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Construction of Genomic DNA Libraries in Cosmid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4000

Category: DNA Protocols: Cosmid Library Protocols

Protocol describes how to construct a library of 35-45-kb fragments of genomic DNA in the double cos site cosmid vector, SuperCos-1. The steps include: Linearization and dephosphorylation of SuperCos-1 DNA; Partial digestion of high-molecular-weight DNA with MboI; Dephosphorylation of high-molecular-weight genomic DNA; Ligation of cosmid arms to genomic DNA: Packaging and plating recombinants; Isolation and analysis of recombinant cosmids: Validation of the library. - [Read Construction of Genomic DNA Libraries in Cosmid Vectors Protocol]

DNA Microarray Procedures - http://omrf.ouhsc.edu/~frank/marraytraining.html

Category: DNA Microarray Protocols

Protocols, Frequently Asked Questions, Validation. Dr. Bart Frank - [Read DNA Microarray Procedures]

Estimating the Number of CD38 Molecules on the CD8+ T Lymphocytes of HIV-Infected Individuals - http://cyto.mednet.ucla.edu/Protocols/cd38.pdf

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Protocol for estimating the number of CD38 molecules on the CD8+ T lymphocytes of HIV-infected individuals. Includes: RECOMMENDATION OF VENDOR FOR PE-CD38 AND PE-CD4; VALIDATION OF LOGARITHMIC AMPLIFIER LINEARITY AND SENSITIVITY; CONSERVATION OF THE LEVEL OF CD4 ANTIGEN EXPRESSION ON CD4+ LYMPHOCYTES AND ITS USE AS A BIOLOGIC STANDARD FOR FLOW CYTOMETER INSTRUMENT CHARACTERIZATION; DETERMINATION OF THE NUMBER OF CD38 MOLECULES PER CD8+ CELL; etc.. - [Read Estimating the Number of CD38 Molecules on the CD8+ T Lymphocytes of HIV-Infected Individuals]

Paternity Testing, Forensic DNA Testing & Mouse Genotyping Careers - Identigene - http://www.identigene.com/SWIMX/about/careers.asp

Category: Molecular Model Organisms: Mouse Animal Protocols: Mouse Genotyping Protocols

IDENTIGENE is looking for a Scientist to help expand our Custom Mouse ... Protocol validation; Sample genotyping; Scale-up of laboratory processes for ... - [Read Paternity Testing, Forensic DNA Testing & Mouse Genotyping Careers - Identigene]

Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT - http://omrf.ouhsc.edu/~frank/M_QPCR-OMNISCRIPT-RT.html

Category: Real Time PCR

Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT. Dr. Craig Cadwell - [Read Validation of Microarray Results Using Quantitative Real-Time PCR (QRT PCR) with Omniscript RT]