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Laboratory Safety Program - http://www.uwm.edu/Dept/EHSRM/LAB/index.html

Category: General Research Protocols and Methods: Laboratory Safety

Laboratory Safety Program - Emergency Preparedness, Autoclave Safety, Biosafety, Carcinogens, Chemical Hygiene Plan, Glass Disposal, Fire Safety, Eyewash and Safety Shower Information, Fume Hood Procedures and Practices. University of Wisconsin - Milwaukee. - [Read Laboratory Safety Program]

Ligation with GUS vector and Transformation - http://www.owlnet.rice.edu/~bios311/bios311/bios312/312day3.html

Category: Molecular Biology Protocols: DNA Ligation Protocols

Ligation with GUS vector and Transformation. Chemical Transformation of E. coli. Ligation Procedure for LigaFastâ„¢ Rapid DNA Ligation System (Promega). B. Beason, Rice University. - [Read Ligation with GUS vector and Transformation]

Light Microscopy - http://www.ruf.rice.edu/%7Ebioslabs/methods/microscopy/microscopy.html

Category: Molecular Imaging: Light Microscopy

Types of light microscopy, Bright Field Microscopy, Using a bright field microscope, mounting on slides, adjusting the microscope, Care of the microscope, When to use bright field microscopy. David R. Caprette. Rice University. - [Read Light Microscopy]

List of standard EM Center procedures/protocols - http://anatomy.iupui.edu/emcenter/protocols.html

Category: Molecular Imaging: Electron Microscopy Protocols

Protocol for sectioning is on a separate page. 2. Standard Procedure To Section for Transmission Electron Microscopy, EM Center, Indiana University School ... List of standard EM Center procedures/protocols - [Read List of standard EM Center procedures/protocols]

Lowry â€“ Protein Determination - http://wolfson.huji.ac.il/purification/Protocols/loury.html

Category: Protein Protocols: Lowry Protein Assay

Lowry â€“ Protein Determination. Quantitation of total protein using the lowry protein assay. Dr. Mario Lebendiker. The Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem. Method materials and notes for the Lowry assay. - [Read Lowry â€“ Protein Determination]

Lowry Protein Assay - http://www.animal.ufl.edu/hansen/protocols/LOWRY.htm

Category: Protein Protocols: Lowry Protein Assay

Lowry Protein Assay. The Lowry procedure is one of the most venerable and widely-used protein assays, being first described in 1951 [Lowry et al., J. Biol. Chem. 193: 265-275 (1951)]. Under alkaline conditions, copper complexes with protein. When folin phenol reagent (phospho-molybdic-phosphotungstic reagent) is added, the Folin-phenol reagent binds to the protein. Bound reagent is slowly reduced and changes color from yellow to blue. P.J. Hansen, Dept. of Animal Sciences, University of Florida. - [Read Lowry Protein Assay]

Luciferase Assay Protocol for HEK COS cells - http://www.ucc.ie/ucc/depts/physio/MolPhysiol/MolPhys/Protocols/pdfs/012.pdf

Category: Luciferase and Dual Luciferase Assays

Luciferase Assay Protocol for HEK COS cells. University College Cork UCC. Dept. Physiology. - [Read Luciferase Assay Protocol for HEK COS cells]

Methods for DNA isolation - Protocol Book - http://www.genome.ou.edu/protocol_book/protocol_partIII.html#III

Category: DNA Protocols: DNA Extraction Protocols

Large scale double-stranded DNA isolation, Midiprep double-stranded DNA isolation. Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman. - [Read Methods for DNA isolation - Protocol Book]

Mini-Prep Protocol - http://www.udel.edu/Biology/fschmieg/midiprep411.htm

Category: Microbiology: Mini-Prep Plasmid Purification

Mini-prep Protocol. Florence Schmieg. University of Delaware. - [Read Mini-Prep Protocol]

Molecular Cloning Lab â€“ Restriction Enzyme Digestion PDF - http://ender.bu.edu/~tgardner/be209/lab_manual/Lab%204.pdf

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Molecular Cloning Lab â€“ Restriction Enzyme Digestion. Molecular Cloning introduction, lab for students - restriction digest exercise with questions. BE209 Spring 2006. Prof. Timothy Gardner. Boston University, Department of Biomedical Engineering. - [Read Molecular Cloning Lab â€“ Restriction Enzyme Digestion PDF]

Paraformaldehyde Fixation of Cells - http://www.biotech.iastate.edu/facilities/CELLHYB/Fixation.htm

Category: Cell Biology and Cell Culture: Flow Cytometry Protocols

Paraformaldehyde Fixation of Cells protocol. This fixation method is good for cells labeled by fluorochrome-conjugated antibodies to membrane antigens. It will stabilize the light scatter and labeling for up to a week in most instances, allowing you to be more flexible in scheduling cytometer time. Furthermore, it inactivates most biohazardous agents, so it is important from a safety standpoint as well. Iowa University. - [Read Paraformaldehyde Fixation of Cells]

PCR Amplification of DNA - http://www.research.umbc.edu/~jwolf/m2.htm#pcr

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

PCR Amplification of DNA. Very Basic Protocol. Julie B. Wolf, University of Maryland. - [Read PCR Amplification of DNA]

PCR Amplification of DNA - http://www.research.umbc.edu/~jwolf/m2.htm#pcr

Category: PCR Protocols: Standard PCR Protocol

PCR Amplification of DNA. Very Basic Protocol. Julie B. Wolf, University of Maryland. - [Read PCR Amplification of DNA]

Polymerase Chain Reaction (PCR) Optimization - http://www.uaf.edu/museum/resumes/cook/mollab/pcr.html

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: PCR Optimization Protocols

Optimizing PCR Reaction Conditions. Optimizing PCR Tips and Guide. University of Alaska Museum - [Read Polymerase Chain Reaction (PCR) Optimization]

Preparation of cytospin from single cell suspension - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/CytospinPreparation.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Preparation of cytospin from single cell suspension. Columbia University. Cattoretti Lab. - [Read Preparation of cytospin from single cell suspension]

Preparing a cell block from cell lines / cell suspensions - http://icg.cpmc.columbia.edu/cattoretti/Protocol/Immunohistochemistry/Cell_block.html

Category: Molecular Imaging: Immunofluorescence Microscopy

Preparing a cell block from cell lines / cell suspensions. Cattoretti, Colombia University. - [Read Preparing a cell block from cell lines / cell suspensions]

Processing Adherent Cultured Cells for Tissue Microarray Controls ... - http://www.tissuearray.org/CellLinesProtocolforTMA112.pdf

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Cell Fixation. 1. Gently wash cells 2x in sterile 1XPBS, ... [Take to Research Histology for embedding] Instructions: Embed pellets in vertical ... Jena Giltnane Yale University Dept. of Pathology. Processing Adherent Cultured Cells for Tissue Microarray Controls. - [Read Processing Adherent Cultured Cells for Tissue Microarray Controls ...]

Protein extraction from whole tissues for isoelectric focusing - http://proteomics.missouri.edu/protocols/proteinExtraction.html

Category: Protein Protocols: Protein Extraction From Tissue

Protein extraction from whole tissues for isoelectric focusing. University of Missouri - Columbia Proteomics Center. SDS extraction followed by acetone precipitation. Also has Phenol extraction followed by methanolic ammonium acetate precipitation. - [Read Protein extraction from whole tissues for isoelectric focusing]

Protein Isolation and Analysis From Plants - http://csm.jmu.edu/biology/courses/bio480_580/mblab/protein_isolation.htm

Category: Protein Protocols: Protein Extraction From Tissue

Protein Isolation and Analysis From Plants. T. Rife. Department of Biology, James Madison University. Harrisonburg, VA - [Read Protein Isolation and Analysis From Plants]

Protein Precipitation Protocols - http://www.ls.huji.ac.il/~purification/Protocols/ProteinPrecipitation.html

Category: Protein Protocols: Protein Precipitation Procols

TCA-DOC Normal, TCA Acetone Precipitation, Ethanol Precipitation, TCA-DOC / Acetone, Acidified Acetone/Methanol, Dr. Mario Lebendiker. University of Jerusalem. - [Read Protein Precipitation Protocols]

Protocol for Manual Peptide Synthesis - http://www.biotech.unl.edu/proteomics/protocol.html

Category: Peptide Protocols

Protocol for Manual Peptide Synthesis. Resin Swelling And Coupling Of Activated Amino Acid Esters. Deblocking and cleaving peptide from solid support. Synthesis of Peptides. The University of Nebraska-Lincoln Protein Core Facility - [Read Protocol for Manual Peptide Synthesis]

PROTOCOL FOR SDS-PAGE - http://www.fiu.edu/~animals/protocols/sds_page.html

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

PROTOCOL FOR SDS-PAGE. Immuno-Physiology Laboratory, Florida International University. Includes: Primary considerations, preparing samples, loading samples, running gels, and quantitative staining. - [Read PROTOCOL FOR SDS-PAGE]

Purification of poly(A)+ RNA from total RNA - http://kuchem.kyoto-u.ac.jp/seika/shiraishi/protocols/oligotex.html

Category: RNA Protocols: mRNA Extraction Protocols

Purification of poly(A)+ RNA from total RNA. Hideaki Shiraishi, Kyoto University. The protocol employs Oligotex-dT30 for poly(A)+ RNA purification. - [Read Purification of poly(A)+ RNA from total RNA]

Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA by Centrifugation - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3916

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: DNA Plasmid Preps Protocols

Contamination of plasmid DNA by fragments of DNA and RNA is reduced to an acceptable level by centrifugation through 1 M sodium chloride. This method was devised by Brian Seed when he was a graduate student at Harvard University. - [Read Removal of Small Fragments of Nucleic Acid from Preparations of Plasmid DNA by Centrifugation]

reparation of Copy Standards for Southern Blot Copy Number Determination - http://www.med.umich.edu/tamc/spike.html

Category: Transgenic Mouse Protocols: Mouse Genotyping Protocols

PCR screens must be designed to detect transgene DNA at the single copy level.Copy standards are prepared by mixing non-transgenic tail DNA with a known amount of transgene DNA to produce transgene copy standards. University of Michigan Transgenic Animal - [Read reparation of Copy Standards for Southern Blot Copy Number Determination]

Articles (1-4 of 4)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

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