tumefaciens Protocols

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Root Transformation of Arabidopsis Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4671

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Arabidopsis can be stably transformed using Agrobacterium tumefaciens-mediated transfer of T-DNA. We describe the generation of transgenic plants via root transformation in tissue culture, which can be useful for transforming sterile mutants. - [Read Root Transformation of Arabidopsis Protocol]

Simplified Arabidopsis Transformation Protocol - http://plantpath.wisc.edu/~afb/protocol.html

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

Agrobacterium tumefaciens strain transformation of arabidopsis thaliana. Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. - [Read Simplified Arabidopsis Transformation Protocol]

Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.ip29

Category: Plant Biology Protocols: Arabidopsis Transformation Protocols

A. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial DNA (the transfer, or "T," DNA) into the plant cell genome. The Ti plasmid also carries many of the transfer functions for mobilizing the T-DNA. This article provides a brief discussion of the principles of T-DNA transformation, including consideration of T-DNA vectors and their hosts. - [Read Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol]