vesicle Protocols

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Affinity Purification of Antibodies - http://cmgm.stanford.edu/~vesicle/protocols/affinity.html

Category: Protein Protocols: Antibody Purification Protocols

Affinity Purification of Antibodies. Vesicle Trafficking. - [Read Affinity Purification of Antibodies]

Colony Hybridization Protocol - http://cmgm.stanford.edu/~vesicle/protocols/hybrid.html

Category: RNA Protocols: cDNA Libraries Library

Methods and procedure for Colony Hybridization. Vesicle Trafficking Home. - [Read Colony Hybridization Protocol]

GST Fusion Protein Prep - http://cmgm.stanford.edu/~vesicle/protocols/gst_prot.html

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

Detailed protocols and methods for GST protein purification. Vesicle Trafficking, Stanford. - [Read GST Fusion Protein Prep]

Immunohistochemistry on Drosophila Brains Protocol - http://cmgm.stanford.edu/~vesicle/protocols/immunohs.html

Category: Drosophila Protocols: Drosophila Staining Protocols

Protocol for immunohistochemistry on Drosophila brains. - [Read Immunohistochemistry on Drosophila Brains Protocol]

In Situ Hybridization to Tissue Sections Protocol - http://cmgm.stanford.edu/~vesicle/protocols/in_situ.html

Category: Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols

Protocol for in situ hydbridization to tissue sections. Includes: Paraffin Sections; Frozen Sections; Probe preparation; Hybridization and washes; Autoradiography. - [Read In Situ Hybridization to Tissue Sections Protocol]

Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation - http://www.natureprotocols.com/2006/09/26/neuronfibroblast_coculture_ass.php

Category: Neuroscience Protocols: Neuronal Cell Culture Protocols

Neuron-fibroblast coculture assay to demonstrate presynaptic differentiation. Includes: Preparation of HEK293T cells, Coculture of HEK293T cells and neurons, Immunostaining, Vesicle turnover experiment, Image acquisition and quantitation. - [Read Neuron Fibroblast Coculture Assay to Demonstrate Presynaptic Differentiation]

The Cell Attached Capacitance Recording Technique - http://www.natureprotocols.com/2006/11/03/cell_attached_capacitance_meas.php

Category: Neuroscience Protocols: Patch Clamping Protocols

The cell-attached capacitance recording technique is a powerful technique that has been successfully used to resolve single vesicle fusion and fusion pore conductance. This technique, however, has not been applied to synapses because of the difficulty in accessing release sites. Here, we developed a technique to expose release sites in a large nerve terminal, the calyx of Held, which contains clear-core glutamatergic vesicles. - [Read The Cell Attached Capacitance Recording Technique]