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Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4426

Category: Mouse Protocols: Assembling Chimeras Mouse Protocols

Protocol describes a method for producing ES cell-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for ES cell-diploid embryo aggregation in which diploid embryos are replaced with tetraploid embryos. The resulting chimeras can be used to analyze the embryonic versus extraembryonic phenotype of a mutation. - [Read Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos Protocol]

Choosing the Right Labeling Method for your Hybridization Experiment - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_15-18.pdf

Category: Cytogenetics Protocols: In Situ Hybridization Protocols

Choosing the right labeling method for your hybridization experiment. Includes: Homogeneous labeling methods for DNA; Homogeneous labeling methods for RNA; Stability of probe-target interaction; Nonradioactive labeling of oligonucleotides; Double-stranded versus single-stranded probes. - [Read Choosing the Right Labeling Method for your Hybridization Experiment]

Learning and Memory Protocols - http://www.does.org/masterli/ns0805.htm

Category: Neuroscience Protocols

Protocols included: USE OF RADIAL ARM MAZE TASK TO TEST BASIC WORKING MEMORY; USE OF RADIAL ARM MAZE TASK TO TEST WORKING VERSUS REFERENCE MEMORY; USE OF MORRIS WATER MAZE TASK TO TEST SPATIAL MEMORY; USE OF WATER MAZE TASK FOR SPATIAL PROBE TRIAL; USE OF WATER MAZE TASK TO TEST WORKING MEMORY. - [Read Learning and Memory Protocols]

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Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.