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AR Protease Digestion Method - http://www.chemicon.com/techsupp/Protocol/Protease.asp

Category: Immunology: Antigen Retrieval

Protease Antigen Retrieval (Trypsin) Protocol. Chemicon. - [Read AR Protease Digestion Method]

FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING - http://www.vetmed.auburn.edu/index.pl/cell_cycle/dna_content

Category: Histology Protocols: Fixation of Cells Tissues Protocol

Analysis of Cell Cycle Based on DNA Content. FIXATION PROTOCOL FOR FLOW CYTOMETRIC ... Liberate cells from tissue culture flask by trypsin digestion and ... FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING. Auburn University, College of Veterinary Medicine. You. - [Read FIXATION PROTOCOL FOR FLOW CYTOMETRIC ANALYSIS OF DNA BY PI STAINING]

G-Banding Observations - http://info.med.yale.edu/genetics/ward/tavi/slide08.html

Category: Cytogenetics Protocols

G-Banding observations. Includes: Slide storage; Fixative composition and trypsin treatment in G-banding; Banding of chemically aged slides. - [Read G-Banding Observations]

In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis - http://web.archive.org/web/20010906052645/http://www.pharma.ethz.ch/bmm/protocols/ingeldig.html

Category: Proteomic Protocols: 2-D Gel Electrophoresis

In gel trypsin digestion for Mass spectrometry. (According to EMBL Protein and Peptide Group, 1997) - [Read In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis]

Passage of Adherent Cell Lines (subculture). - http://www.hyclone.com/pdf/procedure_passage.pdf

Category: Cell Culture Protocols: Insect Cell Culture Protocols

Remove medium from culture container, rinse with PBS, expose cells to trypsin, incubate, stop trypsin activity by adding serum containing medium, dissociate cells, dilute and return to incubator. - [Read Passage of Adherent Cell Lines (subculture).]

Proteomic Protocols for Sample Submission to Mass Spec - http://www.cfgbiotech.com/proteomics/protocols.htm

Category: Proteomic Protocols

Proteomic Protocols for Sample Submission to Mass Spec. Gel Staining, In gel trypsin digestion, Coomassie Blue staining and TCA precipitation. CFG U. Albany. - [Read Proteomic Protocols for Sample Submission to Mass Spec]

Staining Chromosomes (G-Banding) Protocol - http://humgen.wustl.edu/hdk_lab_manual/cam/cam4.html

Category: Cytogenetics Protocols

Protocol for staining chromosomes (G-Banding). Includes: Trypsin (1:250) USP Grade; Gurr Buffer Solution: (1 liter); Working Salt Solution: (1 liter); Leishman Stain Solution. - [Read Staining Chromosomes (G-Banding) Protocol]

Unmasking Hidden Epitopes with Proteases Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4528

Category: Immunohistochemistry Protocols: Antigen Retrieval Protocols

Fixation can mask epitopes. However, it is often possible to re-expose them using a gentle incubation with proteases, which removes obstructing structures and allows antibody access, as described here. Many proteases can be used for this procedure, including very crude preparations of proteases, such as pronase. However, using a better-characterized protease, such as trypsin, allows a more controlled reaction and better comparison between experiments. - [Read Unmasking Hidden Epitopes with Proteases Protocol]

Worthington Tissue Dissociation Guide - http://www.tissuedissociation.com/techniques.html

Category: Cell Culture Protocols

Includes information on: Cell Isolation Theory ( Tissue Types: Epithelial Tissue, Connective Tissue); Dissociating Enzymes (Collagenase, Trypsin, Elastase, Hyaluronidase, Papain, Chymotrypsin, Deoxyribonuclease I, Neutral Protease (Dispase), Trypsin Inhibitor (Soybean)); Cell Isolation Techniques: Working With Enzymes, Basic Primary Cell Isolation, Equilibration with 95%O2:5%CO2, Trituration, Enzymatic Cell Harvesting, Cell Adhesion and Harvesting, Trypsin for Cell Harvesting, etc... - [Read Worthington Tissue Dissociation Guide]

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Immobilized Antigen Phage Antibody Selection Protocol

A protocol for the selection of Phage Antibodies using Immobilized Antigen. This method describes the selection of antibodies from bacteriophage antibody libraries that recognize a specific antigen. The phage display library of antibody-displaying phage particles is exposed to antigen attached to a solid substrate (Nunc Immuno™ tubes). The phage particles with affinity for antigen bind to the immobilized antigen and are selected from the library of phage expressing antibodies.

Histone H1 Kinase Activity Assay

Histone H1 Kinase Activity Assay Protocol. This protocol describes assaying kinase activity of a putative kinase using Histone H1 as the substrate. Histone H1 is the canonical kinase substrate in this type of assay. Phosphorylation of Histone H1 is assessed by SDS-Polyacrylamide gel electrophoresis followed by autoradiography.

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