User Panel

My Panel

Bookmark Science Articles

yeast Protocols

Search results for: yeast

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

GST Fusion Protein Purification from Yeast - http://www.med.unc.edu/~hdohlman/gst.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

GST Fusion Protein Purification from Yeast. Lysis Buffer recipe for yeast, glutathione bead method for GST purification. Dohlman Lab - [Read GST Fusion Protein Purification from Yeast]

GST Fusion Protein Purification from Yeast Protocol - http://www.med.unc.edu/~hdohlman/gst.html

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Protein Purification and Isolation Protocols

Protocol for GST fusion protein purification from Yeast. - [Read GST Fusion Protein Purification from Yeast Protocol]

HC Dropout Plate Supplementation Table - http://www.fhcrc.org/science/labs/gottschling/yeast/ysup.html

Category: Yeast Cell Culture Protocols: Yeast Sporulation Protocols

HC Dropout Plate Supplementation Table. - [Read HC Dropout Plate Supplementation Table]

High Efficiency LiAc Transformation Protocol - http://www.fhcrc.org/science/labs/gottschling/yeast/ytrans.html

Category: Yeast Protocols: Yeast Transformation Protocols: High Efficiency Yeast Transformation Protocols

Protocol for high efficiency LiAc transformation. - [Read High Efficiency LiAc Transformation Protocol]

High Molecular Weight Yeast Liquid DNA Preparation Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast4.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast DNA Isolation Protocols

Protocol that isolates intact, high molecular weight DNA from yeast cells for subcloning and rare cutting restriction enzyme analysis. Using this protocol one can expect a yield of 100-200 µg of DNA per prep. - [Read High Molecular Weight Yeast Liquid DNA Preparation Protocol]

High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol - http://www.natureprotocols.com/2006/11/22/highthroughput_and_sensitive_a.php

Category: Pharmacology and Toxicology Protocols: Drug Discovery

High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents. Method is highly sensitive, provides quantifiable data and offers high-throughput screening capability. Starting with the treatment of cells with different doses of damaging agents, pre-prepared growing media containing 96-well plates are inoculated and cell population is automatically monitored every 10 min for 48 hours. - [Read High Throughput and Sensitive Assay Measure Yeast Cell Growth Protocol]

High Yield Protein Production from Pichia pastoris Yeast PDF - http://www.nbsc.com/files/papers/abl_pichia.pdf

Category: Protein Protocols: Protein Expression Protocols

High Yield Protein Production from Pichia pastoris Yeast PDF. Julia Cino, PhD - [Read High Yield Protein Production from Pichia pastoris Yeast PDF]

High-Efficiency Transformation of Yeast Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4145

Category: Yeast Protocols: Yeast Transformation Protocols: High Efficiency Yeast Transformation Protocols

Protocol for high-efficiency transformation of Yeast. - [Read High-Efficiency Transformation of Yeast Protocol]

Hybridization of Arrays - http://cmgm.stanford.edu/pbrown/protocols/5_hyb_yeast.html

Category: DNA Microarray Protocols

Hybridization of Arrays. . General/Yeast Microarrays. Brown Lab - [Read Hybridization of Arrays]

Hydroxylamine Mutagenesis of Plasmid DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4156

Category: Yeast Protocols: Yeast Nucleic Acid Protocols

Protocol produces mutagenized DNA that can be used for transformation of either Escherichia coli or yeast. - [Read Hydroxylamine Mutagenesis of Plasmid DNA Protocol]

Identification of End Clones in YAC Subclone Libraries Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast10.html

Category: Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols

To identify the YAC subclones containing both a human insert and a portion of either the left or right arm of the pYAC4 vector. Identification of these clones is necessary in order to do YAC chromosome walking, and is also useful in the determination of whether a particular YAC clone has a contiguous human insert or whether a co-cloning event has occurred. Vector arm sequences are identified using pBR322 fragments from a BamHI-PvuII double digest. - [Read Identification of End Clones in YAC Subclone Libraries Protocol]

Immunoblotting: Preparing Cell Lysates Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4299

Category: Western Blot Protocols: General Western Blot Methods Protocols

For immunoblotting experiments, it is often important to compare the total amount of an antigen from many different sources or to learn if a particular source has the antigen under study. In the approach described here, tissue cultures, bacteria, yeast cells, tissues, and other sources of antigens are disrupted directly in an electrophoresis sample . - [Read Immunoblotting: Preparing Cell Lysates Protocol]

Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF - http://derisilab.ucsf.edu/pdfs/BeadBeatIP_Protocol.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Chromatin-IP (or ChIP) has been a useful method for the localization of proteins to specific regions of DNA. ChIP applied to microarrays (ChIP on Chip) Brown Lab - [Read Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF]

Immunoprecipitation of Epitope-Tagged Proteins from Yeast Protocol - http://www.med.unc.edu/~hdohlman/TAP.html

Category: Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols

Protocol for immunoprecipitation of epitope-tagged proteins from yeast. - [Read Immunoprecipitation of Epitope-Tagged Proteins from Yeast Protocol]

In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1831

Category: Yeast Protocols

Protocol for in vitro endoplasmic reticulum to golgi transport reaction in Yeast. Includes: Preparation of Membranes; One-Stage Reaction; Two-Stage Reaction using Normal Amount of Membranes; Two-Stage Reaction using Low Concentration of Membranes. - [Read In Vitro Endoplasmic Reticulum to Golgi Transport Reaction in Yeast Protocol]

In Vitro Transcription With Yeast Nuclear Extract - http://www.fhcrc.org/science/labs/hahn/methods/biochem_meth/polii_trans.html

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription with yeast nuclear extract. Includes recipes for: 5x Acetate transcription buffer; Creatine phospho kinase; Phospho creatine; Stop mix; HA + 0.1 M potassium acetate; 5x glutamate transcription buffer (5 ml). Includes also protocol for Primer Extension Assay. - [Read In Vitro Transcription With Yeast Nuclear Extract]

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4173

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

It is sometimes desirable to have an entire population of yeast cells that is growing synchronously or is arrested at a unique point in the cell cycle. - [Read Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using bar1 Mutants Protocol]

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using Low Cell Concentration Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4171

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: alpha-Factor Arrest Using Low pH Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4172

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: Dilution of Stationary-Phase Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4176

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: Hydroxyurea Arrest Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4174

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Inducing Yeast Cell Synchrony: Nocodazole Arrest Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4175

Category: Yeast Protocols: Yeast Cell Synchrony Protocols

Introduction to the Genetics and Molecular Biology of the Yeast Saccharomyces cerevisiae - http://dbb.urmc.rochester.edu/labs/sherman_f/yeast/index.html

Category: Yeast Protocols: Yeast Genetics Protocols

Introduction of the of genetics and molecular biology of the Yeast Saccharomyces cerevisiae. - [Read Introduction to the Genetics and Molecular Biology of the Yeast Saccharomyces cerevisiae]

Isolation of Arabidopsis cDNAs by Complementation in Yeast - http://www.arabidopsis.org/cshl-course/12-yeast.html

Category: Plant Biology Protocols: Arabidopsis Nucleic Acid Protocols

Includes: Isolation of Arabidopsis LEU2 cDNAs by complementation of the yeast leu2 mutation; Recovery of plasmid DNA from yeast cells; Analysis of Arabidopsis cDNAs that complement a yeast leu2 mutation; Preparation of library DNA from the Lacroute cDNA library; Preparation of Yeast Media. - [Read Isolation of Arabidopsis cDNAs by Complementation in Yeast]

Isolation of High-molecular-weight DNA from Mammalian Cells Using Proteinase K and Phenol Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4036

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Isolation from Mammalian Cells Protocols

Method of choice when large amounts of mammalian DNA are required, for example, for Southern blotting (Rapid Isolation of Mammalian DNA, Rapid Isolation of Yeast DNA, Southern Blotting: Capillary Transfer of DNA to Membranes) or for construction of genomic libraries in bacteriophage {lambda} vectors. Approximately 200 µg of mammalian DNA, 100-150 kb in length, is obtained from 5 x 107 cultured aneuploid mammalian cells (e.g., HeLa cells). - [Read Isolation of High-molecular-weight DNA from Mammalian Cells Using Proteinase K and Phenol Protocol]

Articles (1-5 of 5)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

[1-5]

Items 51-75 out of 187 displayed.

First Previous 1 2 [3] 4 5 6 7 8 Next Last

Total records: 187