Protocol describes castration of male mice, which is used to eliminate testicular hormones and/or to obtain testes for analysis without sacrificing the male. Castration is performed in a similar manner to vasectomy. - [Read Mouse Castration Protocol]
Protocol describes a method for vasectomy in which the vas deferens is accessed through the abdominal wall. Mice are ready for mating after ~10-14 days. Vasectomized males can be bred with fertile females to obtain plugs for timed matings. The pseudopregnant females can then be used for oviduct and uterine transfers. For an alternative protocol, see Vasectomy for Generation of Sterile Males: Access via Scrotal Sac. - [Read Vasectomy for Generation of Sterile Males: Access via Abdominal Wall Protocol]
Protocol describes a method for vasectomy in which the vas deferens is accessed through the scrotal sac. Mice are ready for mating after ~10-14 days. Vasectomized males can be bred with fertile females to obtain plugs for timed matings. The pseudopregnant females can then be used for oviduct and uterine transfers. - [Read Vasectomy for Generation of Sterile Mouse Males: Access via Scrotal Sac Protocols]