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Affinity purification of antibodies from crude serum - http://wheat.pw.usda.gov/~lazo/methods/au/sect32.html

Category: Protein Protocols: Antibody Purification Protocols

Affinity purification of antibodies from crude serum. David Bowtell Lab. - [Read Affinity purification of antibodies from crude serum]

Agrobacterium-Mediated Gene Transfer via Hypocotyls Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/cottontr.html

Category: Plant Biology Protocols: Plant Genetics Protocols

Protocol describing Agrobacterium mediated gene transfer via hypocotyls. - [Read Agrobacterium-Mediated Gene Transfer via Hypocotyls Protocol]

Antibiotic Concentration in Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/antibiot.html

Category: Buffers Media, and Solutions: Bacterial Solutions

Antibiotic Concentration in Media. (Gerard R. Lazo) - [Read Antibiotic Concentration in Media]

Arabidopsis Thaliana DNA Isolation Procedure - http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdna.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Procedure to isolate DNA from Arabidopsis Thaliana. - [Read Arabidopsis Thaliana DNA Isolation Procedure]

Arabidopsis thaliana DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdna.html

Category: Plant Biology Protocols: Arabidopsis Nucleic Acid Protocols

Protocol for Arabidopsis thaliana DNA isolation procedure. - [Read Arabidopsis thaliana DNA Isolation Protocol]

Arabidopsis Tissue Culture Stock Solutions and Media - http://wheat.pw.usda.gov/~lazo/methods/lazo/tissue.html

Category: Plant Biology Protocols: Arabidopsis Tissue Culture

Arabidopsis Tissue Culture Stock Solutions and Media. Lazo Lab. MS MEDIUM FOR ARABIDOPSIS. B5 MEDIUM FOR ARABIDOPSIS. 10X Micronutrients. - [Read Arabidopsis Tissue Culture Stock Solutions and Media]

Chloroplast Isolation DNA Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplchl.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol for the isolation of DNA from the chloroplast. - [Read Chloroplast Isolation DNA Protocol]

Collection and Storage of Cotton Leaf Tissue - http://wheat.pw.usda.gov/~lazo/methods/lazo/collect.html

Category: Plant Biology Protocols

Protocol for the collection and storage of cotton leaf tissue. - [Read Collection and Storage of Cotton Leaf Tissue]

Common Buffers - http://wheat.pw.usda.gov/~lazo/methods/lazo/buffer.html

Category: Buffers Media, and Solutions

Recipes for commonly used buffer solutions. (Gerard R. Lazo) - [Read Common Buffers]

Confocal Microscopy Specimen Preparation Using Synthetic Fluorophores and Immunofluorescence - http://www.olympusconfocal.com/applications/protocols/index.html

Category: Microscopy Protocols: Confocal Microscopy Protocols

Includes staining protocols: Triple-Staining Adherent Cells with MitoTracker, Phalloidin,& Nuclear Dyes; Staining Adherent Cells with Cytokeratin Primary Antibodies & Synthetic Fluorophores; Staining Adherent Cells with Intermediate Filament Primary Antibodies & Synthetic Fluorophores; Staining Cells & Tissue Cryosections with Tubulin Primary Antibodies, Phallotoxins,& Synthetic Fluorophores; Triple-Staining Tissue Cryosections with Wheat Germ Agglutinin, Phalloidin,& Nuclear Dyes; etc. - [Read Confocal Microscopy Specimen Preparation Using Synthetic Fluorophores and Immunofluorescence]

Differential cDNA Screening Procedures - http://wheat.pw.usda.gov/~lazo/methods/misc/Talbot.html

Category: RNA Protocols: cDNA Libraries Library

Differential cDNA Screening Procedures. The protocols listed refer to cDNA library construction and preliminary differential screening procedures. Nick Talbot lab - [Read Differential cDNA Screening Procedures]

DNA Extraction Procedure (CsCl) - Rhizobium - http://wheat.pw.usda.gov/~lazo/methods/lazo/rhizodna.html

Category: Plant Biology Protocols: Plant DNA RNA Isolation Protocols

Protocol describing extraction of DNA from Rhizobium. - [Read DNA Extraction Procedure (CsCl) - Rhizobium]

Drosophila Single-Fly DNA Preps For PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafly.html

Category: Drosophila Protocols: Drosophila Nucleic Acids Protocols

Protocol for Drosophila single-fly DNA preps for PCR. Includes: DNA Preparation Protocol; DNA Preps from many individual flies; Inverse PCR. - [Read Drosophila Single-Fly DNA Preps For PCR Protocol]

ELISA Inhibition Assay - http://wheat.pw.usda.gov/~lazo/methods/goldberg/elisa.html

Category: ELISA Protocols

Protocol and method for ELISA Inhibition Assay. Michael Coyne. Goldberg Lab - [Read ELISA Inhibition Assay]

Fungal DNA Isolation Method - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: DNA Protocols: DNA Extraction Protocols

DNA isolation from various fungal species including: Cochliobolus, Aternaria, and Fusarium. Key steps: (1) the use of young lyophilized mycelial mats - yield less contaminating carbohydrates; (2) proteinase K in the extraction buffer to destroy DNases (f - [Read Fungal DNA Isolation Method]

Fungal DNA Isolation Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/dnafun.html

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols

Protocol for fungal DNA isolation. The key elements in this prep are (1) the use of young lyophilized mycelial mats....young mats (4 days growth for C. carbonum)...yield less contaminating carbohydrates and other misc. junk (2) lots of proteinase K in the extraction buffer to kill Dnases (final =0.3mg/ml). - [Read Fungal DNA Isolation Protocol]

Immunodot Assay Protocol - http://wheat.pw.usda.gov/~lazo/methods/goldberg/immuno.html

Category: ELISA Protocols

Protocol for immunodot assay. - [Read Immunodot Assay Protocol]

In-Situ PCR Protocol - http://wheat.pw.usda.gov/~lazo/methods/minn/chapter3.7/chapter3.7.fm.html

Category: PCR Protocols: In-situ PCR Protocols

Amplification and Detection in a Cellular Context. Methodology, in-situ detection methods, reaction Conditions, introduction. Ernest F. Retzel et al., University of Minnesota. - [Read In-Situ PCR Protocol]

Isolation of poly A+ RNA from Total RNA by Oligo(deoxythymidine)cellulose Chromatography - http://wheat.pw.usda.gov/~lazo/methods/au/m6e1.html

Category: RNA Protocols: mRNA Extraction Protocols

Isolation of poly A+ RNA from Total RNA by Oligo(deoxythymidine)cellulose Chromatography. Total RNA is first isolated from the tissues or cells and then mRNA is isolated by PolyA+ selection using oligo(dT) cellulose. This is necessary for all tissue sources rich in RNase (and cell lines). Lazo Lab - [Read Isolation of poly A+ RNA from Total RNA by Oligo(deoxythymidine)cellulose Chromatography]

Isolation of poly A+ RNA from Total RNA by Oligo(deoxythymidine)cellulose Chromatography - http://wheat.pw.usda.gov/~lazo/methods/au/m6e1.html

Category: Chromatography Protocols: DNA RNA Affinity Chromatography

Isolation of poly A+ RNA from Total RNA by oligo(deoxythymidine)cellulose Chromatography Protocol. Faulkner-Jones - [Read Isolation of poly A+ RNA from Total RNA by Oligo(deoxythymidine)cellulose Chromatography]

LB Agar 200ml/1000ml Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/stocksgl.html

Category: Bacterial Cell Culture Protocols: Bacterial Cell Culture Media Protocols: Agar Cell Culture Media Protocols

Protocol for LB Agar 200ml/100ml. - [Read LB Agar 200ml/1000ml Protocol]

Lectin-Agarose Affinity Chromatography Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4202

Category: Chromatography Protocols: Affinity Chromatography Protocols

Two methods are provided for purifying glycoproteins using wheat-germ agglutinin or concanavalin A-Sepharose. Because lectin-affinity matrices can bind a few milligrams of protein per milliliter of affinity matrix, only a small amount of affinity gel matrix is required. The batchwise method is recommended when protein volume is large. - [Read Lectin-Agarose Affinity Chromatography Protocol]

Long-PCR Reagents and Guidelines - http://wheat.pw.usda.gov/~lazo/methods/lazo/longpcr.html

Category: PCR Protocols: Quantitative PCR Protocols

General guidelines for long-PCR conditions and enzyme mixtures. Efficient long-PCR results from the use of two polymerases: a non-proofreading polymerase is the main polymerase in the reaction, and a proofreading polymerase (3' to 5' exo) is present at a lower concentration. Includes: For PCR with low-complexity templates (e.g., plasmid and cosmid inserts); For PCR with moderate-complexity templates (e.g., bacterial genomic DNA); For PCR with high-complexity templates (e.g., human genomic DNA). - [Read Long-PCR Reagents and Guidelines]

Lyophilization of Cotton Leaves - http://wheat.pw.usda.gov/~lazo/methods/lazo/lyophili.html

Category: Plant Biology Protocols

Protocol for the lyophilization of cotton leaves. - [Read Lyophilization of Cotton Leaves]

Maintenance of Probes in Bacteria Including Escherichia coli Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/bactstor.html

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on Luria-Bertani (LB) agar on or in LB broth containing Ampicillin (30 µg/ml) or Carbenicillin (50 µg/ml broth, 100 µg/ml agar). E. coli strains are usually preserved in stab agar or glycerol for mid-term storage and lyophilized for long-term storage. - [Read Maintenance of Probes in Bacteria Including Escherichia coli Protocol]

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