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PCR Amplification of DNA - http://www.research.umbc.edu/~jwolf/m2.htm#pcr

Category: DNA Protocols: PCR Polymerase Chain Reaction Protocols: Standard PCR Protocol

PCR Amplification of DNA. Very Basic Protocol. Julie B. Wolf, University of Maryland. - [Read PCR Amplification of DNA]

PCR Amplification of DNA - http://www.research.umbc.edu/~jwolf/m2.htm#pcr

Category: PCR Protocols: Standard PCR Protocol

PCR Amplification of DNA. Very Basic Protocol. Julie B. Wolf, University of Maryland. - [Read PCR Amplification of DNA]

Preparation of Genomic DNA from Bacteria Protocol - http://userpages.umbc.edu/~jwolf/m1.htm#genomic%20DNA

Category: Nucleic Acid Purification Protocols: DNA Isolation Protocols: Genomic DNA Isolation Protocols

Protocol for preparation of genomic DNA from bacteria. - [Read Preparation of Genomic DNA from Bacteria Protocol]

Procedure for Transfection of Mammalian Cells Using Lipofectamine - http://www.research.umbc.edu/~jwolf/m10.htm

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Lipofectamine transient tranfection of mammalian cells in IMDM cell media. Julie B. Wolf, UMBC. - [Read Procedure for Transfection of Mammalian Cells Using Lipofectamine]

Sterile Technique - General Tips - http://www.research.umbc.edu/~jwolf/method2f.htm

Category: General Research Protocols and Methods: Sterile Technique

Sterile Technique - General Tips. Julie B. Wolf, UMBC - [Read Sterile Technique - General Tips]

Tissue Culture Methods - http://www.research.umbc.edu/~jwolf/method5.htm#tc%20methods

Category: Cell Biology and Cell Culture: Cell Culture Techniques

Tissue Culture Methods. Julie B. Wolf, Univ. Maryland. Protocols for Counting Cells, Preservation and storage of cell lines, maintenance of cell lines, media and growth, and safety guidelines for working with cells. - [Read Tissue Culture Methods]

Transformation of E. coli by Electroporation Protocol - http://userpages.umbc.edu/~jwolf/m7.htm

Category: E Coli Protocols: E coli Transformation Protocols

Protocol for the transformation of E. coli by electroporation. Includes: Preparation of E. coli cells for electroporation; Preparing DNA for Electroporation. - [Read Transformation of E. coli by Electroporation Protocol]

Transformation of E. coli by Electroporation Protocol - http://userpages.umbc.edu/~jwolf/m7.htm

Category: Transfection Protocols: Electroporation Sonoporation Protocols

Protocol for the transformation of e. coli by electroporation. - [Read Transformation of E. coli by Electroporation Protocol]