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Directed Yeast Two Hybrid Screening for MEKK1 Interaction Partners Protocol - http://www.natureprotocols.com/2007/01/22/directed_yeast_two_hybrid_scre.php

Category: Yeast Protocols: Yeast Two-Hybrid Systems Protocols

Protocol utilizes the Y190 yeast strain, that characteristically turns from white to red during growth due to ademutation. Baits were subcloned into the pAS1cyh2 vector, and Preys subcloned into pACT2. - [Read Directed Yeast Two Hybrid Screening for MEKK1 Interaction Partners Protocol]

Light and Electron Microscopy Detection of Apoptosis - http://www.jhc.org/cgi/content/full/47/4/561

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Detection of Fragmented DNA in Apoptotic Cells Embedded in LR White:Histochemical (LM) and Ultrastructural (EM). Goping et al 1999. - [Read Light and Electron Microscopy Detection of Apoptosis]

TEM Sample Preparation Links - http://bama.ua.edu/~hsmithso/class/bsc_656/websites/sample_tem.html

Category: Molecular Imaging: Electron Microscopy Protocols

Embedding. Diagnostic Electron Microscopy on Reembedded ("Popped Off") Areas of Large Spurr Epoxy Sections ï¿½ LR White Microwave Processing Protocol . TEM Sample Preparation Links and Protocols. - [Read TEM Sample Preparation Links]

Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html

Category: DNA Protocols: TOPO Cloning

Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]