upper Protocols

Search results for: upper

Protocols » Search Results

Search Results Protocol Links Sort by: Hits | Alphabetical

Preparation of Electrolyte Gradient Gels Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3795

Category: Nucleic Acid Electrophoresis Protocols

Protocol for the preparation of electrolyte gradient gels. Electrolyte gradients are formed when buffers of different concentrations are used in the upper (low electrolyte concentration) and lower (high electrolyte concentration) chambers of the electrophoresis device. - [Read Preparation of Electrolyte Gradient Gels Protocol]

Purification of Demethylated Sphingomyelin Protocol - http://www.duke.edu/web/ceramide/protocols/0032.html

Category: Lipid Protocols

Protocol for the purificiation of demethylated sphingomyelin. Includes: Lower, chloroform phase; Upper, aqueous phase and interphase. - [Read Purification of Demethylated Sphingomyelin Protocol]

TransWell Chemotaxis Protocol. - http://bioprotocol.bio.com/protocolstools/protocol.jhtml?id=p2156

Category: Immunology: Chemotaxis

TransWell Chemotaxis protocol. trans-well chemotaxis method from bioprotocol. The protocol is a method for studying migration towards a concentration gradient of chemoattractant of leukocytes (neutrophils, monocytes and lymphocytes) or other migratory cells. An upper chamber containing a suspension of cells is separated by a membrane from a lower chamber containing medium with chemoattractant. Chemotaxis of the cells from the upper chamber into the lower chamber can be quantified. - [Read TransWell Chemotaxis Protocol.]

Articles (1-2 of 2)

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Acid Guanidinium Thiocyanate RNA Isolation Phenol Chloroform Extraction

A single step RNA isolation protocol using Phenol Chloroform Extraction and Acid Guanidinium Thiocyanate. This RNA isolation method uses the fact that guanidinium thiocyanate can simultaneously lyse the cells and inactive cellular RNAses during the initial RNA isolation step allow a single step in the method.

[1-2]