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Transformation of E. Coli by Electroporation Protocol. - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3933

Category: Transfection Protocols: Electroporation Sonoporation Protocols

Electrocompetent bacteria are prepared by growing cultures tomid-log phase, washing the bacteria extensively at low temperature,and then resuspending them in a solution of low ionic strengthcontaining glycerol. DNA is introduced during exposure of thebacteria to a short high-voltage electrical discharge.

- [Read Transformation of E. Coli by Electroporation Protocol.]

Whole-Cell Voltage Clamp of Isolated Heart Cells - http://www.southalabama.edu/ishr/help/wholecellclamp/

Category: Neuroscience Protocols: Patch Clamping Protocols

This article briefly summarizes the use of this variant of the patch-clamp technique in isolated cardiac myocytes with an emphasis on practical suggestions. - [Read Whole-Cell Voltage Clamp of Isolated Heart Cells]

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Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.