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AFLP protocol Wolf Lab - http://bioweb.usu.edu/wolf/aflp_protocol.htm

Restriction and Ligation reactions, Setting up the reactions, AFLP PCR reactions protocols. Analyzing the data using Genescan. Paul G. Wolf, Utah State Univ. - [Read AFLP protocol Wolf Lab]

Bielschowsky Technique for Senile Plaques Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/BIELSCH.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

A silver stain to demonstrate neurofibrillary tangles, nerve fibers and senile plaques in Alzheimer's disease. The nerve fibers are sensitized with a silver solution. The sections are treated with ammoniacal silver, and then reduced to a visible metallic silver. - [Read Bielschowsky Technique for Senile Plaques Protocol]

Congo Red Putcher's Modification Amyloid Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/CONGORED.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol to demonstrate amyloid deposits in tissue sections. When stained with the Congo Red Stain the amyloid, with the aide of polarizing lenses, will birefringe an apple green color. Under the microscope. - [Read Congo Red Putcher's Modification Amyloid Protocol]

Histotechniques - Manual - http://medlib.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html

Category: Histology Protocols

Histotechniques - Manual. Great Histology Protocol Handbook. Includes: Tissue Processing, Specimen Accessioning, Fixation, Fixatives, Tissue Processing, Sectioning, H and E staining, more. - [Read Histotechniques - Manual]

Histotechniques Fixation - http://library.med.utah.edu/WebPath/HISTHTML/HISTOTCH/HISTOTCH.html

Category: Immunohistochemistry Protocols: Fixation Protocols

Information on histotechniques. Includes: Fixation - types of fixatives; factors affecting fixation; General usage of fixatives; Tissue Processing; Sectioning; Frozen Sections; Staining; H and E staining; Coverslipping; Decalcification; Artefacts in Histologic Sections; Problems in Tissue Processing. - [Read Histotechniques Fixation]

Holzer's Stain Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/HOLZER.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols

An appropriate term for glial fibers is 'nerve glue', because they provide the internal support of the central nervous system. There are four types of glial cells: astrocytes, oligosendroglia,microglia, and ependymal cells. The glia fibers are stained with crystal violet which are resistant to the aniline-chloroform differentiating solution. - [Read Holzer's Stain Protocol]

Modified Thioflavin for Senile Plaques Amyloid Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/MODTHIOF.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol for modified thioflavin for senile plaques amyloid. - [Read Modified Thioflavin for Senile Plaques Amyloid Protocol]

Neuroanatomy Tutorial - http://library.med.utah.edu/WebPath/HISTHTML/NEURANAT/NEURANCA.html

Category: Neuroscience Protocols

This tutorial has images in which the structures are labelled. You are to identify the structures by clicking on the name of the structure. - [Read Neuroanatomy Tutorial]

Special Stains in Histology - http://medlib.med.utah.edu/WebPath/HISTHTML/STAINS/STAINS.html

Category: Histology Protocols

Special Stains in Histology. Mucin stains, Melanin stains, etc. - [Read Special Stains in Histology]

Staining for Myelin Sheath Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/LFB.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protocol for myelin sheath. Luxol Fast Blue is the alcohol soluble counterpart of the water soluble Alcian Blue. Staining is due to lipoproteins, and the mechanism is one of an acid-base reaction with salt formation; the base of the lipoprotein replaces the base of the dye. - [Read Staining for Myelin Sheath Protocol]

Staining Manual and Fixing Manual. - http://medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html

Category: Histology Protocols: Histological Fixation

95% Alcoholic Bouin's Fixative, B5 Fixative, Formalin Fixative, Zenker's Fixative. Susan L. Wall Utah. - [Read Staining Manual and Fixing Manual.]

Staining of Nerve Fibers Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/BODIAN.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols

Protargol-S (silver proteinate) is used with the addition of copper metal. The copper replaces the silver in the connective tissue, allowing a greater differentiation between the nerve fibers and the connective tissue. The silver is reduced with hydroquinone to the visible metallic form. The sections are toned with gold chloride, the gold chloride is reduced with oxalic acid, increasing the deposit of metallic gold on the sections. - [Read Staining of Nerve Fibers Protocol]

Staining Procedures for Histology - http://medlib.med.utah.edu/WebPath/HISTHTML/MANUALS/MANUALS.html

Category: Histology Protocols: Histology Staining Procedures

Acid Fast Stain, Colloidal stain, Copper Stain, Congo Red Stain, Giemsa, Methyl Green Pyronin (MGP) Stain, Oil Red O Stain, many others. Internet Pathology Laboratory for Medical Education, Florida State. - [Read Staining Procedures for Histology]

Thioflavine S Amyloid Protocol - http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/THIOFLAV.PDF

Category: Neuroscience Protocols: Neuronal Stains Protocols: Stains for Senile Plaques Protocols

Protocol for thioflavine amyloid. - [Read Thioflavine S Amyloid Protocol]