User Panel

My Panel

Bookmark Science Articles

tsy Protocols

Search results for: tsy

Protocols » Search Results

Featured Search Results Links

Molecular Station Bioinformatics - http://www.molecularstation.com/bioinformatics/link/

Category: Bioinformatics

Molecular Station Bioinformatics - [Read Molecular Station Bioinformatics]

Real Time PCR Bioinformatics and Databases - http://www.molecularstation.com/bioinformatics/link/Real_Time_PCR/

Category: Real Time PCR

Real Time PCR Bioinformatics and Databases. Molecular Station. - [Read Real Time PCR Bioinformatics and Databases]

Protein Protocols MolecularStation - http://www.molecularstation.com/protein/protein-protocols/

Category: Protein Protocols

Protein Protocols MolecularStation. Western Blotting, Immunoprecipitation, stripping and reprobing - [Read Protein Protocols MolecularStation]

Protein and Antibody Microarray Chips - An Introduction - http://www.molecularstation.com/protein-microarrays/

Category: Protein Microarray Protocols

Protein Microarray Chips - An Introduction. Introduction, Types of Protein Chips, Attachment, Protein and Antibody Chip Production, Applications of Protein Chips, Detection methods, and Future Directions. Molecularstation. - [Read Protein and Antibody Microarray Chips - An Introduction]

Western Blot Home - http://www.molecularstation.com/protein/western-blot/

Category: Protein Protocols: Western Blot Protocol

Western Blot Home. Information on western blotting, western blot procedure and methods, western blot books, stripping protocols. - [Read Western Blot Home]

Methylation and Epigenetics Bioinformatic Tools - http://www.molecularstation.com/bioinformatics/link/Epigenetics_and_Methylation/

Category: Epigenetics and Methylation Protocols

Methylation database, CpG Island predictors, Methylation Primer design tools and databases. Epigenetic tools and bioinformatics. - [Read Methylation and Epigenetics Bioinformatic Tools]

Troubleshooting Western Blot - http://www.molecularstation.com/protein/troubleshooting-western-blots/

Category: Protein Protocols: Western Blot Protocol: Troubleshooting Western Blots

Covers Many Western Blot Problems and Includes Many Solutions. Fuzzy Bands, Low or Weak Signal, High Background, Spots on Film, Too Many Bands. A MolecularStation Guide. - [Read Troubleshooting Western Blot]

Peptide Station - http://www.peptidestation.com

Category: Peptide Protocols

Peptide Bioinformatics and Protocols. Peptide News, Articles, and Custom Peptide Synthesis Vendors and Services. - [Read Peptide Station]

Insomnia Sleep - http://www.insomniasleep.com

Category: Affiliates

Insomnia Sleep Information for people having trouble sleeping. Latest research on insomnia and sleep deprivation. - [Read Insomnia Sleep]

Diabetes Post - http://www.diabetespost.com

Category: Affiliates

Diabetes Post provides information and news on diabetes mellitus and insipidus types I and II. A forum for diabetes discussion. - [Read Diabetes Post]

Western Blot Info - http://www.westernblotinfo.com

Category: Protein Protocols: Western Blot Protocol

Western Blot Information, forum, and protocols. - [Read Western Blot Info]

ELISA assay - http://www.elisaassay.com

Category: ELISA Protocols

ELISA Assay. Learn about the ELISA assay. Includes ELISA assay protocols and methods. - [Read ELISA assay]

Search Results Protocol Links Sort by: Hits | Alphabetical

Affinity Chromatography Handbook - http://books.google.com/books?id=kUl4k55F-fIC&pg=PA169&lpg=PA169&dq=dna+affinity+chromatography+protocol&source=web&ots=A7TBT29Ejf&sig=XkdH0cM4tNPKdN7y9XN2BfNMeno#PPA79,M1

Category: Chromatography Protocols

Affinity Chromatography Handbook. Google Books. - [Read Affinity Chromatography Handbook]

Affinity Chromotography Magnetic Bead Purification of RNA-binding proteins from Rat Brain. - http://147.52.72.117/IJMM/2003/volume11/number4/509.pdf

Category: RNA Protocols: RNA-binding Protein Purification

Affinity Chromotography Magnetic Bead Purification of RNA-binding proteins from Rat Brain. Scaturro et al. 2003. - [Read Affinity Chromotography Magnetic Bead Purification of RNA-binding proteins from Rat Brain.]

Affinity Purification of Antibodies - http://cmgm.stanford.edu/~vesicle/protocols/affinity.html

Category: Protein Protocols: Antibody Purification Protocols

Affinity Purification of Antibodies. Vesicle Trafficking. - [Read Affinity Purification of Antibodies]

Affinity purification of antibodies from crude serum - http://wheat.pw.usda.gov/~lazo/methods/au/sect32.html

Category: Protein Protocols: Antibody Purification Protocols

Affinity purification of antibodies from crude serum. David Bowtell Lab. - [Read Affinity purification of antibodies from crude serum]

Affinity purification of antibodies Protocol - http://www.xenbase.org/xmmr/Marker_pages/methods_pages/affinity_col.html

Category: Protein Protocols: Antibody Purification Protocols

Affinity purification of antibodies. Protocol by Peter Vize, XMMR - [Read Affinity purification of antibodies Protocol]

Affinity Purification of Interacting Proteins from Cell Lysates Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/3/pdb.prot4593

Category: Chromatography Protocols: Affinity Chromatography Protocols

Recombinant protein or a chemically synthesized bioactive fragment is immobilized on resin and used as a probe to capture interacting proteins directly from a cell extract. Affinity-purified proteins are fractionated by gel electrophoresis and visualized by Coomassie staining. Proteins that interact specifically are identified by comparing this gel profile to one obtained from cell lysates passed over a control resin lacking the immobilized probe protein. - [Read Affinity Purification of Interacting Proteins from Cell Lysates Protocol]

Affymetrix Real Time PCR information and optimization - http://keck.med.yale.edu/affymetrix/rtpcr/

Category: Real Time PCR

Affymetrix Real Time PCR information and optimization. Yale U. Keck Foundation. - [Read Affymetrix Real Time PCR information and optimization]

AFLP - Genetic Fingerprinting - http://www.keygene.com/technologies/technologies_aflp.htm

Category: PCR Protocols: AFLP PCR

AFLPÂ® technology was developed initially by Keygene in the early 1990â€™s. AFLP has become a widespread genetic fingerprinting technology. AFLP video and diagrams. Keygene - [Read AFLP - Genetic Fingerprinting]

AFLP For Positional Cloning - http://www-ciwdpb.stanford.edu/publications/methods/aflp.html

Category: Plant Biology Protocols: Plant Genetics Protocols

AFLP was designed as a highly sensitive method for DNA fingerprinting to be used in a variety of fields. We are using this technology to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been identified genetically by mutant phenotype. Protocol includes: Generate polymorphic recombinant F2 (or F3) population; Isolate genomic DNA; Restriction of DNA; Ligation of adapters; Pre-amplification of template DNA; AFLP-PCR; etc. - [Read AFLP For Positional Cloning]

AFLP genotyping and fingerprinting Review PDF - http://taxonomy.zoology.gla.ac.uk/~rdmp1c/teaching/L4/Evolution/Session5/Mueller.pdf

Category: PCR Protocols: AFLP PCR

Ulrich G. Mueller and L. LaReesa Wolfenbarger. Amplified fragment length polymorphisms (AFLPs) are polymerase chain reaction (PCR)-based markers for the rapid screening of genetic diversity. AFLP. TREE October 1999 - [Read AFLP genotyping and fingerprinting Review PDF]

AFLP Method - http://haveylab.hort.wisc.edu/protocol/AFLP.html

Category: PCR Protocols: AFLP PCR

AFLP Procecure, includes restriction Digestion of Genomic DNA. Michael Havey - Vegetable Genetics Laboratory - [Read AFLP Method]

AFLP PCR Protocol PDF - http://www4.ncsu.edu/~wagebrey/AFLP-Salm.pdf

Category: PCR Protocols: AFLP PCR

Restriction digestion, Adapter ligation, Preamplification, Final Amplification steps for AFLP PCR. Detailed protocol. Wondwossen Abebe Gebreyes - [Read AFLP PCR Protocol PDF]

AFLP protocol - http://www.msu.edu/user/hazensam/aflp/AFLPprotocolMSU.html

Category: PCR Protocols: AFLP PCR

Restriction Digestion of Genomic DNA , Adapter preparation, Ligation of Adapters. Pre amplification Reactions. Gel electrophoresis, Silver Staining. AFLP Protocol and procedure. - [Read AFLP protocol]

AFLP protocol Wolf Lab - http://bioweb.usu.edu/wolf/aflp_protocol.htm

Category: PCR Protocols: AFLP PCR

Restriction and Ligation reactions, Setting up the reactions, AFLP PCR reactions protocols. Analyzing the data using Genescan. Paul G. Wolf, Utah State Univ. - [Read AFLP protocol Wolf Lab]

AFLP's - http://opbs.okstate.edu/~melcher/mg/mgw1/MG11128.html

Category: PCR Protocols: AFLP PCR

AFLP is a variation of RAPD. Facts of AFLP PCR. Ulrich Melcher. - [Read AFLP's]

AFLP: a new technique for DNA fingerprinting. - http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=7501463

Category: PCR Protocols: AFLP PCR

P Vos, R Hogers, M Bleeker, M Reijans, T van de Lee, M Hornes, A Frijters, J Pot, J Peleman, and M Kuiper. 1995. Nucleic Acid Research. The AFLP technique is based on the selective PCR amplification of restriction fragments from a total digest of genomic - [Read AFLP: a new technique for DNA fingerprinting.]

AFLP: not only for fingerprinting, but for positional cloning - http://www-ciwdpb.stanford.edu/publications/methods/aflp.html

Category: PCR Protocols: AFLP PCR

Mannie Liscum and Paul Oeller. Department of Plant Biology. Carnegie Institution of Washington, Stanford. AFLP technology is used here to generate DNA based markers for cloning genes involved in phototropic responses in higher plants that have only been i - [Read AFLP: not only for fingerprinting, but for positional cloning]

AFLPs on the ABI 3100 - http://www.maizemap.org/aflp.htm

Category: PCR Protocols: AFLP PCR

AFLPs on the ABI 3100. Maize Mapping Project - [Read AFLPs on the ABI 3100]

Agar Plates for Selection of Clones in Bacteria - http://www.le.ac.uk/biology/phh4/bactplte.htm

Category: Microbiology: Bacterial E.Coli Culture Media & Plates

LB Agar: Luria Broth, X-Gal preparation, IPTG and ampicillin. Petri dish preparation. Pat Heslop-Harrison and Trude Schwarzacher, Univ. Leicester - [Read Agar Plates for Selection of Clones in Bacteria]

Agarose Gel DNA Recovery Protocol - http://rothlab.ucdavis.edu/protocols/DNA-from-Agarose-Review.html

Category: DNA Protocols: DNA Extraction Protocols: Agarose Gel DNA Extraction Protocols

Agarose Gel DNA Recovery Protocol - Recovering DNA from agarose gels. Paul N. Hengen, Ph.D - [Read Agarose Gel DNA Recovery Protocol]

Agarose Gel Electrophoresis PDF - http://www.uni-tuebingen.de/evoeco/files/methods/Electrophoresis.pdf

Category: Molecular Biology Protocols: Agarose Gel Preparation

Agarose Gel Electrophoresis. Pouring of Agarose Gel, Preparation of samples, Gel electrophoresis, Ethidium bromide staining. - [Read Agarose Gel Electrophoresis PDF]

Agarose Gel Electrophoresis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4020

Category: Nucleic Acid Electrophoresis Protocols

Protocol on how to pour, load, and run an agarose gel. - [Read Agarose Gel Electrophoresis Protocol]

Agarose Gel Electroporesis of DNA Protocol - http://www.unc.edu/~fbottone/protocol/electro.html

Category: Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Agarose Gels Protocols

Protocol for agarose gel electrophoresis of DNA. Includes: Making the gel; Loading the gel; Running the gel.
- [Read Agarose Gel Electroporesis of DNA Protocol]

Agarose Gel Preparation Pictures - http://www.biotech.iastate.edu/publications/ppt_presentations/html/Fingerprinting/AgaroseGelPrep/02.html

Category: General Research Protocols and Methods

Agarose Gel Preparation Pictures. Mike Zeller - [Read Agarose Gel Preparation Pictures]

Agarose Gel Preparation Protocol - http://fp.bio.utk.edu/mycology/Techniques/mt-agarose_gels.htm

Category: Molecular Biology Protocols: Agarose Gel Preparation

Solutions for Agarose gel prepartion, Selection of Appropriate Gel Size and Composition. Mycology Lab University of Tennessee - [Read Agarose Gel Preparation Protocol]

Articles (1-10 of 39)

Populational Analysis of Genomic DNA Protocol

A protocol on the populational Analysis of genomic DNA.

Preparing Stock Plating of Bacteria Protocol

A protocol for the preparation of stock plating of bacteria.

Lambda Phage Protocol for Large Preparation DNA Recovery

Lambda Phage Protocol for Large Preparations DNA Recovery.

Lambda Phage Protocol Large DNA Preparation

Single Stranded Plasmid DNA Isolation Protocol

A Single Stranded Plasmid DNA Isolation Protocol describing the production and isolation of single-stranded DNA (ssDNA) using bacteriophagemid-containing bacteria and helper phage. Infection of the host cells with helper phage allows for packaging of ssDNA into bacteriophage. The ssDNA can then be isolated from phage particles.

Genomic DNA Labeling of Microarrays Protocol

DNA microarrays are an ordered arrangement of DNA molecules complementary to genes of interest that are "spotted" by robotic equipment onto a glass slide substrate. The expression of genes in cells can be monitored with microarrays by preparing cDNA from the mRNA of cells of interest and measuring the hybridization to the microarray. This protocol describes the labeling of genomic DNA for use as a probe for hybridization to the cDNA spotted on the array.

Drosophila Cell Transfection Protocol

A simple Drosophila tissue culture cell transfection method.

Tubulin Polymerization Protocol using GTP and GMPCPP

Tubulin is polymerized into microtubules by incubating tubulin at 37°C with GTP. A nucleation seed is added when the purpose is to assay microtubule elongation. Tubulin can also be polymerized for the purposes of recycling the tubulin or labeling the microtubules with fluorescently labeled tubulin. Based on the protocol by Timothy Mitchison of Harvard University.

Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA

This Microarray Protocol Preparation of Fluorescent DNA Probes from Human mRNA protocol describes the production of probes labeled with the fluorescent dyes, Cy3 and Cy5, following the synthesis of cDNA from human mRNA and the hybridization of the probes to DNA microarrays.