ucsd Protocols

Category: Cell Biology and Cell Culture: Cell Transfection Protocol

Transfection of DNA into cells using PIBS, DNA, CaCl2 and Calcium phosphate. Daniel O'Connor, UCSD Chromaffin Cell and Hypertension Research - [Read CaPO4 Transfection for Chromaffin Cells]

Category: C. elegans Protocols: C. elegans Fixing Protocols

Parameters for fixation and staining. Includes: Antigen, Fixation, Fixation time, Antibody and Dilution. - [Read Fixation and Staining Parameters]

Category: Immunology: Immunocytochemistry Protocols

Method for plating cells, fixing cells, antibody incubation and mounting of slides. PHEM buffer, Antifade recipes. Howell Lab. - [Read Immunocytochemistry]

Category: C. elegans Protocols

Protocol for immunofluorescence of C. elegans embryos. - [Read Immunofluorescence of C. elegans Embryos Protocol]

Category: Peptide Protocols: Antibodies from Peptides Protocols

Protocol for Antibody Creation from a Synthetic Peptide. Antibody from Peptides. Daniel O'Connor. - [Read Making antibodies to synthetic peptides]

Category: Antibody Protocols: Antibody Production Protocols

Protocol for making antibodies to synthetic peptides. - [Read Making Antibodies to Synthetic Peptides Protocol]

Category: C. elegans Protocols: C. elegans Staining Protocols

Protocol for PAR-1 staining procedure. Includes fixation and staining. - [Read PAR-1 Staining Procedure Protocol]

Category: DNA Protocols: SAP Protocols

Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.). Shirley Reynolds Hendrick Lab. - [Read Phosphatasing with Shrimp Alkaline Phosphatase (S.A.P.)]

Category: Signalling Signal Transduction Protocols: Protein Kinase Protocols

Protein Kinase C Assay, Newton lab. Using the peptide substrate Ac-FKKSFKL-NH2. Detailed Assay Protocol. - [Read Protein Kinase C Assay]

Category: Luciferase and Dual Luciferase Assays

Protocol: Luciferase assay protocol. Simple Recipies for Luciferase assay buffers I and II. - [Read Protocol: Luciferase assay protocol]

Category: Protein Protocols: Protein Electrophoresis: SDS-PAGE Electrophoresis Protocols

SDS-PAGE with Low Toxicity Staining Protocol. Chad Rappleye, Aroian Lab Protocols - [Read SDS-PAGE with Low Toxicity Staining]