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COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL - http://users.ugent.be/~fspelema/neubla/protocol/cgh.htm

Category: Cytogenetics Protocols: Comparative Genomic Hybridization CGH Protocols

SAVING OF DAPI-IMAGES, SLIDE PRETREATMENT, PROBE PREPARATION, PROBE DETECTION, washing blocking detection, Counterstaining with DAPI. Institute of Pathology,Humboldt-University of Berlin - [Read COMPARATIVE GENOMIC HYBRIDIZATION (CGH) PROTOCOL]

Electroporation of Cell Lines With DNA - http://users.path.ox.ac.uk/~ciu/Methods/DNA/electroporation.html

Category: Cell Biology and Cell Culture: Cell Electroporation Protocols

Electroporation of Cell Lines With DNA. Electroporation for the efficient transfection of mammalian cells with DNA. Chu et al. Cellular Immunology Oxford - [Read Electroporation of Cell Lines With DNA]

Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol - http://users.ugent.be/~fspelema/neubla/protocol/fish.htm

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for fluorescence in situ hybridization (FISH) on human chromosomes and interphase nuclei. Includes: Plasmid probes; COSMID-, YAC- and LIBRARY PROBES; Probe detection. - [Read Fluorescence In Situ Hybridization (FISH) on Human Chromosomes and Interphase Nuclei Protocol]

Footprinting Protocol - http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/F/Footprinting.html

Category: DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols

Protocol for footprinting. - [Read Footprinting Protocol]

FPLC Protocol - http://users.path.ox.ac.uk/~ciu/Methods/Protein/fplc.html

Category: Chromatography Protocols

FPLC Protocol. The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. For use of individual columns follow the "instructions" (in the green folder) which accompany each one. Sir William Dunn School of Pathology, Oxford University. - [Read FPLC Protocol]

Large Scale Production of Monoclonal Antibodies - http://users.path.ox.ac.uk/~scobbold/tig/mprod.html

Category: Antibody Protocols: Antibody Production Protocols

Large scale production of monoclonal antibodies. Includes: Strategies to minimize mAb production costs; A home-made hollow fibre bioreactor system. - [Read Large Scale Production of Monoclonal Antibodies]

Laser-capture Microdissection Protocol - http://www.nature.com/nprot/journal/v1/n2/full/nprot.2006.85.html#a1

Category: Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols

LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. A variety of downstream applications exist: DNA genotyping and loss-of-heterozygosity (LOH) analysis, etc. Protocol provides a thorough description of LCM techniques, with an emphasis on tips and troubleshooting advice derived from LCM users. The total time required to carry out this protocol is typically 1–1.5 h. - [Read Laser-capture Microdissection Protocol]

Preparation of Stock Solutions for FISH Protocol - http://users.ugent.be/~fspelema/neubla/protocol/stock.htm

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Protocol for the preparation of stock solutions for FISH. - [Read Preparation of Stock Solutions for FISH Protocol]

Radiation Safety Manual - http://www.cdc.gov/OD/ohs/manual/radman.htm

Category: Radioactivity: Radioaction Safety

Administration of the Radiation Safety Program, , Physical Security of Radioactive Materials, "As Low as Reasonably Achievable" (ALARA) Philosophy, Authorized Users, Radiation Workers , Permitted Workers, Training, Procurement, Inventory and Records, Personnel Monitoring, Radiation Exposure During Pregnancy, Laboratory Supplies and Equipment, Bioassay, Labeling, Surveys, Radioactive Waste Disposal. U.S. Department of Health and Human Services, CDC, Atlanta, GA. - [Read Radiation Safety Manual]

SDS-PAGE An Introduction - http://www.bio.davidson.edu/courses/genomics/method/SDSPAGE/SDSPAGE.html

Category: Protein Protocols: Protein Electrophoresis

SDS-PAGE An Introduction. A great introduction to SDS-PAGE for first time users. - [Read SDS-PAGE An Introduction]

Articles (1-1 of 1)

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.