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Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography - http://www.glycotech.com/protocols/Proto6.html

Category: Molecular Biology Protocols: Carbohydrate Protocols

High performance liquid affinity chromatography (HPLAC) is a useful procedure to investigate he interactions between carbohydrate binding protein and their ligands. Technical requirements are similar to conventional HPLC. HPLAC can screen and separate natural ligands from complex biological mixtures. WeiTong Wang~GlycoTech Corporation, Rockville, Maryland - [Read Analysis of Oligosaccharide Ligands by High Performance Liquid Affinity Chromatography]

Enzyme Purification by Acetone Precipitation - http://www.glue.umd.edu/~nsw/ench485/lab6b.htm

Category: Protein Protocols: Protein Precipitation Procols

Acetone Precipitation Protocol and Method. Nam Sun Wang. Univ. Maryland. - [Read Enzyme Purification by Acetone Precipitation]

Enzyme Purification by Salt Ammonium Sulfate Precipitation - http://www.ece.umd.edu/~nsw/ench485/lab6a.htm

Category: Protein Protocols: Protein Precipitation Procols

ENZYME PURIFICATION BY SALT (AMMONIUM SULFATE) PRECIPITATION. Prepared by Nam Sun Wang, Department of Chemical & Biomolecular Engineering. University of Maryland. - [Read Enzyme Purification by Salt Ammonium Sulfate Precipitation]

Fluorescent Staining of Cells - http://ylwang.umassmed.edu/protocol/cfs/stain.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

Fluorescent Staining of Cells protocol. Yu-li Wang Lab. Fluorescent Phalloidin Staining, Immunofluorescence Staining and procedures for staining. - [Read Fluorescent Staining of Cells]

Lowry Protein Assay Protocol - http://ylwang.umassmed.edu/protocol/pa/lowry.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay Protocol. Yu-li Wang Lab, Univ. Massachusetts Medical School. Detailed protocol including materials. - [Read Lowry Protein Assay Protocol]

PC12 Cell Culture and Fusion - http://ylwang.umassmed.edu/protocol/cc/pc12.htm

Category: Cell Biology and Cell Culture: Cell Line Culture Conditions

PC12 Cell Culture and Fusion Methods. Yu-li Wang - [Read PC12 Cell Culture and Fusion]

Preparation of Dialysis Tubing Protocol - http://ylwang.umassmed.edu/protocol/pp/dialysis.htm

Category: Protein Protocols: Protein Dialysis Protocols

Preparation of Dialysis Tubing. Yu-Li Wang Lab - [Read Preparation of Dialysis Tubing Protocol]

Protocol for Real-Time RT-PCR Using SYBR Green - http://pga.mgh.harvard.edu/primerbank/PCR_protocol.html

Category: Real Time PCR

A detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Wang et al 2003. - [Read Protocol for Real-Time RT-PCR Using SYBR Green]

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN - http://ylwang.umassmed.edu/protocol/cfs/dbp.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN. Indirect and direct methods. Yu li Wang labs. - [Read STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEIN]

Vital Staining of Chromosomes with Hoechst 33258 - http://ylwang.umassmed.edu/protocol/cfs/hoechst.htm

Category: Molecular Imaging: Immunofluorescence Microscopy

Vital Staining of Chromosomes with Hoechst 33258. Yu Li Wang labs. - [Read Vital Staining of Chromosomes with Hoechst 33258]