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A Strategy for the Rapid Identification of - http://www.people.virginia.edu/~wrp/papers/mcp_wrp02b.pdf

Category: Protein Protocols: Protein Phosphorylation Protocols

A Strategy for the Rapid Identification of Phosphorylation Sites in the Phosphoproteome. MacDonald et al., Molecular & Cellular Proteomics. 2002. - [Read A Strategy for the Rapid Identification of]

Antigen Retrieval for immunohistochemistry - http://herpesvirus.tripod.com/research/protoc.htm#Antigen%20Retrieval%20for

Category: Immunology: Antigen Retrieval

Antigen Retrieval for immunohistochemistry. Microwave method, Autoclave method. slides and staining. Janos Luka. Eastern Virginia. - [Read Antigen Retrieval for immunohistochemistry]

cDNA Library Screening Protocol - ZAPII - http://www.people.virginia.edu/~gwl6s/protocols/cdna.html

Category: RNA Protocols: cDNA Libraries Library

cDNA Library Screening Protocol - ZAPII Detailed Protocol. XLI-Blue MRF' and cDNA phage library screening. Dr. Laurie. University of Virginia Health Science Center - [Read cDNA Library Screening Protocol - ZAPII]

Cell Adhesion Protocol - http://people.virginia.edu/~gwl6s/protocols/adhesion.html

Category: Cell Biology and Cell Culture

Protocol for cell adhesion. - [Read Cell Adhesion Protocol]

General EMSA Protocol - http://faculty.virginia.edu/mirmira/Protocols/emsa.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

There are multiple variations to this protocol, but they find that this one works well in all cases tested. Mirmira Lab. - [Read General EMSA Protocol]

Immunostaining of Cells Adherent to Coverslips - http://www.people.virginia.edu/~gwl6s/protocols/cellstain.html

Category: Immunology: Immunocytochemistry Protocols

Laurie Lab, Univ. Virginia. - [Read Immunostaining of Cells Adherent to Coverslips]

Laboratory Survival Manual - http://keats.admin.virginia.edu/lsm/home.html

Category: General Research Protocols and Methods

Laboratory Survival Manual. Lab Safety, First AID, Chemicals and Equipment. Vaughn C. Kowahl - [Read Laboratory Survival Manual]

Mass spectrometry analysis and identification of proteins - http://www.healthsystem.virginia.edu/internet/biomolec/mass_spectrometry.cfm

Category: Mass Spectrometry Protocols

Protein sequencing by mass spectrometry. Mass analysis of peptides and proteins. Identification of modifications in proteins. Good information.Univ. Virginia. Dr. Nicholas Sherman - [Read Mass spectrometry analysis and identification of proteins]

Methanol-Chloroform Precipitation of Proteins - http://www.healthsystem.virginia.edu/internet/biomolec/methanolchloroform.cfm

Category: Mass Spectrometry Protocols

Methanol-Chloroform Precipitation of Proteins. A procedure for precipitating proteins from solution, including detergent solutions, is from Wessel and FlÃ¼gge (1984). Univ. Virginia. - [Read Methanol-Chloroform Precipitation of Proteins]

Mirmira Lab ChIP Protocol - http://faculty.virginia.edu/mirmira/resources_files/Protocols/MirmiraLabChIPprotocol.htm

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Mirmira Lab ChIP Protocol. 50-step protocol forformaldehyde crosslinking to ChIP and also provides recipes for all buffer reagents. - [Read Mirmira Lab ChIP Protocol]

Preparation of Broth and Plates - http://www.people.virginia.edu/~gwl6s/protocols/recipes.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

Terrific Broth, LB plates, NZY Plates, LB Broth. Laurie Lab, Univ. Virginia Health Science Center. - [Read Preparation of Broth and Plates]

Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots - http://faculty.virginia.edu/mirmira/resources_files/Protocols/Nuclear%20Extracts.htm

Category: Protein Protocols: Nuclear Extraction Protocols

Nuclear Extract protocol is optimized for about 10^7 cells, can use for gel shift assays and western blotting. Mirmira Lab, Univ. Virginia - [Read Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots]

Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis - http://www.healthsystem.virginia.edu/internet/biomolec/2dgelsampleprep.cfm

Category: Proteomic Protocols

Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis. Univ. Virginia. - [Read Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis]

Radiolabeling of Probes for Electrophoretic Mobility Shift Assay - http://faculty.virginia.edu/mirmira/resources_files/Protocols/Radiolabeling.htm

Category: DNA Protocols: EMSA DNA-Protein Protocols

Creation of Probe for EMSA using Oligonucleotide annealing, p-32, and T4 Polynucleotide Kinase. - [Read Radiolabeling of Probes for Electrophoretic Mobility Shift Assay]

Radiolabeling of Probes for Electrophoretic Mobility Shift Assays Protocol - http://faculty.virginia.edu/mirmira/resources_files/Protocols/Radiolabeling.htm

Category: DNA Protein Interactions Protocols: Electrophoretic Mobility Shift Assays EMSA Protocols

Protocol for radiolabeling of probes for electrophoretic mobility shift assays. - [Read Radiolabeling of Probes for Electrophoretic Mobility Shift Assays Protocol]

Silver Staining of Gels for Mass Spectrometry - http://www.healthsystem.virginia.edu/internet/biomolec/Silverstains.cfm

Category: Mass Spectrometry Protocols

Silver Staining of Gels for Mass Spectrometry. Desiderio procedure: R.R. Becklin. Univ. Virginia. - [Read Silver Staining of Gels for Mass Spectrometry]

Tissue sectioning and in situ hybridization as prepared by Simon Dunn PDF - http://oregonstate.edu/~weisv/Protocols/Cellular/Tissue%20sectioning%20and%20in%20situ%20hybridization%20Protocol.pdf

Category: Histology Protocols: Tissue Sample Sectioning

Tissue sectioning and in situ hybridization as prepared by Simon Dunn PDF. Includes Reagents and Solution recipes. Weis Lab, Virginia. - [Read Tissue sectioning and in situ hybridization as prepared by Simon Dunn PDF]

Tryptic digestion directly in polyacrylamide gel slices - http://www.healthsystem.virginia.edu/internet/biomolec/ingeldigest.cfm

Category: Mass Spectrometry Protocols

Tryptic digestion directly in polyacrylamide gel slices. A. Shevchenko et al. Univ. Virginia. - [Read Tryptic digestion directly in polyacrylamide gel slices]

Yeast Two-Hybrid Screen with Library and Bait - http://faculty.virginia.edu/mirmira/resources_files/Protocols/YEAST%20TWO.htm

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

yeast transformed with bait vector. yeast culture. selection plates. Mirmira, Univ. Virginia. - [Read Yeast Two-Hybrid Screen with Library and Bait]