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Polycrystalline thin films Sample Preparation Protocol MALDI - http://prowl.rockefeller.edu/methods/thinfilm.htm

This method of sample preparation produces a uniform layer of very small crystals on the mass spectrometer's sample stage that are mechanically well adhered to the substrate. It is a variant of the dried droplet method. PROWL - [Read Polycrystalline thin films Sample Preparation Protocol MALDI]

Probing Protein Interactions Using GFP and FRET - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3822

Category: Reporter Gene Assays: GFP Assay Protocols

Protocol specifically describes data acquisition for a particular variant of GFP (EGFP) or Oregon Green as a donor fluorophore, but it can be adapted for image acquisition of other chromophore. - [Read Probing Protein Interactions Using GFP and FRET]

Probing Protein Interactions Using GFP and FRET Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3893

Category: Reporter Gene Assays: GFP Assay Protocols

In preparation for FLIM-FRET analysis, the appropriate donor and acceptor components must be introduced into live or fixed cells. The method of introduction depends on the nature of the components and the state of the cells. For example, plasmid DNAs encoding a protein of interest fused to a variant of GFP may be introduced into live cells by transfection or microinjection, whereas labeled antibodies are delivered by microinjection. - [Read Probing Protein Interactions Using GFP and FRET Protocol]

Whole-Cell Voltage Clamp of Isolated Heart Cells - http://www.southalabama.edu/ishr/help/wholecellclamp/

Category: Neuroscience Protocols: Patch Clamping Protocols

This article briefly summarizes the use of this variant of the patch-clamp technique in isolated cardiac myocytes with an emphasis on practical suggestions. - [Read Whole-Cell Voltage Clamp of Isolated Heart Cells]