urea Protocols

Search results for: urea

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4549

Category: Chromatography Protocols: Liquid Chromatography Protocols

Protocol for desalting of peptides and protein mixtures by RP-HPLC techniques. The RP-HPLC technique can be used to "desalt" peptide or protein samples derived from extraction procedures, from chemical reactions such as reductive alkylation in the presence of urea or guanidine hydrochloride, citraconylation, iodination, or cyanogen bromide cleavage, or recovered from other chromatographic separation. - [Read Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol]

General Immunofluorescence Protocol - http://www.bcm.edu/rosenlab/protocols/generalIF.pdf

Category: Molecular Imaging: Immunofluorescence Microscopy

General Immunofluorescence Protocol. Rosen Lab. Deparaffinize and rehydrate sections, Antigen retrieval methods, Proteinase K Antigen Retrieval, Urea Antigen Retrieval, Blocking buffers, - [Read General Immunofluorescence Protocol]

General Immunohistochemistry Protocol - http://www.bcm.edu/rosenlab/protocols/generalIHC.pdf

Category: Immunohistochemistry Protocols

Protocol for general immunohistochemistry. Includes: Antigen retrieval methods; Sodium Citrate Antigen Retrieval; Proteinase K Antigen Retrieval; Urea Antigen Retrieval. - [Read General Immunohistochemistry Protocol]

Protocol for Polymerase III In Vitro Transcription - http://www.bio.com/protocolstools/protocol.jhtml?id=p1140

Category: Cell Biology and Cell Culture: In Vitro Reconstitution Protocols

Protocol describes an RNA Polymerase III (Pol III) transcription assay using an extract or proteins of choice. Pol III is the polymerase responsible for transcribing 5S RNA, tRNAs, and other small RNAs. Î±-Amanitin inhibits Pol II transcription in the assay. The newly-transcribed, radiolabeled RNA is visualized by autoradiography following Urea Polyacrylamide gel electrophoresis. - [Read Protocol for Polymerase III In Vitro Transcription]

Purification of Histidine-Tagged Proteins under Denaturing Conditions Using IMAC Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4221

Category: Chromatography Protocols: Immobilized Metal Ion Affinity Chromatography Protocols

Immobilized metal-ion affinity chromatography (IMAC) is suitable for the purification of proteins under denaturing conditions. Either guanidine-HCl or urea can be used, although guanidine-HCl is a stronger denaturant than urea. Proteins that have been adsorbed to the column in the presence of guanidine-binding buffer may be washed with urea-binding buffer and eluted with urea elution buffer. - [Read Purification of Histidine-Tagged Proteins under Denaturing Conditions Using IMAC Protocol]