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Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA Content Protocol - http://cyto.mednet.ucla.edu/Protocols/Simultaneous%20surface%2C%20viability%2C%20DNA.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Immunofluorescence Staining Protocols

Protocol for simultaneous measurement of cell surface immunofluorescence, viability, and DNA content. - [Read Simultaneous Measurement of Cell Surface Immunofluorescence, Viability, and DNA Content Protocol]

SIRC Cytotoxcitiy Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/40.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Cytotoxicity Assays in Cell Culture Protocols

Rabbit-derived corneal cells are cultured in the presence of test compounds, the toxicity of which are determined by their effect upon cell viability. A decrease in cell number, as measured by uptake of the dye Neutral Red, serves as an indicator of potential cytotoxicity. This test has been proposed as a potential replacement alternative for the Draize Eye Irritation test. - [Read SIRC Cytotoxcitiy Test]

Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000182.pdf?pid=PP00000182

Category: Transfection Protocols

The following procedure is for simultaneous transfection and plating of RAW 264.7 cells. This protocol results in approximately 50% to 70% cell viability, and of those viable cells, 20% to 40% are transfected when using pEYFP-N1 from Clontech. Include: Procedure for Splitting Cells before Transfection; Procedure for Preparing Lipofectamine 2000 and DNA; Preparation of RAW 264.7 Cells for Transfection. - [Read Transfecting and Plating RAW 264.7 Cells with Lipofectamine 2000 Protocol]

Trypan blue viability test - http://web.archive.org/web/20030430191647/http://www-ufk.med.uni-rostock.de/lablinks/protocols/e_protocols/trypan.htm

Category: Cell Biology and Cell Culture

Trypan blue viability test for staining dead or dying cells. (Dr.Briese) - [Read Trypan blue viability test]

Viability Cell Count - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-7.html

Category: Cell Biology and Cell Culture

Viability Cell Count. Cell Biology Laboratory Manual. Heidcamp - [Read Viability Cell Count]

Viability Cell Count Protocol - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-7.html

Category: Cell Biology and Cell Culture: Cell Viability Protocols

Protocol for the measurement of viability in cell count. - [Read Viability Cell Count Protocol]

Yeast Plasma Membrane H+ -ATPASE Toxicity Test - http://embryo.ib.amwaw.edu.pl/invittox/prot/34.htm

Category: Pharmacology and Toxicology Protocols: Cytotoxicity Protocols: Microorganism Cytotoxicity Assays Protocols

Accumulation of lipophilic substances, many of which may be environmental chemicals, affects the membrane lipid order and consequently affects the functions of these proteins. Since, the function of important cellular proteins, such as the H+-ATPase strongly depends upon the integrity of the lipid bilayer, the activity of the H+-ATPase may be used as a sensitive indicator of the effect that a chemical may have on the viability of the cell. - [Read Yeast Plasma Membrane H+ -ATPASE Toxicity Test]

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Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants

This protocol describes the electroporation of the BMH 81-17 mut S strain that is recommended for tranformation of the site directed mutagenesis of dsDNA (See Protocol on Site-Directed Mutagenesis on Double Stranded DNA). BMH 81-17 mut S are a mismatch repair defective (mut S) Escherichia coli strain. The probability that the two mutations will cosegregate during the first round of DNA replication is increased in this strain.

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