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5'-End cDNA Amplification Using New RACE Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4132

Category: PCR Protocols: cDNA Synthesis Protocols

New RACE, a variation of RNA ligase-mediated-RACE (RLM-RACE) (Liu and Gorovsky 1993) departs from classic RACE (see 5'-End cDNA Amplification Using Classic RACE) in that an "anchor" primer is attached to the 5'-end of the mRNA before the reverse transcription step; hence the anchor sequence becomes incorporated into the first-strand cDNA if, and only if, the reverse transcription proceeds through the entire length of the mRNA of interest. - [Read 5'-End cDNA Amplification Using New RACE Protocol]

AFLP's - http://opbs.okstate.edu/~melcher/mg/mgw1/MG11128.html

Category: PCR Protocols: AFLP PCR

AFLP is a variation of RAPD. Facts of AFLP PCR. Ulrich Melcher. - [Read AFLP's]

DNA extraction from blood - http://genome-lab.ucdavis.edu/Protocols/dna_extraction_from_blood.htm

Category: DNA Protocols: DNA Extraction Blood

Purifying nuclear pellets, Extraction of DNA from nuclei. Method for DNA isolation from Blood. Genomic Variation Laboratory, UC Davis. - [Read DNA extraction from blood]

DNA Transfection Mediated by Lipofection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3870

Category: Transfection Protocols

Protocol should be viewed as a starting point for systematic optimization of transfection mediated by lipofecting agents. Once a positive signal has been obtained from a transfected plasmid carrying a standard reporter gene, optimal conditions for transfection can be established by systematic variation of parameters such as the initial cell density, the amount and purity of DNA, the media and serum, and the time of exposure of the cells to the cationic-lipid-DNA complex. - [Read DNA Transfection Mediated by Lipofection Protocol]

PCR Tips and Tricks - http://genome-lab.ucdavis.edu/Protocols/pcr_tips/pcr_tips_and_tricks.htm

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

PCR Tips and Tricks - http://genome-lab.ucdavis.edu/Protocols/pcr_tips/pcr_tips_and_tricks.htm

Category: PCR Protocols: PCR Optimization

PCR Tips and Tricks - General Guidelines and Troubleshooting PCR polymerase chain reaction. The Genomic Variation Laboratory, U.C Davis - [Read PCR Tips and Tricks]

Articles (1-1 of 1)

Vector Design Protocol for Transgenic Mice Generation

The protocol gives general considerations for the design of targeting vectors for transgenic mice. The protocol shares tips in the design of knock-out and knock-in vectors and some of their strategies for producing homologously recombined embryonic stem cells.