ultrafiltration Protocols

Category: Nucleic Acid Purification Protocols

Ultrafiltration is an alternative to ethanol precipitation for the concentration and desalting of nucleic acid solutions.  It requires no phase change and is particularly useful for dealing with very low concentrations of nucleic acids.  This protocol describes the use of the Microcon cartridge, a centrifugal ultrafiltration device, to concentrate and desalt nucleic acid solutions. - [Read Concentrating and Desalting Nucleic Acids with Microconcentrators Protocol]

Category: Yeast Protocols: Yeast Genetics Protocols: YACs Protocols

Protocol describes a method for purification of YAC DNA using ultrafiltration. This is the fastest method available, but care needs to be taken not to damage the DNA. The purified DNA can then be used for microinjection. - [Read Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units Protocol]

Category: Nucleic Acid Purification Protocols

In this protocol, ultrafiltration through Centricon or Microcon concentrators is used to remove unused primers, primer-dimers, and NTPs from preparations of amplified DNA. - [Read Removal of Oligonucleotides and Excess dNTPs from Amplified DNA by Ultrafiltration Protocol]