Skin fibroblasts are incorporated into 3-D collagen lattices containing the test compounds. An inhibition of lattice contraction indicates a possible toxic effect which is verified by trypan blue exclusion for cell viability. - [Read Human Skin Fibroblast/Collagen Lattice Cytotoxicity Test]
Trypan blue viability test- http://web.archive.org/web/20030430191647/http://www-ufk.med.uni-rostock.de/lablinks/protocols/e_protocols/trypan.htm