunoprecipitation Protocols

Protocol for protein immunoprecipitation from a Yeast cell lysate. - [Read Protein Immunoprecipitation from a Yeast Cell Lysate Protocol]

Protocol Guide for the N. crassa Yeast Artificial Chromosome Library - http://www.fgsc.net/fgn41/yac1.html

Category: Fungi Protocols: Neurospora Protocols

Protocol guide for the N. crassa yeast artificial chromosome library. Includes: Chromosome Walking; Hybridization screening of the YAC library; YAC restriction mapping and contig building; Preparation of chromosomal DNA plugs of YAC clones; Partial restriction enzyme digestion of YAC DNA plugs; Using CHEF gel analysis to resolve YAC clones; Southern Hybridization; Isolation of terminal restriction fragments from cloned DNA inserts in YAC clones; etc. - [Read Protocol Guide for the N. crassa Yeast Artificial Chromosome Library]

Pulse-Chase Assay for Measuring Protein Stability in Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4641

Category: Yeast Protocols: Yeast Protein Protocols

This assay directly measures the stability of a specific protein in yeast. Following pulse-labeling of yeast cells, the target protein is recovered by denaturing immunoprecipitation. - [Read Pulse-Chase Assay for Measuring Protein Stability in Yeast Protocol]

Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4393

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol describes a method for purification of YAC DNA using ultrafiltration. This is the fastest method available, but care needs to be taken not to damage the DNA. The purified DNA can then be used for microinjection. - [Read Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol]

Quantitative Mating Assay Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/qma.html

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for quantitative mating assay. - [Read Quantitative Mating Assay Protocol]

Random Spore Analysis in Yeast Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4162

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

Random spore analysis is used to examine meiotic recombination without performing tetrad analysis. - [Read Random Spore Analysis in Yeast Protocol]

Rapid and Reliable Preparation of Total Cell Lysates from Fission Yeast Protocol - http://www.natureprotocols.com/2006/07/21/rapid_and_reliable_preparation.php

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Protocol for rapid and reliable preparation of total cell lysates from fission yeast. Method for successful and reproducible extraction of proteins from S. pombe cells. - [Read Rapid and Reliable Preparation of Total Cell Lysates from Fission Yeast Protocol]

Rapid Isolation of Yeast DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4039

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

This method is used to isolate genomic yeast DNA or shuttle plasmids that replicate in both S. cerevisiae and E. coli. The DNA can be used as a template for PCR and for transformation. - [Read Rapid Isolation of Yeast DNA Protocol]

Removal of Yeast Contamination from Lymphoblast Cultures Protocol - http://humgen.wustl.edu/hdk_lab_manual/hcc/hcc8.html

Category: Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols

This method is advantageous for saving the occasional cultures that become contaminated. Yeast contaminated cultures will appear cloudy when slightly shaken and lymphocytes will not cluster together as much as normal. If cultures are suspect, a drop of culture can be streaked on a YPD media plate to check for growth of yeast colonies, or a 5 ml sample can be taken to Barnes Diagnostic Center for identification of yeast strain. - [Read Removal of Yeast Contamination from Lymphoblast Cultures Protocol]

Restriction Digests of High Molecular Weight Yeast DNA Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast5.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for restriction digests of high molecular weight yeast DNA. - [Read Restriction Digests of High Molecular Weight Yeast DNA Protocol]

Restriction Digests of YACs in Agarose Plugs Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast6.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for restriction digests of YACs in agarose plugs. - [Read Restriction Digests of YACs in Agarose Plugs Protocol]

Restriction Endonuclease Digestion of DNA in Agarose Plugs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4031

Category: Nucleic Acid Electrophoresis Protocols: Pulsed Field Gel Electrophoresis Protocols

Protocol for restriction endonuclease digestion of DNA in agarose plugs. Genomic DNA isolated from mammalian, yeast, or bacterial cells can be digested with restriction endonucleases by incubating agarose plugs containing the DNA in the presence of the desired enzyme. After digestion, the DNA can be fractionated by pulsed-field gel electrophoresis and either isolated from the gel or analyzed by Southern Hybridization. - [Read Restriction Endonuclease Digestion of DNA in Agarose Plugs Protocol]

Screening YAC Libraries Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast7.html

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol for screening YAC libraries. - [Read Screening YAC Libraries Protocol]

Single Tube Confirmation Yeast PCR Protocol - http://sequence-www.stanford.edu/group/yeast_deletion_project/single_tube_protocol.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

Protocol for single tube confirmation yeast PCR. Includes: Clonal purification; Zymolyase treatment; PCR reactions; PCR conditions; Agarose gel electrophoresis. - [Read Single Tube Confirmation Yeast PCR Protocol]

Articles (1-5 of 5)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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