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Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Accumulation and Fixation of Plant Metaphase Chromosomes Protocol - http://www.le.ac.uk/bl/phh4/proaccum.htm

Category: Plant Biology Protocols: Plant Genetics Protocols

For analysis of metaphase chromosomes, any tissue containing dividing cells can be used: Root tips from young seedlings, from newly grown roots at the edge of plant pots or hydroponic culture are all suitable. Alternatively, flower buds, anthers, carpels or leaf or apical meristems can be used. Includes metaphase arresting reagents. - [Read Accumulation and Fixation of Plant Metaphase Chromosomes Protocol]

Acetone precipitation of protein from Buffer RLT or Buffer RLT - http://www1.qiagen.com/literature/protocols/pdf/RY22.pdf

Category: Protein Protocols: Protein Precipitation Procols

Acetone precipitation of protein from Buffer RLT or Buffer RLT. QIAGEN. - [Read Acetone precipitation of protein from Buffer RLT or Buffer RLT]

Acetyl Cholinesterase Enzyme Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/cholase.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol stain for acetyl cholinesterase enzyme. - [Read Acetyl Cholinesterase Enzyme Histochemical Stain Protocol]

aCGH Labeling Protocol - http://genomic.dfci.harvard.edu/aCGHLabelingChecklistv1_9.pdf

Category: Cytogenetics Protocols: Array CGH

aCGH Labeling Protocol. Harvard University. - [Read aCGH Labeling Protocol]

Acid Cleaning Coverslips Protocol - http://spectorlab.cshl.edu/acid_clean.html

Category: Immunohistochemistry Protocols: Coated Treated Slides Protocols

Protocol for acid cleaning coverslips. - [Read Acid Cleaning Coverslips Protocol]

Acid Phosphatase Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/acphos.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for acid phosphatase histochemical stain. - [Read Acid Phosphatase Histochemical Stain Protocol]

Acid Picro Mallory Connective Tissue Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/acidpicro.html

Category: Neuroscience Protocols: Histology Stains Protocols: Connective Tissue Stains Protocols

Protocol for connective tissue stain. Acid Picro Mallory. - [Read Acid Picro Mallory Connective Tissue Stain Protocol]

Acrylamide Gels for Resolving Small (25bp-700bp) DNA Fragments Protocol - http://www.oardc.ohio-state.edu/stockingerlab/Protocols/AcrylamideGels%28DNA%29.pdf

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols

Protocol for acrylamide gels for resolving small (25bp-700bp) DNA fragments. - [Read Acrylamide Gels for Resolving Small (25bp-700bp) DNA Fragments Protocol]

Actin Binding & Bundling Sedimentation Assays - http://www2.bc.edu/~burgesda/protocols/Actin_Binding.html

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Protocol includes: Binding assays for tropomyosin, villin or caldesmon and Bundling Assays - [Read Actin Binding & Bundling Sedimentation Assays]

Actin Capture Assay Protocol - http://genomics.princeton.edu/botstein/protocols/actin_capture.html

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Protocol for actin capture assay. Includes: Actin Capture Assay Reagants. - [Read Actin Capture Assay Protocol]

Actin Capture Assay Protocol - http://genomics.princeton.edu/botstein/protocols/actin_capture.html

Category: Yeast Protocols

Protocol for actin capture assay. - [Read Actin Capture Assay Protocol]

Actin Staining in Fixed Yeast Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4168

Category: Cytoskeleton Protocols: Actin Myosin Protocols

Phalloidin binds specifically to F-actin, and fluorescent-tagged phalloidin stains the actin skeleton in cells in a manner that is very close to the staining pattern seen using anti-actin antibody. - [Read Actin Staining in Fixed Yeast Cells Protocol]

Activated MAP Kinase Protocols Stratagene PDF - http://www.stratagene.com/manuals/206110.pdf

Category: Signalling Signal Transduction Protocols: MAPK Protocols

MAPK Protocols. Resolving Phosphorylated MAPK Proteins by SDSâ€“PAGE, Filter Binding Assay for activated MAPK Î³-32P incorporation of MAPK. Stratagene - [Read Activated MAP Kinase Protocols Stratagene PDF]

Activation of p70s6k and MAP Kinases: IP and Kinase Activity - http://www.whitelabs.org/Lab%20Protocols/kinase%20and%20phosphatase%20assays/ACTIVATION%20OF%20p70s6k%20and%20MAP%20KINASES.htm

Category: Signalling Signal Transduction Protocols: MAPK Protocols

Protocol describes the activation of MAP Kinase activity. Protocol contains information on: Buffers: ST, STE, Lysis Buffer Buffer A, Buffer B and 10x Kinase Buffer. - [Read Activation of p70s6k and MAP Kinases: IP and Kinase Activity]

Adaptation of High Five™ Cells to HyQ© SFX Insect Medium - http://www.hyclone.com/pdf/procedure_insect_highfive.pdf

Category: Cell Culture Protocols: Insect Cell Culture Protocols

This protocol is designed to adapt High Five™ (BTI-Tn-4) cells to serum-free suspension culture in HyQ SFX-Insect in 5 passages. The entire adaptation process takes approximately two weeks and the resulting adapted cells have doubling times of 16 and 20 hours and minimal clumping in suspension culture. - [Read Adaptation of High Five™ Cells to HyQ© SFX Insect Medium]

Adapting Cells to a Serum-Free Environment Protocol - https://fscimage.fishersci.com/webimages_FSC/downloads/HyClone_Protocol_8.pdf

Category: Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols

There are many ways to adapt cell lines to serum-free media. Five methods are presented that are designed for adapting hybridomas to a protein-free medium. These protocols may require some modifications for your particular cell line and conditions. - [Read Adapting Cells to a Serum-Free Environment Protocol]

Addition of linkers/adaptors to blunt-end DNA fragments PDF - http://www.oup.co.uk/pdf/pas/9v1-7-11.pdf

Category: Molecular Biology Protocols: DNA Ligation Protocols

Addition of linkers/adaptors to blunt-end DNA fragments. Ligation of mixture containing DNA and linkers/adaptors. Powell Gannon. Oxford Practical Approach Series. - [Read Addition of linkers/adaptors to blunt-end DNA fragments PDF]

Additional slide pre-treatment for mapping smaller insert clones - http://www.sanger.ac.uk/HGP/methods/cytogenetics/additional_treatment.shtml

Category: Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol

Additional slide pre-treatment for mapping smaller insert clones for FISH. Sanger Institute. - [Read Additional slide pre-treatment for mapping smaller insert clones]

Adenosine Triphosphatase ATPASE Histochemical Stain Protocol - http://www.nottingham.ac.uk/pathology/protocols/atpase.html

Category: Neuroscience Protocols: Histology Stains Protocols: Enzyme Histochemical Stains Protocols

Protocol for adenosine triphosphatase ATPASE histochemical stain. - [Read Adenosine Triphosphatase ATPASE Histochemical Stain Protocol]

Adenovirus DNA Replication Assay Protocol - http://ruummc.med.uu.nl/subsite/RepHTML/Protocols/AdDNAreplication.html

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol describes large fragment DNA replication by Adenovirus proteins using TP-DNA as template. - [Read Adenovirus DNA Replication Assay Protocol]

Adenovirus Protocol - http://www.mc.vanderbilt.edu/root/vumc.php?site=mgcore&doc=4301

Category: Viral Culture and Isolation Protocols: Viral Isolation Protocols

Protocol for Adenovirus. - [Read Adenovirus Protocol]

Adherent Cell Types Infection Protocol - http://www.med.umich.edu/vcore/protocols/RetroviralCellScreenInfection101705.pdf

Category: Cloning Protocols: Vector Protocols

Protocol for infection for adherent cell types. - [Read Adherent Cell Types Infection Protocol]

Advanced Methodologies in Flow Cytometry - http://www.cyto.purdue.edu/flowcyt/research/cytotech/amfc/data/index1.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols: Flow Cytometry Data Analysis Protocols

Advanced Methodologies in Flow Cytometry. Andrea Cossarizza et al.. Materials from a workshop held March, 1997 in Modena, Italy. - [Read Advanced Methodologies in Flow Cytometry]

Advances in Cytochemical Methods for Detection of Apoptosis - http://www.jhc.org/cgi/content/full/49/7/821

Category: Cell Biology and Cell Culture: Apoptosis Protocols

Advances in Cytochemical Methods for Detection of Apoptosis. Katherine L. Barrett et al. 2001 - [Read Advances in Cytochemical Methods for Detection of Apoptosis]

Affinity Antibody Purification of Protein A/G Chromatography - http://www.upstate.com/misc/protocol_detail.q.prot.e.antibody_purification.a.name.e.Antibody_Purification

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

Antibody Purification (Antiserum or Ascites by Protein A/G Chromatography). Species and Type of Antibody Agarose Rabbit IgG Protein A or Protein G Mouse IgG1 Protein G Mouse IgG2 Protein A or Protein G Mouse IgG3 Protein G Sheep IgG Protein G but binds weakly Rat IgG Protein G but binds weakly Guinea Pig IgG Protein A Dog IgG Protein A Goat IgG Protein G Pig IgG Protein A Hamster IgG Protein G. By Millipore. - [Read Affinity Antibody Purification of Protein A/G Chromatography]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.