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A Triple Color FISH Technique for Mouse Chromosome Identification - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html

Category: BACs Bacterial Artificial Chromosome Protocols

Describe a set of BACs that map close to the centromeric and distal chromosomal ends for each mouse chromosome. - [Read A Triple Color FISH Technique for Mouse Chromosome Identification]

Alexa Dyes, Cy Dyes and Other Fluors: Succinimidyl Esters - http://info.med.yale.edu/genetics/ward/tavi/FISHdyes.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Alexa Dyes, Cy Dyes and other Fluors: succinimidyl esters. - [Read Alexa Dyes, Cy Dyes and Other Fluors: Succinimidyl Esters]

Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative - http://info.med.yale.edu/genetics/ward/tavi/CCKmethod.html

Category: Cytogenetics Protocols

Protocol for color changing karytoping (CCK): an M-FISH/SKY alternative. Includes: Slide preparation and hybridization; Probes, dyes and antibodies; Probe detection and image capture; Image processing. - [Read Color Changing Karyotyping (CCK) Protocol: An M-FISH/SKY Alternative]

Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/TMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for cryptic translocation identification in human and mouse using several telomeric multiplex FISH (TM-FISH) strategies. - [Read Cryptic Translocation Identification in Human and Mouse Using Several Telomeric Multiplex FISH]

Custom Fluorescent Nucleotide Synthesis/Nucleic Acid Labeling Protocol - http://info.med.yale.edu/genetics/ward/tavi/n_coupling.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for custom fluorescent nucleotide synthesis/nucleic acid labeling. - [Read Custom Fluorescent Nucleotide Synthesis/Nucleic Acid Labeling Protocol]

FISH Guide and Troubleshooting - http://info.med.yale.edu/genetics/ward/tavi/FISHguide.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

FISH Guide and Troubleshooting. Includes: Cytogenetic techniques; Slide preparation; When do chromosomes spread; Cell suspensions; G-banding; Cell fixatives; Slide preparation factors. - [Read FISH Guide and Troubleshooting]

G-Banding Observations - http://info.med.yale.edu/genetics/ward/tavi/slide08.html

Category: Cytogenetics Protocols

G-Banding observations. Includes: Slide storage; Fixative composition and trypsin treatment in G-banding; Banding of chemically aged slides. - [Read G-Banding Observations]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: PCR Protocols: PCR Optimization

A Great PCR and Multiplex Guide. Topics for PCR optimization. Primer conditions, PCR temperatures, etc. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR and Multiplex Guide - http://info.med.yale.edu/genetics/ward/tavi/Guide.html

Category: PCR Protocols: Standard PCR Protocol

PCR and Multiplex Guide. Amazing Guide to primer amount, MgCl2 amount, annealing temperature, and more. With picture examples. Octavian Henegariu. - [Read PCR and Multiplex Guide]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: PCR Buffer Protocols

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

PCR Buffers Protocol - http://info.med.yale.edu/genetics/ward/tavi/p06.html

Category: PCR Protocols: Standard PCR Protocol

Protocol for PCR buffers. - [Read PCR Buffers Protocol]

PCR Program Design - http://info.med.yale.edu/genetics/ward/tavi/p08.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

The requirement of an optimal PCR reaction is to amplify a specific locus without any unspecific by-products. Therefore, annealing needs to take place at a sufficiently high temperature to allow only the perfect DNA-DNA matches to occur in the reaction. P - [Read PCR Program Design]

PCR Program Design - http://info.med.yale.edu/genetics/ward/tavi/p08.html

Category: PCR Protocols: PCR Optimization

Slide Preparation for Cytogenetics Protocol - http://info.med.yale.edu/genetics/ward/tavi/Cytom-slideprep.html

Category: Cytogenetics Protocols

Protocol for slide preparation for cytogenetics. Includes: Improved cell recovery by microcentrifugation; Slide storage; "Controlled" chromosome spreading; Dropping cells from a height does not improve spreading; GTG-banding. - [Read Slide Preparation for Cytogenetics Protocol]

Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH - http://info.med.yale.edu/genetics/ward/tavi/CMFISH.html

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for small marker chromosome identification in metaphase and interphase using centromeric multiplex FISH (CM-FISH). This approach allows the identification of marker chromosomes and other aneuploides on one single cytogenetic slide, in less than two hours. - [Read Small Marker Chromosome Identification in Metaphase and Interphase using Centromeric Multiplex FISH]

The Virtual Mouse Necropsy - http://www.geocities.com/virtualbiology/

Category: Animal Research Techniques

Perform a Mouse Necropsy on the Web. Dr. J.M.Ward - [Read The Virtual Mouse Necropsy]

Triple Color FISH Technique for Mouse Chromosome Identification Protocol - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html

Category: Mouse Protocols: Mouse Genetics Protocols

Protocol for triple color FISH technique for mouse chromosome identification. - [Read Triple Color FISH Technique for Mouse Chromosome Identification Protocol]

Troubleshooting for PCR and multiplex PCR - http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Troubleshooting for PCR and multiplex PCR - http://info.med.yale.edu/genetics/ward/tavi/Trblesht.html

Category: PCR Protocols: PCR Troubleshooting

Very Good Troubleshooting for PCR and multiplex PCR Guide. Octavian Henegariu. - [Read Troubleshooting for PCR and multiplex PCR]

Articles (1-2 of 2)

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Picking Transfected ES Cell Clones Protocol

This protocol a protocol on how to generate transfected embryonic stem (ES) cell clones. The previous protocol in this series is the Protocol for Electroporation of ES cells. The next protocol in the series is the Protocol on Disaggregation, Expansion, and Freezing of Transfected ES Clones.

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