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Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis - http://www.healthsystem.virginia.edu/internet/biomolec/2dgelsampleprep.cfm

Category: Proteomic Protocols

Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis. Univ. Virginia. - [Read Preparation of whole cell homogenates of cultured mammalian cells for 2-D Electrophoresis]

Radiation Safety Self-Audit Checklist PDF - http://web.princeton.edu/sites/ehs/radiation/RadiationLab.pdf

Category: Radioactivity: Radioaction Safety

Radiation Safety Self-Audit Checklist PDF. EHS Princeton Univ. - [Read Radiation Safety Self-Audit Checklist PDF]

Restriction Endonuclease Digestion of DNA - http://www.cf.ac.uk/biosi/staff/kille/Methods/Gene/Restriction_Digests.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

Restriction Endonuclease Digestion of DNA. Double and Single Digestions. Cardiff Univ. PK group - [Read Restriction Endonuclease Digestion of DNA]

Restriction enzyme digestion of DNA: basic method - http://www.methodbook.net/dna/restrdig.html

Category: Molecular Biology Protocols: Restriction Enzyme Digestion

How much DNA to digest? Master mix for restriction digest. Double digests. Star activity. Matt Lewis, Department of Pathology, Univ. Liverpool. - [Read Restriction enzyme digestion of DNA: basic method]

Sandwich ELISA Procedure - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Biochemistry/sELISA.html

Category: ELISA Protocols: Sandwich ELISA Assay Protocols

Sandwich ELISA Procedure. Kitto Lab, Univ. Texas at Austin - [Read Sandwich ELISA Procedure]

Silver Staining of Gels for Mass Spectrometry - http://www.healthsystem.virginia.edu/internet/biomolec/Silverstains.cfm

Category: Mass Spectrometry Protocols

Silver Staining of Gels for Mass Spectrometry. Desiderio procedure: R.R. Becklin. Univ. Virginia. - [Read Silver Staining of Gels for Mass Spectrometry]

Standard Agarose Gel Electrophoresis - http://haveylab.hort.wisc.edu/protocol/gel.html

Category: Molecular Biology Protocols: Agarose Gel Preparation

Gel Recipes, Sub-Mini Agarose Gel Electrophoresis. Michael Havey's Vegetable Genetics Laboratory, Univ. Wisconsin. - [Read Standard Agarose Gel Electrophoresis]

Sterile Technique for DNA and Protein Expression - http://structbio.vanderbilt.edu/chazin/wisdom/labpro/sterile.html

Category: General Research Protocols and Methods: Sterile Technique

Sterile technique is one of the most important steps in insuring consistent results when employing recombinant DNA and protein expression techniques. Dr. Chazin Lab, Center for Structural Biology, Vanderbilt Univ. - [Read Sterile Technique for DNA and Protein Expression]

Tandem Affinity Purification (TAP) Protocol - http://depts.washington.edu/~yeastrc/ms_tap1.htm

Category: Mass Spectrometry Protocols

Tandem Affinity Purification (TAP) Protocol. Modified from Rigaut et al. 1999. Univ.Washington Yeast Resource Center. - [Read Tandem Affinity Purification (TAP) Protocol]

Tissue Culture Methods - http://www.research.umbc.edu/~jwolf/method5.htm#tc%20methods

Category: Cell Biology and Cell Culture: Cell Culture Techniques

Tissue Culture Methods. Julie B. Wolf, Univ. Maryland. Protocols for Counting Cells, Preservation and storage of cell lines, maintenance of cell lines, media and growth, and safety guidelines for working with cells. - [Read Tissue Culture Methods]

Topo Cloning Procedure - http://preuss.bsd.uchicago.edu/protocols/topo.html

Category: DNA Protocols: TOPO Cloning

Topo Cloning procedure for cloning of small amounts DNA fragments by PCR. Cloning the PCR fragment into the TOPO vector, transforming E. Coli cells, and using blue/white selection to determine transformants. Culture inoculation, Qiagen Plasmid Prep protocol for plasmid isolation of the plasmid containing the cloned copies. Preuss Lab, Univ. Chicago. - [Read Topo Cloning Procedure]

TOPO Cloning Simple Protocol PDF - http://www.colorado.edu/MCDB/odorizzilab/TOPOcloning.pdf

Category: DNA Protocols: TOPO Cloning

TOPO Cloning Simple Protocol. PDF Odorizzi Lab. Univ. Colorado. - [Read TOPO Cloning Simple Protocol PDF]

Tryptic digestion directly in polyacrylamide gel slices - http://www.healthsystem.virginia.edu/internet/biomolec/ingeldigest.cfm

Category: Mass Spectrometry Protocols

Tryptic digestion directly in polyacrylamide gel slices. A. Shevchenko et al. Univ. Virginia. - [Read Tryptic digestion directly in polyacrylamide gel slices]

UV Spectrophotometric Analysis of DNA and RNA - http://www.people.hofstra.edu/faculty/Beverly_Clendening/Adv_Molecular_Biology/Protocols/UV_Spec_Analysis_RNA&DNA.htm

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Determining the concentration of a RNA or DNA sample by using UV spectrophotometry. Hofstra Univ. Beverly Clendening. - [Read UV Spectrophotometric Analysis of DNA and RNA]

Wet-lab protocols - http://eagle.mmid.med.ualberta.ca/private/labnotes/wetlab/index.html

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

LB plates, LB medium (for 1 liter), Agarose gels. Bart.Hazes Univ Alberta. - [Read Wet-lab protocols]

Yeast Two-Hybrid Screen with Library and Bait - http://faculty.virginia.edu/mirmira/resources_files/Protocols/YEAST%20TWO.htm

Category: Molecular Model Organisms: Yeast Two-Hybrid Screen Protocol

yeast transformed with bait vector. yeast culture. selection plates. Mirmira, Univ. Virginia. - [Read Yeast Two-Hybrid Screen with Library and Bait]

Articles (1-4 of 4)

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

In Vitro Translated Xenopus Mos Kinase Assay Protocol

In Vitro Translated Xenopus Mos Kinase Assay Protocol. In response to progesterone, immature Xenopus oocytes mature to eggs that can be fertilized. The Mos protein kinase is essential for oocyte maturation, most likely due to its ability to activate the MAP kinase cascade. This MAP kinase cascade eventually leads to the activation of Cdc2/cyclin B and entry into M phase. In this protocol, tagged Mos kinase is translated in vitro, immunopurified, and used in a kinase assay.

Antibodies Phage Expression Protocol Using 96-well Microtiter Plates

A Phage display expression protocol for antibody expression in 96-well microtiter plates.

Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

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