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A Simplified System for Rapid Generation of Recombinant Adenoviruses - http://www.coloncancer.org/adeasy/protocol.htm

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

A simplified system for rapid generation of recombinant adenoviruses. Includes: Generation of Recombinants in Bacterial Cells; Viral Production in 293 or 911 Cells; Preparation of High Titer Viral Stocks. - [Read A Simplified System for Rapid Generation of Recombinant Adenoviruses]

Antibody Purification using Ammonium Sulphate Precipitation - http://www.icp.ucl.ac.be/mexp/vipg_pur.html

Category: Protein Protocols: Antibody Purification Protocols

Antibody Purification using Ammonium Sulphate Precipitation. Viral Immunity and Pathogenesis Group. - [Read Antibody Purification using Ammonium Sulphate Precipitation]

Bacterial Cell Maintenance Protocol - http://www.genome.ou.edu/protocol_book/protocol_partI.html#I.G

Category: Bacterial Cell Culture Protocols: Bacterial Stocks Protocols

4 strains of E. coli are used in these studies: JM101 for M13 infection and isolation, XL1BMRF'for M13 or pUC-based DNA transformation, and ED8767 for cosmid DNA transformation. To maintain their respective F' episomes necessary for M13 viral infection, JM101 is streaked onto a M9 minimal media plate and XL1BMRF' is streaked onto an LB plate containing tetracycline. ED8767 is streaked onto an LB plate. These plates are incubated at 37degC overnight. For each strain, 3 ml. of appropriate liquid.. - [Read Bacterial Cell Maintenance Protocol]

Cytokine Bioassays Protocols - http://www.ebioscience.com/ebioscience/appls/BAC.htm?

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Cytokine-Induced Proliferation of Indicator Cell Lines, TNF-Î±-Induced Killing of L929 Cell Line, IFN-Î³ Protection from Viral Infection of Cell Lines. eBiosciences. - [Read Cytokine Bioassays Protocols]

Cytokine Neutralization, In Vitro Using Antibodies - http://www.ebioscience.com/ebioscience/appls/NU.htm

Category: Signalling Signal Transduction Protocols: Cytokine Protocols

Antibody Neutralization of TNF-Î±-Induced Killing of L929 Cell Line, IFN-Î³-Protection from Viral Infection of L929 and A549 Cell Lines, TNF-Î±-Induced Killing of L929 Cell Line. eBiosciences. - [Read Cytokine Neutralization, In Vitro Using Antibodies]

ELISA for detection of platelet-associated Ig (PAIg) - http://www.icp.ucl.ac.be/mexp/vipg_plelisa.html

Category: ELISA Protocols

Qualitative ELISA (Enzyme-Linked Immunosorbent Assay) used for screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg). Andrei Musaji Viral immunity and pathogenesis group, Universite Catholique de Louvain. - [Read ELISA for detection of platelet-associated Ig (PAIg)]

Erythrophagocytosis Assay - http://www.icp.ucl.ac.be/mexp/vipg_eph.html

Category: Immunology: Macrophage Monocyte Protocols

Erythrophagocytosis Assay. The erythrophagocytosis assay is performed to compare the phagocytic rate with and without anti-eythrocyte antibody in either macrophages from control animals or virus-infected counterparts. Andrei Musaji Viral Immunity and Pathogenesis Group - [Read Erythrophagocytosis Assay]

Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Formamide Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3973

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

Formamide can be used instead of proteinase K to dissociate bacteriophage {lambda} coat proteins from the viral {lambda}. The procedure is rapid and works best with large-scale preparations of bacteriophage {lambda}. - [Read Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Formamide Protocol]

Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Proteinase K and SDS Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3972

Category: Viral Manipulation Protocols: Phage Lamda Manipulation Protocols

DNA is best isolated from bacteriophage lambda particles by digesting the viral coat proteins with a powerful protease such as proteinase K, followed by extraction with phenol:chloroform. This procedure is easily adapted for use with smaller-scale preparations of bacteriophage lambda. - [Read Extraction of Bacteriophage lambda DNA from Large-scale Cultures Using Proteinase K and SDS Protocol]

Growing Bacteriophage M13 in Liquid Culture Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3994

Category: Viral Culture and Isolation Protocols: Viral Culture Protocols

Most manipulations with M13, including preparations of viral stocks and isolation of single- and double-stranded DNAs, begin with small-scale liquid cultures that are infected with an M13 plaque, picked from an agar plate. - [Read Growing Bacteriophage M13 in Liquid Culture Protocol]

Growth of Primary Cell Culture and Viral Vector Handling Protocols - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Growth of Primary Cell Culture and Viral Vector Handling Protocols]

Isolation and Growth of Primary Cell Cultures from Mouse Protocol - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Category: Mouse Protocols: Mouse Cell Culture Protocols

The procedures involve the isolation and growth of primary cell cultures from rodent and human tissue as well as the use of viral vectors for the introduction and expression of mammalian genes in cells in culture and in live rodents. - [Read Isolation and Growth of Primary Cell Cultures from Mouse Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes Protocol - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for the isolation of genomic and viral DNA from lymphocytes. Includes: Viral DNA in cytosolic fraction and Viral DNA in nuclear fraction integrated. - [Read Isolation of Genomic and Viral DNA from Lymphocytes Protocol]

Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip - http://www1.qiagen.com/literature/protocols/pdf/QG03.pdf

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for the isolation of genomic and viral DNA from lymphocytes using the QIAGEN® Genomic-tip. - [Read Isolation of Genomic and Viral DNA from Lymphocytes using the QIAGEN® Genomic-tip]

PCR IN SITU: NEW TECHNOLOGIES WITH SINGLE CELL RESOLUTION - http://wheat.pw.usda.gov/~lazo/methods/minn/chap-shoot2.fm.html

Category: PCR Protocols: In-situ PCR Protocols

PCR IN SITU: NEW TECHNOLOGIES WITH SINGLE CELL RESOLUTION FOR THE DETECTION AND INVESTIGATION OF VIRAL LATENCY AND PERSISTENCE. Staskus 1998 U.Minn. - [Read PCR IN SITU: NEW TECHNOLOGIES WITH SINGLE CELL RESOLUTION]

Preferential Delivery of the Sleeping Beauty transposon System to Livers of Mice by Hydrodynamic i - http://www.natureprotocols.com/2007/12/06/preferential_delivery_of_the_s.php

Category: Developmental Biology: Utilizing Model Organisms Protocols

Nonviral, DNA-mediated gene transfer is an alternative to viral delivery systems for expressing new genes in cells and tissues. The Sleeping Beauty (SB) transposon system combines the advantages of viruses and naked DNA molecules for gene therapy purposes; however, efficacious delivery of DNA molecules to animal tissues can still be problematic. Here we describe the hydrodynamic delivery procedure for the SB transposon system that allows efficient delivery to the liver in the mouse. - [Read Preferential Delivery of the Sleeping Beauty transposon System to Livers of Mice by Hydrodynamic i]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Antibodies for Lineage Depletion; Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells for Culture, Flow Cytometry, or Transplantaio]

Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/Hematopoietic.pdf

Category: Immunology

Protocol for purification of Lin-, c-kit+, Sca-1+ bone marrow cells for Culture, Flow Cytometry, or Transplantaion. Includes: Harvest Marrow; Lineage Depletion; Cell Sorting; Viral Transduction; Transplant; CFU assays; GFP and cell surface marker immunostaining. - [Read Purification of Lin-, c-kit+, Sca-1+ Bone Marrow Cells Protocol]

Stain for Viral Inclusions Phloxine Tartrazine Protocol - http://www.nottingham.ac.uk/pathology/protocols/phlox.html

Category: Neuroscience Protocols: Histology Stains Protocols: Bacterial Stains Protocols

Protocol stain for viral inclusions phloxine tartrazine. - [Read Stain for Viral Inclusions Phloxine Tartrazine Protocol]

Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues. - http://www.axis-shield.com/densityhome/optiprep/M17.pdf

Category: Cell Biology and Cell Culture

Summary of the techniques used for the purification of viruses using pre-formed iodixanol gradients. Viruses purified include: Semliki Forest, Retrovirus (from human melanoma cells), Rabies, Polyoma, Papillomavirus, Lassa, Influenza, Bovine viral diarrhea. - [Read Summary of Methods using Pre-formed Iodixanol Gradients for the Purification of Virsues.]

Transient Transfection Protocol: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V2.html

Category: Transfection Protocols: Transient Transfection Protocols

Transient transfection protocol for the ecotropic/amphotropic producer line including titering and concentration of viral stocks. - [Read Transient Transfection Protocol: Viral Production]

Transient Transfection: Viral Production - http://genetics.med.harvard.edu/~cepko/protocol/mike/V1.html

Category: Transfection Protocols: Transient Transfection Protocols

This transient transfection protocol for the ecotropic/amphotropic producer line includes instructions for titering and concentration of viral stocks. This procedure gives consistently higher transfection efficiency. - [Read Transient Transfection: Viral Production]

Viral Detection and Concentration in Food - http://members.ift.org/NR/rdonlyres/69B323CE-B14C-4DD6-8F56-B25898C9EFB0/0/crfsfsv01n2p082085ms20010312.pdf

Category: Viral Protocols

Methods and information related to viral detection and concentration in food. Includes: Nucleic acid amplification methods; Recent progress in polymerase chain reaction detection; Detection of human enteric viruses in non-shellfish food; Choice of primers for the detection of human caliciviruses in foods; Biosensors and microarray detection; Alternative confirmation methods. - [Read Viral Detection and Concentration in Food]

Viral Vector Handling Information - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Category: Cloning Protocols: Vector Protocols

Information on how to handle viral vectors. Includes: Adenovirus; MoMLV retrovirus; MoMLV-based Retroviral Vectors: Base classification BSL-1. - [Read Viral Vector Handling Information]