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5' RACE Protocol For Generation of Seq. Tags - http://baygenomics.ucsf.edu/protocols/comp1/RACE_generation.pdf

Category: RNA Protocols: 5' RACE - Rapid Amplification of cDNA Ends Protocols

Can also use 96-well plates. First Strand cDNA Synthesis, First Size selection, second 2nd strand cDNA synthesis. Bay Area Functional Genomics Consortium, UCSF. - [Read 5' RACE Protocol For Generation of Seq. Tags]

AMAD: Another Microarray Database. - http://derisilab.ucsf.edu/microarray/software.html

Category: DNA Microarray Protocols: DNA Microarray Software

DNA Microarray Software. Data Analysis, Another Microarray Database AMAD, Cluster, Gal File Maker, ScanAlyze. DeRisi Lab. - [Read AMAD: Another Microarray Database.]

ANTIBODY PURIFICATION by affinity chromatography - http://mullinslab.ucsf.edu/protocols/html/ANTIBODY_PURIFICATION_by_affini.htm

Category: Chromatography Protocols: Immunoaffinity Chromatography Protocols

ANTIBODY PURIFICATION by affinity chromatography. By Beth, Mullins Lab UCSF. To affinity purify antibodies, generate lots of E. coli lysate that contains your antigen. If the protein can stand freeze thawing, then go ahead and purify the protein from e. coli lysate and keep it frozen until you need to couple it to a CH-sepharose column. - [Read ANTIBODY PURIFICATION by affinity chromatography]

ArrayMaker Version 2.60 - http://derisilab.ucsf.edu/arraymaker.shtml

Category: DNA Microarray Protocols: DNA Microarray Software

DNA Microarray Software, ArrayMaker Version 2.60. DeRisi Lab. - [Read ArrayMaker Version 2.60]

Calcium Phosphate Transfection of Neurons in Primary Culture - http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/Ca_P_TX_R.shtml

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Detailed Neuronal cell transfection protocol and methods. Steven Finkbeiner. UCSF. - [Read Calcium Phosphate Transfection of Neurons in Primary Culture]

CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA Protocol - http://waldman.ucsf.edu/Protocols/indirectcgh.html

Category: Cytogenetics Protocols

Protocol for CGH of biotin labeled DNA vs digoxigenin labeled DNA. Includes: Reprecipitate DNA's; Denature slides; Hybridization; Washing and staining. - [Read CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA Protocol]

CGH of Direct Labeled Test DNA vs Normal DNA Protocol - http://waldman.ucsf.edu/Protocols/directcgh.html

Category: Cytogenetics Protocols

This CGH Protocol is used for DNA of good quality when available in sufficient amounts. We usually do replicate hybridizations using samples labeled "inversely" (reversing the label for test and normal DNA's). If appreciable artifact occurs, then alternative labels are tried. - [Read CGH of Direct Labeled Test DNA vs Normal DNA Protocol]

Clone Genes From a Phage Library Protocol - http://www.ucsf.edu/micro/faculty/Johnson/protocols/protocol1.html

Category: DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols

Protocol for cloning genes from a phage library. Includes: Titer and plate out phage; Lift plaques onto filters and prepare them for screening; Make a probe; Hybridize the probe to the filters; Wash the filters and expose to film; Purify putative plaques; Excise plasmid from the desired phage. - [Read Clone Genes From a Phage Library Protocol]

Electroporation and isolation of ES cell lines Mouse - http://baygenomics.ucsf.edu/protocols/comp1/electroporation.html

Category: Cell Biology and Cell Culture: ES Stem Cell Biology and Culture

Electroporation and isolation of ES cell lines Mouse. BayGenomics. - [Read Electroporation and isolation of ES cell lines Mouse]

Frozen Sectioning and Tissue Sectioning for Electron Microscopy - http://www.ucsf.edu/pathol/research/morphprotocol.htm

Category: Histology Protocols: Tissue Sample Sectioning

Frozen Sectioning and Tissue Sectioning for Electron Microscopy. Includes Tissue Processing for Transmission Electron Microscopy and Tissue Preservation for Light Microscopy. University of California UCSF Dept. Pathology Research. - [Read Frozen Sectioning and Tissue Sectioning for Electron Microscopy]

Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF - http://derisilab.ucsf.edu/pdfs/BeadBeatIP_Protocol.pdf

Category: DNA Protocols: Chromatin Immunoprecipitation Protocols

Chromatin-IP (or ChIP) has been a useful method for the localization of proteins to specific regions of DNA. ChIP applied to microarrays (ChIP on Chip) Brown Lab - [Read Immunoprecipitation of Chromatin from Fixed Yeast Protocol PDF]

List of DNA Microarray Protocols - http://derisilab.ucsf.edu/microarray/protocols.html

Category: DNA Microarray Protocols

List of DNA Microarray Protocols. microarrays.org - [Read List of DNA Microarray Protocols]

Preparation of Dialysis Tubing - http://mullinslab.ucsf.edu/protocols/html/Preparation_of_Dialysis_tubing.htm

Category: Protein Protocols: Protein Dialysis Protocols

Preparation of Dialysis Tubing. Mullins Lab. UCSF - [Read Preparation of Dialysis Tubing]

Purification of GST-fusion proteins E. coli. (pGEX vector) Protocol - http://mullinslab.ucsf.edu/protocols/html/GST_fusion_expression.htm

Category: E Coli Protocols: E coli Protein Protocols

Protocol for the purification of GST-fusion proteins E. coli. (pGEX vector). - [Read Purification of GST-fusion proteins E. coli. (pGEX vector) Protocol]

Quantitation of DNA, RNA and ss Oligos - http://mullinslab.ucsf.edu/protocols/html/QUANTITATION_OF_DNA_AND_RNA_AND.htm

Category: General Research Protocols and Methods: Spectrophotometry Protocols

Quantitation of DNA, RNA and ss Oligos. Mullin Lab, UCSF. - [Read Quantitation of DNA, RNA and ss Oligos]