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Isolation of Plant Transcription Factors Using a Modified Yeast One-Hybrid System - http://www.plantmethods.com/content/2/1/3

Category: Plant Biology Protocols: Plant Genetics Protocols

The preparation of expressional cDNA libraries for use in the yeast two-hybrid system is quick and efficient when using the dedicated Clontech™ product, the MATCHMAKER Library Construction and Screening Kit 3. This kit employs SMART technology for the amplification of full-length cDNAs, in combination with cloning using homologous recombination. - [Read Isolation of Plant Transcription Factors Using a Modified Yeast One-Hybrid System]

Isolation of Plasmid DNA from Yeast Cells: A Ten-Minute Preparation - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4150

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol describes the purification release of plasmid DNA from yeast cells. The resulting plasmid DNA can be used to transform Escherichia coli or yeast. - [Read Isolation of Plasmid DNA from Yeast Cells: A Ten-Minute Preparation]

Isolation of Yeast Genomic DNA for Southern Blot Analysis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4149

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol (modified from Hoffman and Winston 1987) describes the purification of genomic DNA from yeast cells. - [Read Isolation of Yeast Genomic DNA for Southern Blot Analysis Protocol]

Isolation of Yeast Mitochondria in aPre-Formed Iodixanol Gradient - http://www.axis-shield.com/densityhome/optiprep/S27.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Mitochondria Isolation Protocols

A discontinuous gradient of iodixanol is used in which the crude mitochondrial fraction is layered beneath the preformed gradient. In this protocol the osmolality of the gradient is approx 600 mOsm and the crude mitochondrial fraction is loaded in 40% iodixanol rather than 50% iodixanol. - [Read Isolation of Yeast Mitochondria in aPre-Formed Iodixanol Gradient]

K. lactis Protein Expression Kit PDF - http://www.neb.com/nebecomm/ManualFiles/manualE1000.pdf

Category: Protein Protocols: Protein Expression Protocols

K. lactis Protein Expression Kit. Recombinant Protein Expression in Yeast. NEB Biolabs - [Read K. lactis Protein Expression Kit PDF]

Large-Scale Preparation of Agarose Plugs of Yeast DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4392

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol describes the use of agarose plugs for isolation of yeast artificial chromosome (YAC) DNA. The DNA can then be run on a pulsed-field gel and used for microinjection to produce transgenic mice. - [Read Large-Scale Preparation of Agarose Plugs of Yeast DNA Protocol]

Large-Scale Yeast Transformation Protocol - http://ygac.med.yale.edu/mtn/LS_transf_prot.stm

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for large-scale yeast transformation. Includes: Yeast Cell Preparation; Large Scale Transformation; To plate on solid medium; To select in liquid. - [Read Large-Scale Yeast Transformation Protocol]

LiOAc Yeast Transformation Protocol - http://proteome.wayne.edu/transform.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

LiOAc/TE, 40% PEG in LiOAc/TE. Transformation Method and Protocol for Yeast. Finley Lab, Wayne State. - [Read LiOAc Yeast Transformation Protocol]

LiOAc Yeast Transformation Protocol - http://proteome.wayne.edu/transform.html

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for LiOAc Yeast transformation. - [Read LiOAc Yeast Transformation Protocol]

Lipid Extraction from Plasma Membrane Fractions Prepared from Yeast Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p419

Category: Lipid Protocols

Protocol for lipid extraction from plasma membrane fractions prepared from yeast cells. - [Read Lipid Extraction from Plasma Membrane Fractions Prepared from Yeast Cells Protocol]

Lipid Extraction from Yeast Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p420

Category: Lipid Protocols

Protocol for lipid extraction from yeast cells. - [Read Lipid Extraction from Yeast Cells Protocol]

Lithium Acetate Transformation of Yeast Protocol - http://www.genomics.princeton.edu/dunham/yeasttransformation.htm

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for lithium acetate transformation of yeast. - [Read Lithium Acetate Transformation of Yeast Protocol]

Logarithmic Yeast Growth Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4179

Category: Yeast Protocols

Protocol for logarithmic Yeast growth. - [Read Logarithmic Yeast Growth Protocol]

Long Term Storage of Yeast Stocks Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast2.html

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

Yeast strains may be stored indefinitely at low temperatures (-80 degrees C). Two archiving methods are presented below. In Method A , the cells are grown on a plate, while in Method B the cells are grown in liquid culture. - [Read Long Term Storage of Yeast Stocks Protocol]

Measuring Volume Protocol - http://homepages.gac.edu/~cellab/chpts/chpt1/ex1-6.html

Category: Microscopy Protocols

Protocol for measuring volume. Material required includes: Microscope, Hemacytometer and coverslip, Suspension of yeast. - [Read Measuring Volume Protocol]

Measuring Yeast Cell Density by Spectrophotometry Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4186

Category: Yeast Protocols

Simple method for determining the cell density of yeast cultures. - [Read Measuring Yeast Cell Density by Spectrophotometry Protocol]

Media and Culture of Yeast Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=E5192D47CE9B0776DB7D136C34ED148A&objectid=6673ABA6C2DB2E4E7564EDFA26589256

Category: Yeast Cell Culture Protocols: Yeast Media, Solutions and Stocks Protocols

The yeast, Saccharyomyces cerevisiae, has become an important organism in molecular, biochemical, and genetic analysis. The organism has specific requirements for growth under a variety of conditions. The media, both liquid and solid, simple, define, and complex are describe in this unit. Also included are methods for handling, storing, and shipping stock of yeast. - [Read Media and Culture of Yeast Protocol]

Method to Restart Stuck Fermentation - http://www.vinquiry.com/pdf/METHODTORESTARTSTUCKFERMENTATION2003.pdf

Category: Bacterial Cell Culture Protocols: Fermentation Equipment

Method to restart stuck fermentation. Includes: Select and rehydrate active dry wine yeast; activate the rehydrated yeast with nutrients and sugar; start the fermentation and add the stock wine in batches. - [Read Method to Restart Stuck Fermentation]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Category: Yeast Protocols

Protocol for microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in Yeast. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport]

Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass - http://www.bio.com/protocolstools/protocol.jhtml?id=p1841

Category: Cell Organelle Protocols: Microsome Protocols

Protcol relating to microsome high speed pellet and cytosol preparation for endoplasmic reticulum to golgi transport assay in yeast. Includes spheroplast formation. - [Read Microsome High Speed Pellet and Cytosol Preparation for Endoplasmic Reticulum to Golgi Transport Ass]

Microsome Preparation from Yeast Cells Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1676

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Microsome Isolation Protocols

This protocol describes the isolation of microsomal Endoplasmic Reticulum (ER) membranes from yeast cells. Includes: Spheroplast Formation and Microsome Purification. - [Read Microsome Preparation from Yeast Cells Protocol]

No Boil Yeast Extracts Protocol - http://www.ciwemb.edu/labs/koshland/Protocols/Protein/noboil.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Protocol for no boil Yeast extracts. - [Read No Boil Yeast Extracts Protocol]

nup49-313 Shuttling in Yeast Protocol - http://www.biochem.emory.edu/labs/acorbe2/nup49.html

Category: Yeast Protocols: Yeast Staining Protocols

Protocol for nup49-313 shuttling in Yeast. - [Read nup49-313 Shuttling in Yeast Protocol]

PCR-Mediated Gene Disruption: One-Step Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4169

Category: PCR Protocols

DNA prepared by PCR-mediated gene disruption can be used to transform yeast in gene replacement experiments. This protocol uses two primers, tailed with approximately 50 nucleotides homologous to the gene of interest, that target insertion of the PCR product to that locus. Each primer ends with a universal sequence that is designed to amplify various selectable markers from plasmid templates. - [Read PCR-Mediated Gene Disruption: One-Step Method Protocol]

Phenol/Freeze RNA Prep Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/rprep.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast RNA Isolation Protocols

Protocol for phenol/freeze RNA prep. - [Read Phenol/Freeze RNA Prep Protocol]

Articles (1-5 of 5)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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