viruses Protocols

Search results for: viruses

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

M5 Velocity (rate zonal) gradients for purification and assembly analysis of viruses. - http://www.axis-shield.com/densityhome/optiprep/M05.pdf

Category: Cell Biology and Cell Culture

Purification protocols of the viruses: HIV-1, Lassa virus, oncornavirus and other retroviruses. Protocol uses an iodixanol gradient in a sedimentation velocity mode to purifyHIV-1 virions without affecting the infectivity of the virus. In rate-zonal iodixanol gradients the HIV-1 was effectively separated both from Vif and from the microvesicles. - [Read M5 Velocity (rate zonal) gradients for purification and assembly analysis of viruses.]

Preferential Delivery of the Sleeping Beauty transposon System to Livers of Mice by Hydrodynamic i - http://www.natureprotocols.com/2007/12/06/preferential_delivery_of_the_s.php

Category: Developmental Biology: Utilizing Model Organisms Protocols

Nonviral, DNA-mediated gene transfer is an alternative to viral delivery systems for expressing new genes in cells and tissues. The Sleeping Beauty (SB) transposon system combines the advantages of viruses and naked DNA molecules for gene therapy purposes; however, efficacious delivery of DNA molecules to animal tissues can still be problematic. Here we describe the hydrodynamic delivery procedure for the SB transposon system that allows efficient delivery to the liver in the mouse. - [Read Preferential Delivery of the Sleeping Beauty transposon System to Livers of Mice by Hydrodynamic i]

Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues. - http://www.axis-shield.com/densityhome/optiprep/M17.pdf

Category: Cell Biology and Cell Culture

Summary of the techniques used for the purification of viruses using pre-formed iodixanol gradients. Viruses purified include: Semliki Forest, Retrovirus (from human melanoma cells), Rabies, Polyoma, Papillomavirus, Lassa, Influenza, Bovine viral diarrhea. - [Read Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues.]

Titering of Bacterial Viruses Protocol - http://biology.clc.uc.edu/fankhauser/Labs/Microbiology/Phage/Phage_Titer.htm

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

When an individual bacterial virus grows in a bacterial host suspended in a top agar lawn, its progeny infect and lyse the surrounding host cells. This causes the appearance of a "hole" or plaque in the otherwise homogeneous bacterial lawn. Since each plaque represents a single virus, the number of viruses in the aliquot added to the plate is equal to the number of plaques which appear. - [Read Titering of Bacterial Viruses Protocol]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]