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RNA Band Shift Assay. EMSA. - http://www.biochem.uiowa.edu/donelson/Database%20items/rnabndshft.htm

Category: RNA Protocols: RNA-binding Protein EMSA Interactions

Donelson Lab, University of Iowa. - [Read RNA Band Shift Assay. EMSA.]

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) - http://www.mcb.uct.ac.za//sdspage.htm

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE). Ed Rybicki and Maud Purves. Dept Microbiology, University of Cape Town. PROTOCOL includes: Assembling Gel Apparatus, Resolving Gels, Stacking Gels, Electrophoresis buffer, Sample Preparation for Sds-page and Staining of Gels. - [Read SDS POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE)]

SECTIONING RESIN EMBEDDED TISSUE - http://www.nottingham.ac.uk/pathology/protocols/resinsect.html

Category: Histology Protocols: Tissue Sample Sectioning

SECTIONING RESIN EMBEDDED TISSUE. MAKING LATTA-HARTMANN KNIVES and Sectioning Protocols for Histology. University of Nottingham Medical School Division of Histopathology. James Lowe. - [Read SECTIONING RESIN EMBEDDED TISSUE]

Serial Analysis Of Gene Expression (SAGE) - http://www.embl-heidelberg.de/info/sage/

Category: Genetics and Genomics: Cancer Genetics Protocols

SAGE is a new method that has been invented at Johns Hopkins University in USA to give scientists an overview of a cell’s complete gene activity. It works by capturing RNAs, identifying them and counting them. By comparing different types of cells, the researchers hope to generate profiles that will help them understand healthy cells and what goes wrong during diseases. Includes: How SAGE works and Steps of SAGE. - [Read Serial Analysis Of Gene Expression (SAGE)]

Simple RT-PCR Protocol - http://preuss.bsd.uchicago.edu/protocols/RT.html

Category: RNA Protocols: RT-PCR and cDNA synthesis

Simple RT-PCR Protocol. University of Chicago The Preuss Lab - [Read Simple RT-PCR Protocol]

Simplified Arabidopsis Transformation Protocol - http://plantpath.wisc.edu/~afb/protocol.html

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Agrobacterium tumefaciens strain transformation of arabidopsis thaliana. Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. - [Read Simplified Arabidopsis Transformation Protocol]

Test Knowing How to Practice Safe Science - http://info.med.yale.edu/caim/hhmi/public/

Category: General Research Protocols and Methods: Laboratory Safety

Test Knowing How to Practice Safe Science. Yale University. A great online interactive test for lab safety. - [Read Test Knowing How to Practice Safe Science]

The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function - http://pen2.igc.gulbenkian.pt/cftr/vr/d/hug_whole_cell_patch_clamp_tool_cftr_function.pdf

Category: Electrophysiology Ion Channels

The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function. Martin J. Hug. University of Muenster, Institute of Physiology. Germany - [Read The Whole-Cell Patch-Clamp Technique â€“ a powerful tool to approach CFTR function]

Timeline for Transgenic Mouse Analysis - http://www.med.umich.edu/tamc/tgtime.html

Category: Transgenic Mouse Protocols

Timeline for Transgenic Mouse Analysis and production. University of Michigan Transgenic Animal Model Core. Transgenic Animal Web. - [Read Timeline for Transgenic Mouse Analysis]

Transgenic Mouse Breeding - http://www.med.umich.edu/tamc/breed.html

Category: Transgenic Mouse Protocols

Mouse Breeding Suggestions, ES Cell-Mouse Chimera Breeding, Record Keeping, Transmission Patterns. University of Michigan Transgenic Animal (Web) Model Core - [Read Transgenic Mouse Breeding]

USE OF THE LIGHT MICROSCOPE - http://www.bristol.ac.uk/vetpath/cpl/critillum.html

Category: Molecular Imaging: Light Microscopy

USE OF THE LIGHT MICROSCOPE. Step by step methodology guide on how to use a light microscope. Veterinary Pathology. Bristol University - [Read USE OF THE LIGHT MICROSCOPE]

Yeast and Bacterial Colony PCR Protocol. - http://www.upstate.edu/biochem/amberg/protocols/colony_pcr.html

Category: PCR Protocols: Colony PCR

Yeast and Bacterial Colony PCR Protocol. David Amberg SUNY Upstate Medical University - [Read Yeast and Bacterial Colony PCR Protocol.]

Yeast Genomic DNA Isolation Procedure - http://www.upstate.edu/biochem/amberg/protocols/yeast_genomic_DNA.html

Category: DNA Protocols: DNA Extraction Protocols

Yeast Genomic DNA Isolation David Amberg, Upstate Medical University. - [Read Yeast Genomic DNA Isolation Procedure]

Yeast Immunofluorescence with methanol - http://www.upstate.edu/biochem/amberg/protocols/MeOH_IF.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Yeast IF with Methanol/Acetone Dehydration. Amberg Lab, Upstate Medical University. - [Read Yeast Immunofluorescence with methanol]

Zero Blunt TOPO PCR Cloning Kit Protocol - http://www.hawaii.edu/microbiology/MO/topo.htm

Category: DNA Protocols: TOPO Cloning

Zero Blunt TOPO PCR Cloning Kit, Detailed protocol. (Invitrogen Life technologies). Alam Lab. Department of Microbiology, University of Hawai'i. - [Read Zero Blunt TOPO PCR Cloning Kit Protocol]

Articles (1-4 of 4)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

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