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Cartilage Staining of Xenopus tropicalis Protocol - http://tropicalis.berkeley.edu/home/gene_expression/cartilage-stain/alcian.html

Category: Xenopus Protocols

Protocol describes the use of a basic water-based dye to stain for acid mucosubstances and acetic mucins located on the cartilage of fixed embryos, permitting the examination of cartilage formation. - [Read Cartilage Staining of Xenopus tropicalis Protocol]

CSF Extract Prep for Xenopus Spindle Assembly Protocol - http://mitchison.med.harvard.edu/protocols/ext3.html

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for CSF extract prep for Xenopus spindle assembly. - [Read CSF Extract Prep for Xenopus Spindle Assembly Protocol]

Cytostatic Factor (CSF) Extract Preparation for Xenopus Spindle Assembly Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1422

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol for cytostatic factor extract preparation for spindle assembly. The quality of the eggs is essential for good CSF extracts. Always sacrifice quantity for quality when trying to make functional extracts. Discard any batches of eggs that have "puff balls" or activated eggs that constitute more than 10% of the eggs. Use laid eggs and collect eggs at about 16 to 17 hours after priming with Progesterone (found in Pregnant Mare Serum Gonadotropin (PMSG)). - [Read Cytostatic Factor (CSF) Extract Preparation for Xenopus Spindle Assembly Protocol]

Diseases of Xenopus tropicalis - http://tropicalis.berkeley.edu/home/husbandry/disease.html

Category: Xenopus Protocols

Information on the diseases of Xenopus tropicalis. Includes: PREMETAMORPHS: Bacterial Infection of Embryos; Protozoal Infestation of Tadpoles; POSTMETAMORPHS: Mycobacteriosis; Chytridiomycosis (Batrachochytrium dendrobatidis). - [Read Diseases of Xenopus tropicalis]

ELISA for Detection of Xenopus Antibodies Against FV3 Protocol - http://www.urmc.rochester.edu/smd/mbi/xenopus/Protocols/FV3ELISA.pdf

Category: ELISA Protocols

Protocol for ELISA for detection of Xenopus antibodies against FV3. - [Read ELISA for Detection of Xenopus Antibodies Against FV3 Protocol]

ELISA for Detection of Xenopus Antibodies Against FV3 Protocol - http://www.urmc.rochester.edu/smd/mbi/xenopus/Protocols/FV3ELISA.pdf

Category: Xenopus Protocols

Protocol for ELISA for detection of Xenopus antibodies against FV3. - [Read ELISA for Detection of Xenopus Antibodies Against FV3 Protocol]

Fixation of Xenopus Embryos - http://tropicalis.berkeley.edu/home/manipulate_embryos/fixation.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for the fixation of xenopus embryos. - [Read Fixation of Xenopus Embryos]

Histone H1 Kinase Activity Assay - http://www.bio.com/protocolstools/protocol.jhtml?id=p910

Category: Signalling Signal Transduction Protocols

This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography. Includes information: H1 Kinase Assay on Individual Xenopus Oocytes; H1 Kinase Assay on Xenopus Egg Extract Samples; H1 Kinase Assay on Tissue Culture Cells; Helpful protocol hints. - [Read Histone H1 Kinase Activity Assay]

Isolating Xenopus Genomic DNA Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/genomicDNA.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for isolating Xenopus genomic DNA. - [Read Isolating Xenopus Genomic DNA Protocol]

Karyotyping Xenopus Embryos Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/karyotype.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for karyotyping Xenopus embryos. - [Read Karyotyping Xenopus Embryos Protocol]

Kinase Assay Using In Vitro Translated Xenopus Mos Kinase - http://www.bio.com/protocolstools/protocol.jhtml?id=p912

Category: Signalling Signal Transduction Protocols

Protocol describes how tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay. Protocol includes: Translation of Xenopus Mos Kinase; Antibody to Antigen Binding; Protein A Sepharose to Antibody Binding; Kinase Reactions on Immunoprecipitated Material; Polyacrylamide Gel Analysis of Immunoprecipitates. Includes protocol hints. - [Read Kinase Assay Using In Vitro Translated Xenopus Mos Kinase]

Making and Injecting Xenopus Embryos Protocol - http://spot.colorado.edu/~klym/Methods/embryos.htm

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for making and injecting Xenopus embryos. Includes: Injections; Egg Activation and Inversion Experiments; - [Read Making and Injecting Xenopus Embryos Protocol]

Natural and Artificial Fertilization of Xenopus Eggs Protocol - http://www.hhmi.ucla.edu/derobertis/protocol_page/Pdfs/Natural%20and%20Artificial%20Fertilization%20of%20Frog%20Eggs.pdf

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for natural and artificial fertilization of Xenopus eggs. - [Read Natural and Artificial Fertilization of Xenopus Eggs Protocol]

Preparation of Xenopus Sperm Nuclei Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p877

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol describes the preparation of nuclei from Xenopus sperm. These nuclei can be used for spindle assembly assays, microscopy, or DNA synthesis. - [Read Preparation of Xenopus Sperm Nuclei Protocol]

Priming and Inducing Ovulation in Xenopus Protocol - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3DO5QPPHSY0GZ31R3FQLMSFEWHUWBNQIV0?id=p9027

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol for priming and inducing ovulation in Xenopus. - [Read Priming and Inducing Ovulation in Xenopus Protocol]

Protocol Spindle Assembly In Vitro - http://www.bio.com/protocolstools/protocol.jhtml?id=p1051

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol describes an in vitro reaction to assay mitotic spindle assembly. The assay uses Cytostatic Factor extract made from Xenopus eggs, fluorescently-labelled tubulin, and prepared sperm nuclei. Spindle assembly is monitored by immunofluorescence microscopy. - [Read Protocol Spindle Assembly In Vitro]

Screening cDNA Libraries Constructed in Eukaryotic Expression Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4069

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Two methods of screening pools of cloned cDNAs are discussed: transfection into mammalian cells and injection into Xenopus oocytes. - [Read Screening cDNA Libraries Constructed in Eukaryotic Expression Vectors Protocol]

TUNEL Staining Protocol for Xenopus - http://tropicalis.berkeley.edu/home/gene_expression/TUNEL/TUNEL.html

Category: Xenopus Protocols: Xenopus Embryo Manipulation Protocols

Protocol is effective in identifying apoptotic cells in embryos that are undergoing substantial apoptosis. - [Read TUNEL Staining Protocol for Xenopus]

Xenopus cDNA Libraries - http://www.xenbase.org/other/static/methods/cDNA_libraries.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Xenopus cDNA libraries. Includes: General cDNA clone providers. - [Read Xenopus cDNA Libraries]

Xenopus Chromatin immmunoprecipitation Protocol - http://spot.colorado.edu/~klym/Methods/XChIP.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus chromatin immunoprecipitation. - [Read Xenopus Chromatin immmunoprecipitation Protocol]

Xenopus DNA Fishing Protocol - http://spot.colorado.edu/~klym/Methods/fishing.htm

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus DNA fishing. Includes: DNA-agarose coupling reaction; PROTEIN BINDING REACTION; OLIGO ANNEALING. - [Read Xenopus DNA Fishing Protocol]

Xenopus Egg CSF Extract Preparation Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1049

Category: Xenopus Protocols: Xenopus Egg Extracts Protocols

Protocol describes the preparation of Cytostatic Factor (CSF) from frog eggs. CSF can be used in in vitro spindle assembly assays. Includes: Frog injections; Preparation Before Starting the Extract Preparation; Extract Preparation. - [Read Xenopus Egg CSF Extract Preparation Protocol]

Xenopus Fluorescent in situs and FCIS Protocol - http://www.xenbase.org/other/static/methods/FISH.jsp

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocols for performing wholemount fluorescent in situs. Instructions for two-color FISH and for a combination of fluorescent and colorimetric in situs we have called FCIS. Data can be imaged with a fluorescence stereoscope or a confocal. - [Read Xenopus Fluorescent in situs and FCIS Protocol]

Xenopus Gynogenesis Protocol - http://tropicalis.berkeley.edu/home/genetic_techniques/gynogenesis/gynogenesis.html

Category: Xenopus Protocols: Xenopus Genetics Protocols

Protocol for Xenopus gynogenesis. - [Read Xenopus Gynogenesis Protocol]

Xenopus Microinjection Protocol - http://tropicalis.berkeley.edu/home/manipulate_embryos/microinjection/inject.html

Category: Xenopus Protocols: Xenopus Injection Protocols

Protocol for Xenopus microinjection. - [Read Xenopus Microinjection Protocol]

Articles (1-2 of 2)

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

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