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Replication Timing by Density Transfer Protocol - http://fangman-brewer.genetics.washington.edu/density_transfer.html

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing by density transfer. - [Read Replication Timing by Density Transfer Protocol]

Replication Timing Using Transient Hemimethylation Protocol - http://fangman-brewer.genetics.washington.edu/hemimethylation.html

Category: Yeast Protocols: Yeast Genetics Protocols: Yeast Replication Protocols

Protocol for replication timing using transient hemimethylation. Includes: Strain construction; Protocol for methylation timing; Quantitation. - [Read Replication Timing Using Transient Hemimethylation Protocol]

SDS-PAGE - http://www.ciwemb.edu/labs/koshland/Protocols/Protein/sdspage.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS-PAGE: gel electrophoresis of proteins. Very simple protocol for SDS-PAGE. Koshland Lab, Carnegie Institution of Washington, Howard Hughes Medical Institute. - [Read SDS-PAGE]

Standard Long PCR - http://pga.gs.washington.edu/protocols/pcr_protocol.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

Standard Long PCR. This is a standard PCR protocol used on all first pass (unoptimized) PCR amplifications. UW-FHCRC - [Read Standard Long PCR]

Standard Long PCR - http://pga.gs.washington.edu/protocols/pcr_protocol.html

Category: PCR Protocols: Standard PCR Protocol

Standard Long PCR. This is a standard PCR protocol used on all first pass (unoptimized) PCR amplifications. UW-FHCRC - [Read Standard Long PCR]

Steroid Radioimmunoassay Protocol - http://faculty.washington.edu/jwingfie/protocols.htm

Category: Immunology: Immunoassays

Protocol for steroid radioimmunoassay. Includes: SOLVENT DISTILLATION; PREPARATION OF PLASMA SAMPLES; EXTRACTION OF STEROIDS AND COLUMN PACKING; COLUMN CHROMATOGRAPHY; RADIOIMMUNOASSAY; SEPARATION OF BOUND AND FREE COUNTS; DIRECT ASSAYS; SHORT COLUMN CHROMATOGRAPHY. - [Read Steroid Radioimmunoassay Protocol]

Tandem Affinity Purification (TAP) Protocol - http://depts.washington.edu/~yeastrc/ms_tap1.htm

Category: Mass Spectrometry Protocols

Tandem Affinity Purification (TAP) Protocol. Modified from Rigaut et al. 1999. Univ.Washington Yeast Resource Center. - [Read Tandem Affinity Purification (TAP) Protocol]

Widefield Microscopy Nikon Upright with Operating Instructions - http://depts.washington.edu/keck/Widefield/equipment.htm

Category: Molecular Imaging

Widefield Microscopy Nikon Upright with Operating Instructions. Keck Imaging Center. - [Read Widefield Microscopy Nikon Upright with Operating Instructions]

Zeiss Meta Confocal Microscopy Imaging Instructions - http://depts.washington.edu/keck/Zeiss/Zeiss%20Instructions.html

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Zeiss Meta Confocal Microscopy Imaging Instructions. Operating Instructions with photos included. Keck Imaging Center. - [Read Zeiss Meta Confocal Microscopy Imaging Instructions]

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