variations Protocols

Search results for: variations

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Competitive RT-PCR: Estimation of Copy Number Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4111

Category: PCR Protocols: Reverse Transcriptase RT-PCR Protocols

In this protocol, sample and competitor RNAs are reverse transcribed (separately) in a pilot experiment. A constant amount of sample RT product is then combined with a 2-logserial dilution of competitor RT product for PCR. Procedure provides an approximate copy number for the sample, which is then fine-tuned by repeating the experiment with a series of twofold dilutions of competitor. The experiment includes controls for sample-to-sample variations in RT efficiency. - [Read Competitive RT-PCR: Estimation of Copy Number Protocol]

Fixation Protocol - http://cmif.osu.edu/419.cfm

Category: Histology Protocols: Fixation of Cells Tissues Protocol

There are many variations based on the type of tissue to be examined, whether one is dealing with cell suspensions, biopsies, perfused tissues, or monolayer . Fixation Protocol. OSU Campus Microscopy & Imaging Facility :: The Ohio State University College of Med - [Read Fixation Protocol]

General EMSA Protocol - http://faculty.virginia.edu/mirmira/Protocols/emsa.html

Category: DNA Protocols: EMSA DNA-Protein Protocols

There are multiple variations to this protocol, but they find that this one works well in all cases tested. Mirmira Lab. - [Read General EMSA Protocol]

The Basic Polymerase Chain Reaction Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3824

Category: PCR Protocols: Standard PCR Protocol

Protocol describes how to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase. It is the foundation for all subsequent variations of the polymerase chain reaction. - [Read The Basic Polymerase Chain Reaction Protocol]

Yeast Chromatin Immunoprecipitation (ChIP) Assay Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4642

Category: Yeast Protocols: Yeast Protein Protocols: Yeast Immunoprecipitation Protocols

Protocol describes a method for the detection of proteins bound to specific regions of chromatin in yeast. There are many variations of this assay. - [Read Yeast Chromatin Immunoprecipitation (ChIP) Assay Protocol]