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A Method for Micro-Scale Isolation and Purification of Gangliosides - http://www.glycotech.com/protocols/Proto1.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

Solvent partition protocol allows the isolation of gangliosides from small samples and from samples where ganglioside concentrations are low, especially relative to the concentration of potentially contaminating proteins and other large molecular weight species. Stephan Ladisch Director, Center for Cancer and Transplant Biology, Children's National Medical Center, Washington, D.C. - [Read A Method for Micro-Scale Isolation and Purification of Gangliosides]

AFLP: not only for fingerprinting, but for positional cloning - http://www-ciwdpb.stanford.edu/publications/methods/aflp.html

Category: PCR Protocols: AFLP PCR

Mannie Liscum and Paul Oeller. Department of Plant Biology. Carnegie Institution of Washington, Stanford. AFLP technology is used here to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been i - [Read AFLP: not only for fingerprinting, but for positional cloning]

Apoptosis Pathway Diagram Small - http://egp.gs.washington.edu/apop.html

Category: Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Information

Apoptosis Pathway Diagram Small - [Read Apoptosis Pathway Diagram Small]

Biogenesis and Function of Peroxisomes and Glycosomes - http://students.washington.edu/mdminer/pabio_551/kessler1.pdf

Category: Cell Organelle Protocols: Subcellular Fractionation Protocols: Peroxisome Isolation Protocols

Peroxisomes of higher eukaryotes, glycosomes of kinetoplastids, & glyoxysomes of plants are related microbody organelles that perform differing metabolic functions tailored to their cellular environments. The close evolutionary relationship of these organelles is most clearly evidenced by the conservation of proteins involved in matrix protein import and biogenesis. glycosome can be viewed as an offshoot of the peroxisomal lineage with additional metabolic functions, specifically glycolysi - [Read Biogenesis and Function of Peroxisomes and Glycosomes]

Centrifuge Safety - High Speed Hazards - http://www.ehs.wsu.edu/Factsheets/FAQCentrifuge.asp

Category: General Research Protocols and Methods: Centrifuge Use and Centrifugation

Centrifuge Safety - High Speed Hazards. Safe Centrifuge Use , Care and Maintenance, and maintaining a log book for centrifuges. EHS Washington state Univ. - [Read Centrifuge Safety - High Speed Hazards]

Chemokine Receptor Expression Assay by Flow Cytometry - http://ubik.microbiol.washington.edu/protocols/facs/chemokinereceptorfacs.htm

Category: Signalling Signal Transduction Protocols: Chemokine Protocols

Chemokine Receptor Expression Assay by Flow Cytometry. Mullins Lab. Reagents, Protocol and detailed procedure. - [Read Chemokine Receptor Expression Assay by Flow Cytometry]

Confocal Microscopy and Protocols - http://depts.washington.edu/keck/intro.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Confocal Microscopy and Protocols. Why use a confocal microscope? Fluorescence, Reflectance or Transmission? Which confocal microscope should you use? Confocal or 2-photon microscopy? Sample Preparation for confocal microscopy, Fixation, Immunolabeling, - [Read Confocal Microscopy and Protocols]

Deltavision Deconvolution Instructions - http://depts.washington.edu/keck/dv/start.html

Category: Molecular Imaging

Deltavision Deconvolution Instructions. Keck Imaging Center. - [Read Deltavision Deconvolution Instructions]

DNA Content using Hoechst 33342 in Unfixed Cells Protocol - http://depts.washington.edu/immunweb/cellanalysis/HoeDNA.html

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry

Protocol for DNA content using Hoechst 33342 in unfixed cells. - [Read DNA Content using Hoechst 33342 in Unfixed Cells Protocol]

DNA digestion protocol & hints PDF - http://www.crypto.wustl.edu/Protocols%20PDFs/RE%20digests%20(HL).pdf

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

DNA digestion protocol & hints. Procedure and tips for restriction digestion. Doering laboratory, Department Molecular Microbiology, Washington University Medical School. - [Read DNA digestion protocol & hints PDF]

FP-TDI Method for SNP Detection - http://las.perkinelmer.com/content/snps/fp-tdi.asp

Category: DNA Protocols: SNP Protocols

FP-TDI Method for SNP Detection. The FP-TDI protocol was originally reported by Drs. Chen, Levine, and Kwok at Washington University in 19991,2. FP-TDI stands for template directed dye terminator incorporation assay with detection by fluorescence polarization. It is a single base primer extension assay couple with homogeneous FP detection. Perkin Elmer - [Read FP-TDI Method for SNP Detection]

Gradient Purification of AT2 Inactivated HIV-1 Protocol - http://ubik.microbiol.washington.edu/protocols/bl3/at2.htm

Category: Viral Protocols

Protocol for gradient purification of AT2 inactivated HIV-1. - [Read Gradient Purification of AT2 Inactivated HIV-1 Protocol]

Hairpin-bisulfite PCR: Assessing epigenetic - http://staff.washington.edu/miner/hpbspcr/Laird%20et%20al%202004.pdf

Category: Epigenetics and Methylation Protocols: Bisulfite Treatment Methylation Assay Protocol

Hairpin-bisulfite PCR will be a powerful tool in studying other processes for which genetic or epigenetic information differs on the two complementary strands of DNA. Laird et al., 2003. PNAS - [Read Hairpin-bisulfite PCR: Assessing epigenetic]

HIV-1 p24 Antigen Capture ELISA Protocol - http://ubik.microbiol.washington.edu/protocols/bl3/p24elisa.htm

Category: ELISA Protocols

Protocol for HIV-1 p24 antigen capture ELISA. - [Read HIV-1 p24 Antigen Capture ELISA Protocol]

HIV-1 p24 Antigen Capture ELISA Protocol - http://ubik.microbiol.washington.edu/protocols/bl3/p24elisa.htm

Category: Viral Protocols

Protocol for HIV-1 p24 antigen capture ELISA. - [Read HIV-1 p24 Antigen Capture ELISA Protocol]

Image Processing Tools - http://depts.washington.edu/keck/leica/ip.html

Category: Molecular Imaging

Image Processing Tools. Keck Imaging Center - [Read Image Processing Tools]

Immunofluorescence / confocal microscopy - http://depts.washington.edu/keck/susan.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

B or T cells in suspension, adherent cells on chambered coverglass or chamberslides, cryostat sections of unfixed, OCT embedded tissue. Susan Anderson. - [Read Immunofluorescence / confocal microscopy]

Leica Confocal Instructions - http://depts.washington.edu/keck/leica/instructions.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Leica Confocal Instructions. Keck Imaging Center. - [Read Leica Confocal Instructions]

Leica SL Confocal Microscopy Imaging Instructions - http://depts.washington.edu/keck/LeicaSL/SL%20Instructions.htm

Category: Molecular Imaging: Confocal Microscopy Protocols and Information

Leica SL Confocal Microscopy Imaging Instructions. Keck Imaging Center. - [Read Leica SL Confocal Microscopy Imaging Instructions]

Live Cell Prep for Sorting Protocol - http://depts.washington.edu/immunweb/cellanalysis/Sortprp.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Cell Sorting Protocols

Protocol for live cell prep for sorting. - [Read Live Cell Prep for Sorting Protocol]

Modified Topo T/A cloning protocol - http://mullinslab.microbiol.washington.edu/protocols/other/TopoTACloning.doc

Category: DNA Protocols: TOPO TA Cloning

Modified Topo T/A cloning protocol. Mullins Lab. Univ. Washington. - [Read Modified Topo T/A cloning protocol]