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A Triple Color FISH Technique for Mouse Chromosome Identification - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html

Category: BACs Bacterial Artificial Chromosome Protocols

Describe a set of BACs that map close to the centromeric and distal chromosomal ends for each mouse chromosome. - [Read A Triple Color FISH Technique for Mouse Chromosome Identification]

Affymetrix Real Time PCR information and optimization - http://keck.med.yale.edu/affymetrix/rtpcr/

Category: Real Time PCR

Affymetrix Real Time PCR information and optimization. Yale U. Keck Foundation. - [Read Affymetrix Real Time PCR information and optimization]

Alexa Dyes, Cy Dyes and Other Fluors: Succinimidyl Esters - http://info.med.yale.edu/genetics/ward/tavi/FISHdyes.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Alexa Dyes, Cy Dyes and other Fluors: succinimidyl esters. - [Read Alexa Dyes, Cy Dyes and Other Fluors: Succinimidyl Esters]

Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative - http://info.med.yale.edu/genetics/ward/tavi/CCKmethod.html

Category: Cytogenetics Protocols

Protocol for color changing karytoping (CCK): an M-FISH/SKY alternative. Includes: Slide preparation and hybridization; Probes, dyes and antibodies; Probe detection and image capture; Image processing. - [Read Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative]

Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/TMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for cryptic translocation identification in human and mouse using several telomeric multiplex FISH (TM-FISH) strategies. - [Read Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH]

Curing Strains of Endogenous 2-Micron Plasmid Protocol - http://ygac.med.yale.edu/mtn/reagent/avail_reagents/curing2micron_info.stm

Category: Yeast Protocols: Yeast Genetics Protocols

Protocol for curing strains of endogenous 2-micron plasmid. - [Read Curing Strains of Endogenous 2-Micron Plasmid Protocol]

Custom Fluorescent Nucleotide Synthesis/Nucleic Acid Labeling Protocol - http://info.med.yale.edu/genetics/ward/tavi/n_coupling.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for custom fluorescent nucleotide synthesis/nucleic acid labeling. - [Read Custom Fluorescent Nucleotide Synthesis/Nucleic Acid Labeling Protocol]

Electron Microscopy Protocols Yale - http://cellserv.med.yale.edu/imaging/ccmi/elect_protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Protocol 2 - Embedding of cell monolayer in epoxy resin. Protocol 3 - Embedding of scraped cells ... Welcome to the new CCMI: Electron Microscopy Website. ... Yale Protocols. - [Read Electron Microscopy Protocols Yale]

FISH Guide and Troubleshooting - http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

G-Banding Observations - http://info.med.yale.edu/genetics/ward/tavi/slide08.html

Category: Cytogenetics Protocols

G-Banding observations. Includes: Slide storage; Fixative composition and trypsin treatment in G-banding; Banding of chemically aged slides. - [Read G-Banding Observations]

Handling and Using Insertion Libraries - http://ygac.med.yale.edu/mtn/protocol.stm

Category: Yeast Protocols

Handling and using insertion libraries. Includes: Curing strains of endogenous 2-micron; mTn-lacZ/LEU2 insertion library; mTn-3xHA/lacZ insertion library; mTn-3xHA/GFP insertion library. - [Read Handling and Using Insertion Libraries]

Large-Scale Immunocytology Protocol - http://ygac.med.yale.edu/mtn/Immu_protocols.stm

Category: Yeast Protocols: Yeast Staining Protocols

Protocol describes our method for preparing cells for immunofluorescence, in which all incubations and washes are performed in microtiter dishes. The protocol can also easily be adapted for preparing cells for immunofluorescence in microfuge tubes. - [Read Large-Scale Immunocytology Protocol]

Large-Scale Yeast Transformation Protocol - http://ygac.med.yale.edu/mtn/LS_transf_prot.stm

Category: Yeast Protocols: Yeast Transformation Protocols

Protocol for large-scale yeast transformation. Includes: Yeast Cell Preparation; Large Scale Transformation; To plate on solid medium; To select in liquid. - [Read Large-Scale Yeast Transformation Protocol]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: PCR Protocols: PCR Optimization

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: PCR Protocols: Standard PCR Protocol

PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: PCR Buffer Protocols

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: Standard PCR Protocol

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

PCR Program Design - http://info.med.yale.edu/genetics/ward/tavi/p08.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

The requirement of an optimal PCR reaction is to amplify a specific locus without any unspecific by-products. Therefore, annealing needs to take place at a sufficiently high temperature to allow only the perfect DNA-DNA matches to occur in the reaction. P - [Read PCR Program Design]

PCR Program Design - http://info.med.yale.edu/genetics/ward/tavi/p08.html

Category: PCR Protocols: PCR Optimization

Processing Adherent Cultured Cells for Tissue Microarray Controls ... - http://www.tissuearray.org/CellLinesProtocolforTMA112.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Cell Fixation. 1. Gently wash cells 2x in sterile 1XPBS, ... [Take to Research Histology for embedding] Instructions: Embed pellets in vertical ... Jena Giltnane Yale University Dept. of Pathology. Processing Adherent Cultured Cells for Tissue Microarray Controls. - [Read Processing Adherent Cultured Cells for Tissue Microarray Controls ...]

Real-time/SNP ABI 7900HT Protocol PDF. :Real Time PCR - http://keck.med.yale.edu/affymetrix/protocols/Real%20time.pdf

Category: Real Time PCR

Real-time/SNP ABI 7900HT Protocol PDF. :Real Time PCR - [Read Real-time/SNP ABI 7900HT Protocol PDF. :Real Time PCR]

Slide Preparation for Cytogenetics Protocol - http://info.med.yale.edu/genetics/ward/tavi/Cytom-slideprep.html

Category: Cytogenetics Protocols

Protocol for slide preparation for cytogenetics. Includes: Improved cell recovery by microcentrifugation; Slide storage; "Controlled" chromosome spreading; Dropping cells from a height does not improve spreading; GTG-banding. - [Read Slide Preparation for Cytogenetics Protocol]

Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/CMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH). This approach allows the identification of marker chromosomes and other aneuploides on one single cytogenetic slide, in less than two hours. - [Read Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH]

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