yacs Protocols

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Protocol describes the use of vectorette PCR and single-site PCR to amplify the terminal sequences of genomic sequences cloned in high-capacity vectors such as PACs and YACs. - [Read Isolating the Ends of Genomic DNA Fragments Cloned in High-capacity Vectors]

Restriction Digests of YACs in Agarose Plugs Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast6.html

Protocol for restriction digests of YACs in agarose plugs. - [Read Restriction Digests of YACs in Agarose Plugs Protocol]

Working with Yeast Artificial Chromosomes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3297

Protocol outlines a procedure to validate and store yeast strains carrying YACs. - [Read Working with Yeast Artificial Chromosomes Protocol]

Yeast DNA Isolation Protocol for FISH - http://www.sanger.ac.uk/HGP/methods/cytogenetics/yacs.shtml

Yeast DNA Isolation Protocol for FISH. Sanger Institute. - [Read Yeast DNA Isolation Protocol for FISH]

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