User Panel

My Panel

Bookmark Science Articles

vitro Protocols

Search results for: vitro

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

In Vitro MAP Kinase Assay - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/In%20Vitro%20MAP%20kinase%20assay%20%209.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describes an in vitro assay to detect MAP kinase activity using PC12 cells. - [Read In Vitro MAP Kinase Assay]

In Vitro Micromass Teratogen Assay - http://embryo.ib.amwaw.edu.pl/invittox/prot/114.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cell Specific Cytotoxicity Assays Protocols

The effect of a test compound, in the presence and absence of S-9 mix, on the differentiation and growth of rat limb bud and CNS cells in vitro indicates whether it is potentially a teratogen in vivo. - [Read In Vitro Micromass Teratogen Assay]

In Vitro Model For Studies Of Prostagland H Synthase (PHS)- Mediated Genotoxicity Of Xenobiotics - http://embryo.ib.amwaw.edu.pl/invittox/prot/61.htm

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

This protocol describes the use of SEMV cells (a cell line derived from ram seminal vesicles) in studies into prostaglandin H synthase-mediated metabolism of xenobiotics in intact cells. - [Read In Vitro Model For Studies Of Prostagland H Synthase (PHS)- Mediated Genotoxicity Of Xenobiotics]

In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3813

Category: Cloning Protocols: Mutagenesis Protocols

Protocol for in vitro mutagenesis using double-stranded DNA templates. Two oligonucleotides are used to prime DNA synthesis catalyzed by a high-fidelity thermostable polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and occupy the same starting and ending positions on opposite strands of the plasmid DNA. - [Read In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI]

In Vitro Prediction of the Maximum Tolerated Dose Protocol - http://embryo.ib.amwaw.edu.pl/invittox/prot/66.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Liver Toxicity Assays Protocols

The results of cytotoxicity tests in primary cultures of rat hepatocytes and in MDBK and McCoy cells can be used to predict the in vivo 4-wk maximum tolerated dose in rats and dogs. A correlation between in vitro cytotoxicity, as measured in this system, and LD50 values in rats and mice has also been established. - [Read In Vitro Prediction of the Maximum Tolerated Dose Protocol]

In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters - http://www.nottingham.ac.uk/~mbzspd/methods/In_vitro_RNA_synthes.html

Category: RNA Protocols: In Vitro Transcription T7 SP6 T3 Protocols

RNA, in vitro synthesis using T3 T7 promoters. Dr. Dawson Univ. Nottingham. - [Read In vitro RNA synthesis from plasmid-borne sequences under the control of T phage promoters]

In Vitro Splicing Reactions in Drosophila KC or HeLa Nuclear Extracts - http://www.bio.com/protocolstools/protocol.jhtml%3Bjsessionid%3D34E3CBZRBQMFZR3FQLMCFEWHUWBNSIV0?id=p519

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro splicing reactions in Drosophila KC or HeLa nuclear extracts. Protocol includes: Procedure, Solutions, BioReagents and Chemicals and protocol hints. - [Read In Vitro Splicing Reactions in Drosophila KC or HeLa Nuclear Extracts]

In Vitro System to Analyze Light Signal Transduction in Neurospora crassa - http://www.fgsc.net/neurosporaprotocols/How%20to%20analyze%20light%20signal%20transduction%20in%20vitro.pdf

Category: Fungi Protocols: Neurospora Protocols

Procedure for an in vitro system to analyze light signal transduction in Neurospora crassa - [Read In Vitro System to Analyze Light Signal Transduction in Neurospora crassa]

In Vitro T Cell Activation - http://www.ebioscience.com/ebioscience/appls/AC145.htm

Category: Signalling Signal Transduction Protocols

Assess T cell activation protocol - measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. eBioscience - [Read In Vitro T Cell Activation]

In Vitro Transcription and Translation Using the Coupled Reticulocyte Lysate System - http://www.bio.com/protocolstools/protocol.jhtml?id=p594

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription and translation using the coupled reticulocyte lysate system. This protocol is designed to test random samples on a protein gel. Scale up the reactions accordingly. Protocol includes: Procedure, Solutions, BioReagents and Chemicals and protocol hints. - [Read In Vitro Transcription and Translation Using the Coupled Reticulocyte Lysate System]

In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells - http://www.bio.com/protocolstools/protocol.jhtml?id=p1971

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

With this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are used. Includes information on: Permeabilization of Cells; In vitro Transcription Reaction (Run-off); Isolation of RNA; Preparation of Slot Blot Membrane for Hybridization; Hybridization of Nitrocellulose Membrane; TCA Precipitation to Determine Incorporation of [32P] GTP into Nucleic Acid - [Read In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells]

in vitro Transcription Protocol - http://penguin.uchc.edu/~intron/Protocols/in%20vitro%20transcription.html

Category: RNA Protocols: In Vitro Transcription T7 SP6 T3 Protocols

Short and simple In Vitro Transcription Protocol. Brent Graveley, University of Connecticut Health Center. - [Read in vitro Transcription Protocol]

In Vitro Transcription Protocol - http://www.cvmbs.colostate.edu/mip/wiluszlab/protocols/invitrotrans.htm

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription. Includes information on: Hot transcription reactions, notes and troubleshooting. - [Read In Vitro Transcription Protocol]

In Vitro Transcription with SP6 Polymerase Protocol - http://www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=92

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription with SP6 polymerase protocol. - [Read In Vitro Transcription with SP6 Polymerase Protocol]

In Vitro Transcription With Yeast Nuclear Extract - http://www.fhcrc.org/science/labs/hahn/methods/biochem_meth/polii_trans.html

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol for in vitro transcription with yeast nuclear extract. Includes recipes for: 5x Acetate transcription buffer; Creatine phospho kinase; Phospho creatine; Stop mix; HA + 0.1 M potassium acetate; 5x glutamate transcription buffer (5 ml). Includes also protocol for Primer Extension Assay. - [Read In Vitro Transcription With Yeast Nuclear Extract]

In vitro Translation Assays for CFTR - http://pen2.igc.gulbenkian.pt/cftr/vr/c/ostedgaard_in_vitro_translation_assays_for_cftr.pdf

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol outlines the general procedure and requirements for in vitro translation of CFTR and outlines some assays using in vitro translated product. Core glycosylation of CFTR occurs in the ER. An assay for this processing step requires the addition of microsomal membranes to the basic in vitro translation mixture. This protocol takes this into account. - [Read In vitro Translation Assays for CFTR]

In Vivo Cloning with Mu Protocol - http://rothlab.ucdavis.edu/protocols/in-vitro-mu-cloning.html

Category: Cloning Protocols: Vector Protocols

Protocol for in vivo cloning with mu. - [Read In Vivo Cloning with Mu Protocol]

In-Vitro Kinase Assay for Jnk-1/IRS Proteins - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/In_vitro%20kinase%20assay%20for%20JNK-1%20IRS%20proteins.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describing an in vitro assay on the detection of Kinase activity for Jnk-1/IRS proteins. - [Read In-Vitro Kinase Assay for Jnk-1/IRS Proteins]

Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6634D5A5D8B91EC631DE5426302733&objectid=6674D9B6A53E5856FBF23C4E94630203

Category: Immunology: T Cell Protocols

Describes methods for labeling high or low numbers of lymphocytes with CSFE. Protocols are provided to use CSFE-labeled cells in cell transfer studies or as cells to be cultured in vitro. Detailed guidelines for positioning of CSFE-labeled lymphocytes in lymphoid organs or other tissues are included for those wishing to use this approach to study lymphocyte migration. - [Read Intracellular Fluorescent Dye CFSE to Monitor Lymphocyte Migration and Proliferation]

Kinase Assay Using In Vitro Translated Xenopus Mos Kinase - http://www.bio.com/protocolstools/protocol.jhtml?id=p912

Category: Signalling Signal Transduction Protocols

Protocol describes how tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay. Protocol includes: Translation of Xenopus Mos Kinase; Antibody to Antigen Binding; Protein A Sepharose to Antibody Binding; Kinase Reactions on Immunoprecipitated Material; Polyacrylamide Gel Analysis of Immunoprecipitates. Includes protocol hints. - [Read Kinase Assay Using In Vitro Translated Xenopus Mos Kinase]

Laser Capture Microdissection of Living in vitro Cells PDF - http://www.arcturusbiosciences.com/research_portal/images/Application%20Notes/live_cell_protocol_4.pdf

Category: Histology Protocols: Laser Capture and Microdissection

Laser Capture Microdissection of Living in vitro Cells. This PDF describes a precise, rapid and convenient Laser Capture Microdissection (LCM) method for the positive selection of living adherent cells and the successful subsequent re-cultivation of homogenous sub-populations. Arcturus. - [Read Laser Capture Microdissection of Living in vitro Cells PDF]

Media for Culture of Mammalian Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E662B6F09C1BB8FBC47FC2A7E61A8A3&objectid=66739B890A55AC13354418E67F28E693

Category: Cell Biology and Cell Culture: Cell Culture Growth Media: Mammalian Cell Culture Growth Media Protocols

The culture medium is an essential component of the in vitro environment and must be selected or designed with care. This protocol provides guidelines for design of serum-containing and serum-free media, selective and specialty media, and media for growth under special conditions such as soft-agar growth. - [Read Media for Culture of Mammalian Cells Protocol]

Microarray Protocols - http://www.erasmusmc.nl/interne_oncologie/res/endo/Protocol%20for%20RNA%20amplification%2C%20labelling%20and%20hybridization%20to%20%20microarray.pdf

Category: Microarray Protocols: Microarray Analysis Protocols

Protocols for microarrays. Includes: RNA amplification by in vitro transcription; cDNA labeling; Hybridization of cDNA microarrays. - [Read Microarray Protocols]

Molecular and Genetic Toxicology Studies for In Vitro Cytogenetics with CHO or human lymphocytes - http://www.sri.com/biosciences/bdd/toxic/protocol.html#7

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies for in vitro cytogenetics with CHO or human lymphocytes. Includes: G401 Chromosomal Aberrations in Chinese Hamster Ovary (CHO) Cells, G401-2, G401-R, G401-R2, G401-R2H, G402 Chromosomal Aberrations in Human Lymphocytes, G402-2, G402-R, G402-R2, G402-R2H, G403 Sister Chromatid Exchanges in Chinese Hamster Ovary (CHO) Cells. - [Read Molecular and Genetic Toxicology Studies for In Vitro Cytogenetics with CHO or human lymphocytes]

Molecular and Genetic Toxicology Studies on Gene Mutation in Mammalian Cells In Vitro - http://www.sri.com/biosciences/bdd/toxic/protocol.html#5

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols

Molecular and genetic toxicology studies on gene mutation in mammalian cells In vitro. Includes: G201 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Assay; G201-R; G202 Mouse Lymphoma L5178Y/tk+/Cell Gene Mutation Screening Assay; G203 Chinese Hamster Ovary or V79 Cell Mutation Assay at the hprt Locus; G203R. - [Read Molecular and Genetic Toxicology Studies on Gene Mutation in Mammalian Cells In Vitro]

Articles (1-1 of 1)

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Items 26-50 out of 90 displayed.

First Previous 1 [2] 3 4 Next Last

Total records: 90